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The process of EMT is induced in epithelial cells to lose its cell-to-cell adhesion and transform to mesenchymal-like cells.

同时,近端肾小管的纤维母细胞亦经类似 EMT 之机制而转变成纤维原母细胞,此也是造成肾病变的因素。

Under optical microscope, the cell of NS arrayed as nest, the nuclear was big, deep-stained, thickened-karyotheca, chromatin granulated, Pathologic karyokinesis can be seen.

DDP组癌巢周围可见少量纤维组织增生、局灶性坏死,并可见炎细胞浸润;LNT组镜下见少量细胞凋亡,凋亡细胞变小,变圆,核固缩,胞浆浓缩,染色加深,凋亡细胞周围未见炎症反应;LNT+DDP组与LNT组镜下大体相同,但可见到较多量的凋亡细胞。

Each group with drug had effect on inhibiting Lewis lung cancer tumor,LNT DDP had the best effect,46.2%,the rate of inhibitory effect of LNT、DDP was 35.6%、39.7%respectively.3.Under optical microscope,the cell of NS arrayed as nest,the nuclear was big,deep-stained,thickened-karyotheca,chromatin granulated,Pathologic karyokinesis can be seen.

DDP组癌巢周围可见少量纤维组织增生、局灶性坏死,并可见炎细胞浸润;LNT组镜下见少量细胞凋亡,凋亡细胞变小,变圆,核固缩,胞浆浓缩,染色加深,凋亡细胞周围未见炎症反应;LNT DDP组与LNT组镜下大体相同,但可见到较多量的凋亡细胞。

Carbon black particles had propensity to redeposit on the fiber surface or inside the fibers and those redeposited ink particles were very difficult to be dislodged. The redeposition of carbon black particles whose size ranged between 0.5μm and 3μm was the main reason for brightness loss.

水性油墨粒子易于沉积在纤维表面或细胞腔内,而且一旦油墨粒子沉积于细胞腔内便很难被去除,直径在0.5~3μm之间的水性油墨粒子再沉积于纤维表面或内部是导致白度降低的主要原因。

E and Sirus red in supersaturated picric acid solution;the amount of HA in serum was measured with the method of radiative immunity;the quantitative expression of fibrosis factor—TGF、PDGF was measured with the method of immunity histochemistry.Results:Myocardial collagen in model group increased obviously compared with normal group from the results of electron microscope、H.E、Sirus red in supersaturated picric acid solution.

腹腔注射柯萨奇B3病毒建立病毒性心肌炎慢性期心肌纤维化小鼠模型,随机分为模型对照组、通心络低剂量组、中剂量组、高剂量组,并设正常对照组,用药四周后处死动物通过透射电镜观察心肌细胞超微结构;HE染色、苦味酸天狼星红染色观察心肌胶原数量;放免法测定血清透明质酸的含量;免疫组化染色观察致纤维化细胞因子—转化生长因子和血小板衍生生长因子的定量表达。

Methods:Establish the model of myocardial fibrosis in chronic stage of viral myocarditis by injecting CVB3 into Balb/c rats,divide these rats into model group、Tong xin luo low dose group、Tong xin luo medium dose group、Tong xin luo high dose group and set up normal group,after four weeks of giving medicine execute the animals and observe the ultrastructure of myocardial cell with tramission electron microscope,the quantity of myocardial collagen was observed by staining with H.E and Sirus red in supersaturated picric acid solution;the amount of HA in serum was measured with the method of radiative immunity;the quantitative expression of fibrosis factor—TGF、PDGF was measured with the method of immunity histochemistry.

腹腔注射柯萨奇B3病毒建立病毒性心肌炎慢性期心肌纤维化小鼠模型,随机分为模型对照组、通心络低剂量组、中剂量组、高剂量组,并设正常对照组,用药四周后处死动物通过透射电镜观察心肌细胞超微结构;HE染色、苦味酸天狼星红染色观察心肌胶原数量;放免法测定血清透明质酸的含量;免疫组化染色观察致纤维化细胞因子—转化生长因子和血小板衍生生长因子的定量表达。

MethodsNeural tubes isolated from the region between midotic placode and third somite of mouse embryo at ED 8.5d were cultured in DMEM/F12 serum-free medium containing basic fibroblast growth factor and epidermal growth factor. The cell cycle of CNCCs was analyzed with flow cytometry, the proliferation capability was determined by MTT method, AP-2α expression was detected by immunocytochemistry as a biological marker of cardiac neural crest cells. The features of migration, multi-directional differentiation were observed as well.

材料与方法成年2月龄C57/BL6小鼠交配,取ED8.5d小鼠胚胎枕中部至第3体节神经管,应用包含碱性纤维生长因子和表皮细胞生长因子的无血清培养基培养,利用MTT法检测细胞增殖能力,利用流式细胞仪检测细胞周期,采用转录激活因子2α(AP-2α,Activator protein-2α)作为其生物学标记物,观察其增殖状况、细胞周期、迁移、分化等生物学特性。

In the leaves of Liriodendroideae, some of the abaxial epidermal cells are papillose and the vascular tissue of the main vein appears to be separated. However, papillose were not found and there are uniseriate, multicellular or unicellular hairs distributed on the epidermis, and the vascular tissue of the main vein appears to be continuous in the leaves of Magnolioideae. Furthermore, in the Magnolioideae, the structure of the leaves of plants in Manglietia are different from that of Magnolia.

结果表明:鹅掌楸亚科和木兰亚科在叶的结构上的主要区别是鹅掌楸亚科两种植物叶的部分下表皮细胞呈乳突状,且整个细胞外壁只形成一个乳突,而在木兰亚科的植物中有单列多细胞或单细胞的表皮毛,未发现乳突;鹅掌楸亚科植物的叶主脉维管组织环分隔呈束状,且其外包被的纤维也排列成束状,而木兰亚科的80种1亚种植物中,叶主脉维管组织连成轮状,其外面也由一圈连续的纤维环所包围。

Objective To report 1 case with cardial malignant fibrohistiocytoma, and discuss its clinical pathological features based on literature review.

目的 报告1例罕见的心脏恶性纤维组织细胞瘤,结合文献复习,讨论心脏恶性纤维组织细胞瘤的临床病理特点。

It is further confirmed by the experiment of muti-inducement that MSMPs can differentiate into osteocyte sand fibrocartilage cytes and it is osteo-chondro progenitor cells with the potential ability to evolve muti-differentiated. This added evidence to the view that condylar cartilage differentiated from osteo-chondro progenitor cells in the mesenchymal cells of mancibular periosteal.

体外分离培养鼠胚下颌体骨膜间质细胞并研究其生物学特性,通过多向诱导实验进一步证实:下颌骨膜间质细胞可能为骨—软骨的前体细胞,可分化为成骨细胞及纤维软骨细胞,具有多向分化潜能,此结果进一步支持髁突软骨是由下颌骨膜间质中骨—软骨前体细胞发育而来。

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