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Result show:the solution,extracted from rat rail tendons,consist mainly of type I collagen;No growth of bacteria in 37℃ thermostatic culture box for 72 hours;Absorbing the height of ultraviolet-spectrophotometry is 230 nm by using flicker photometer examination;Amino acid in type I collagen consist of aminoacetic acid(40 per cent) and proline(14.3 per cent);Under microscope:collagen gel netted constraction consist of original collagen fibril that had been fairly well-distributed and had a quite regular striate lines ;The collagen solution prepared collagen gel matrixes in 37℃ thermostasis for culture cells and also prepared collagen solid film by washing collagen gel with a phosphate solution for plasty surgery materials.

结果:提取液主要为Ⅰ型胶原蛋白;72小时37℃恒温培养箱内培养无细菌生长;紫外分光光度计测定,吸收高峰为230nm;氨基酸成分主要为甘氨酸:占40%,脯氨酸:14.3%。显微镜下见:相互交织的胶原原纤维结构组成的网状结构;纤维均匀,有规则横纹。胶原蛋白液在37℃恒温下,可制成凝胶,用于培养细胞;用磷酸液冲洗胶原蛋白凝胶可成固体片状,制成胶原膜,用作医用材料。

From the commencement of week 3,the cartilage showed the characteristic of later OA morphologically with fibrosis of cartilage,sclerosis of sunchondral bone,amalgamation,opening and fibrosis of marrow cavity.

从第3周开始,正常的软骨细胞基本消失,呈纤维样变,软骨下骨硬化;髓腔融合、开放、纤维化,呈现晚期骨性关节炎的改变。

Here we show that senescent cells accumulate in murine livers treated to produce fibrosis, a precursor pathology to cirrhosis.

在这里我们展示在鼠类的肝脏中堆积衰老细胞用于使肝脏纤维化,肝脏纤维化是肝硬化的前驱病理改变。

This compound staining method has multicolor and fine contrast, and can better show melanins, the red blood cells at hemorrhagic focus, and collagenic and elastic fibers.

能够较好的显示皮肤组织中含有黑素细胞颗粒、胶原纤维及出血部位的红细胞和弹力纤维组织成分。

A special staining method for the simultanouse demonstration of nodal cell, myordiac cell, collagenic and elastric fibers was applied on a same sheet of glass. This technique is simple and dired. There is contrast sharply with color.

本文建立了一种研究心脏传导系统和心血管形态学的特殊染色技术(改良的Masson's三色法十Verhoeff's弹力染色法),能在同一玻片上将起搏细胞、工作心肌、胶原纤维及弹力纤维区分开来,且操作简捷,色彩对比鲜明。

We describe a case of NLPHL showing a variant histologic pattern characterized by small and large clusters of lymphocyte predominance cells within nodules defined by follicular dendritic cell meshworks, associated with numerous neutrophils and areas of internodular fibrosis. Focally, the neoplastic cells had lacunar cell-like cytologic features.

在此,我们描述一例组织形态特殊的NLPHL,其形态学表现为以淋巴细胞为主的细胞排列成大小不等的结节,结节均由滤泡树突状细胞网网罗,结节内见中性粒细胞浸润,部分结节间见纤维化,局灶瘤细胞呈陷窝样。

Methods: Immunohistochemical study of CD133 expression: 55 cases osteosarcoma conventional paraffin-embedded tissues specimens come from Pathology Department Qilu Hospital Shandong University, including 4 cases parosteal osteosarcoma, 13 cases fibroblast-type osteosarcoma , 12 cases chondroblastic osteosarcoma and 26 cases Osteoblastoma osteosarcoma.

免疫组化研究CD133的表达:取自本院病理科的骨肉瘤常规石蜡包埋组织蜡块标本55例,其中骨旁骨肉瘤4例,纤维母细胞型骨肉瘤13例,软骨母细胞型骨肉瘤12例,骨母细胞型骨肉瘤26例,切成3μm的组织切片备用。

Many tumor specific antigens such as mouse PlA, human MAGE, BAGE and GAGE families had been isolated during recent decade. PlA is a tumor specific antigen expressed by mouse mastocytoma P815. It is one kind of cancer-testis antigens and expresses in various mouse tumor such as mastocytoma P815, plasmoma J558 and fibrosarcoma Meth A but hardly in normal tissue except testis and placenta.

近十年来已经成功分离出多种鼠或人的肿瘤特异性抗原,如小鼠P1A基因和人的MAGE、BAGE、GAGE家族等。P1A为小鼠肥大细胞瘤P815所表达的肿瘤特异性抗原,它是一种CT抗原,在多种小鼠肿瘤如肥大细胞瘤P815、浆细胞瘤J558和纤维肉瘤Meth A等中表达,而在除睾丸、胎盘之外的正常组织中不表达。

In the SMC of the normal rabbits, myofilaments and macula densa were abundant, few mitochondrla and endoplasmic reticulums were found around the nuclear, and the SMC was in the contractive stage. In the SMC of the model rabbits, few myofilaments and macula densa were found, rough surfaced endoplasmic reticulums and mitochondria were abundant, some phagocytosed much lipid and became foam cells, the SMC was in the synthetic stage.

实验12周,高脂组家兔主动脉斑块面积百分比为47.69±12.84%,内膜斑块厚度为0.68±0.08mm,内膜斑块横断面积与中膜面积比值为1.28±0.35;内膜脂质斑块弥漫且较厚,有大量的泡沫细胞堆积,可达8~10层,管腔直径变小,内膜不光滑;泡沫细胞内富含脂滴,呈椭圆形气球样,有的斑块中可见显著的平滑肌细胞增生及大量的弹力纤维形成。

The present study is designed to seek the evidences of regeneration of auditory epithelia and acoustic nerve in the postnatal chick, By using the methods of tissue cultures, scanning electron microscopy, autoradiography and the cell image analysis technique, we observed the regeneration of auditory hair cell after intense sound exposure, and also neuron repair following a mechanical injury with either in vivo or in vitro.

本研究应用物理性噪声,建立了内耳听器-基底乳头声损伤的模型和初级听神经元纤维机械损伤的模型。在此基础上应用体外组织细胞培养、扫描电镜、放射自显影、光镜以及细胞图象分析等多种形态学方法,综合分析了听觉毛细胞和初级神经元轴突的再生反应。

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