纤维细胞的

RESULTS: The collapse was obvious at weeks 2 and 6 after modeling, with only few granulation tissues and fibrous tissues. No chondrocytes were observed. At weeks 12 and 18, fibrous tissues were found at the defect site, and bone tissue and cartilage proliferation was observed at the bottom and margin. However, it was greatly different from normal cartilage. Moreover, there was still no chondrocytes.

结果：实验兔后腿关节的股骨髁关节面的关节软骨缺损后，在制模后2，6周缺损部位凹陷明显，只有少许肉芽组织和纤维组织生长填充，未见软骨细胞爬行生长，12，18周见缺损部位以纤维组织增生为主，底部及边缘可见骨组织和软骨增生，但是镜下形态仍与正常软骨组织区别较大，表面仍未见软骨细胞爬行生长。

RESULTS: SEM results showed that the fiber morphology of P(50/50) 6% nanofibers membrance was better and more uniform diameter distribution. Mechanic test indicated nanofibers membrance of P(50/50) had longer breaking elongation but smaller breaking stress than nanofibers membrance of P(75/25). Hydrophilicity of P(75/25) fibers was better. MTT results proved that 6% and 8% P(50/50) nanofibers could enhance the adhesion and proliferation of endothelial cells on the surface of materials, and the cells had the best spreading and attachment on the nanofibers.

结果：通过扫描电镜观察发现由质量分数为6%聚(50/50)制备的纤维膜具有更好的纤维形态，且直径分布均匀；拉伸力学测试显示由聚(50/50)制备的纤维膜比聚(75/25)具有更高的断裂伸长率，但断裂应力较低；细胞生物相容性实验表明猪髋动脉内皮细胞在质量分数为6%和8%聚(50/50)的纳米纤维膜上更能有效的黏附与增殖。

In this article, we used automotive immunohistochemical stainer to check the expressions of HBsAg and HCV antigens in 100 cases of HCC and their adjacent tissues, mean while, we checked their histological activity index and hepatic fibrosis degree. We also used radioimmunoassay to detect serum levels of 4 serum fibrosis markers and serum AFP levels in these cases. Immunofluorescence labeling and flow cytometry were used to check the T-cell subpopulations and the activity of NK cells of these 100 cases. Then the correlations among these factors were studied.

本文采用全自动免疫组化仪对100例肝癌组织及癌旁组织中的HBsAg、HCV抗原表达进行了标记，同时对其进行肝组织活动指数、纤维化分期；同时采用放射免疫测定方法检测同期血清透明质酸、Ⅲ型前胶原蛋白、层粘连蛋白和Ⅳ型胶原蛋白等四项血清肝纤维化标志物和血清甲胎蛋白水平；采用免疫荧光标记技术和流式细胞仪对T细胞亚群及NK细胞活性测定，对各项指标的相关性进行系列对比研究。

Since 1962, when Gross and Lapiere discovered the first MMPs-collagenase, man had discovered various of MMPs in succession, where MMP-2 has a strong degradational effect on extracellular matrix, it is secreted bymacrophage, smooth muscle cells and endothelial cells and so on.

自从 1962年 Gross和 Lapiere发现第一种基质金属蛋白酶一胶原酶以来，人类陆续发现了很多种基质金属蛋白酶，其中的MMP—2对细胞外基质具有极强的降解作用，它由巨噬细胞、平滑肌细胞以及内皮细胞等分泌，在动脉粥样硬化斑块中憎多时降解纤维帽及基底膜的胶原等基质成分，是使纤维帽变薄及加速动脉粥样硬化的重要因素之一。

Methods Nuclear β-catenin expression was detected by immunohistochemistry in 77 lesions with desmoid-type fibromatosis and 171 other spindle cell lesions, including superficial fibrmoatosis (n=18), nodular fasciitis (n=36), keloid (n=16), scar (n=10), granulation tissue (n=9), synovial sarcoma (n=38), neufibroma (n=13), solitary fibrous tumor (n=12), gastrointestinal stromal tumor (n=10), low-grade myxofibrosarcoma (n=3), low-grade fibromyxoid sarcoma (n=3), and smooth muscle tumor (n=10). In addition, the immunohistochemical expressions of ER-α, ER-β and Ki-67 were examined in all of the lesions with desmoid-type fibromatosis. The nuclear immunohistochemical staining for nuclear βcatenin and ER-β was graded as high level (≥25% of cells), low level (5%-25%) or none.

采用免疫组织化学染色法检测77例韧带样型纤维瘤病和171例其它良、恶性梭形细胞病变（包括结节性筋膜炎36例、浅部纤维瘤病11例、瘢痕疙瘩16例、增生性瘢痕10例、肉芽组织9例、滑膜肉瘤38例、神经纤维瘤13例、孤立性纤维性肿瘤12例、胃肠间质肿瘤10例、低度恶性黏液纤维肉瘤3例、低度恶性纤维黏液样肉瘤3例及平滑肌肿瘤10例）组织中β-catenin核阳性的表达，同时检测韧带样型纤维瘤病中ER-α、ER-β和Ki-67的表达。

Furthermore,the changes of cell organelles realized different cell functions, which fiber for mechanical support,parenchyma cell for preservation and vessel for transportation.3.FE-SEM and DCR technique revealed the arrangements of CMfs on cell wall of fiber, parenchyma cell and vessel.Firstly,fiber cell formed multilamellate concentric structure with thin and thick lamellae alternate.

纤维、基本薄壁组织细胞、导管分子的终端分化就是PCD；并且PCD过程是一个具有较长周期的过程，周期长短比较为基本薄壁组织细胞＞纤维＞导管分子；三类细胞最后的凋亡决定于细胞内原生质体完全降解的时刻。

The fiber length has only little influence on the basic density within the growth rings, and significant correlation at 0.01 levels was found between the basic density and the fiber length among the different rings. Only slight negative correlation was found between the basic density and the fiber width within the growth rings, but significant positive correlation at 0.01 levels was indicated between the basic density and the fiber width among the growth rings, contrary to that of fiber length. It was demonstrated that significant positive correlations at 0.01 levels between the basic density and fiber double wall thickness, fiber length to width ratio and double wall thickness to diameter ratio, significant negative correlations at 0.01 levels between the basic density and fiber diameter and diameter to width ratio, only slight negative correlation between the basic density and fiber width both in the same growth rings and among the different growth rings. No significant correlation was found between the basic density and the vessel morphological features, nor was the tissue proportion in the same growth rings. But among the different rings, it was found there was significant positive correlation at 0.01 levels between the basic density and the fiber proportion among the different rings, and significant negative correlation at 0.01 levels between the basic density and vessel-elements proportion and ray proportion, only slight negative correlation between the basic density and the parenchym proportion. Significant or no significant negative correlation was found between the basic density and the microfibril angle in the same growth rings, but significant negative correlation was found between the basic density and the microfibril angle among the different growth rings.

生长轮内纤维长度对基本密度的影响不大，而在不同生长轮间纤维长度与基本密度达极显著正相关，纤维宽度与此相反，同一生长轮内纤维宽度与基本密度极显著负相关，不同生长轮间只有微弱负相关；基本密度与纤维双壁厚、长宽比、壁腔比在生长轮内和生长轮间均呈极显著正相关，而与胞腔直径、腔径比均呈极显著负相关，仅与纤维宽度呈微弱的负相关；导管形态对基本密度的影响不显著；同一生长轮内组织比量对基本密度的影响也不显著，但不同生长轮间基本密度与纤维比量呈极显著正相关，与导管比量和木射线比量呈极显著负相关，与轴向薄壁细胞比量仅呈不显著负相关；生长轮内基本密度与微纤丝角呈显著或不显著负相关，但在生长轮间这种负相关达到极显著水平。

Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400

图1 SHEE培养在盖坡片上，活细胞单层生长，胞浆较丰富，细胞核大小一致，有核仁×400 图2 SHEE培养细胞细胞核椭圆形，核仁较大，胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状，有伪足贴壁，表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁，呈星状或多角形，有丰富伪足和胞浆突，核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞，胞核和胞浆PI染色呈红色或淡红色，蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞，染色质凝集呈块状，右上角有一固缩细胞核TdT标记×400

In conclusion, during the development of the mandibule, condylar cartilage derived from MSMPs at the end of the body mandibule. It can evolve into fibrocartilage cytes on appropriate condition and regenerate fibrocartilage provided with proper the three-dimension scaffold materials.

综上所述，下颌发育过程中，髁突软骨来源于下颌体部末端的骨膜间质细胞，此间质细胞在适当的条件下即可向纤维软骨细胞分化，并且此间质细胞在适当的三维支架中可再生纤维软骨。

Computer and image analysis technology were used in this instrument to measure the morphological parameters of the fibers such as fiber length, fiber width, cell wall thickness, fiber coarseness, fibers proportion in the furnish, fibrillating degree, curl index, contents of fines and parenchyma cells.

介绍了一种新型的造纸纤维测量仪，该仪器将计算机及图像处理技术用于造纸纤维形态参数的测量，如纤维长度、宽度、细胞壁厚度、纤维粗度、纤维配比、打浆帚化率、纤维弯曲指数、细小纤维和杂细胞含量等，并兼有摄像和打印功能。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。