纤维细胞的

Because different cell lines may show various levels of susceptibility to adenovirus infection and virus production, non-selectively replicating wtAd5 was used to be a comparison with the tested conditionally replicating adenovirus CNHK500.ONYX-015, the first replication-competent adenovirus entered into clinical trials was also used to be a comparison. We used virus proliferation assay, cell viability assay and western blot to evaluate the proliferation and cytolysis selectivity of CNHK500. Results demonstrated that the CNHK500 virus proliferation multiples in breast cancer cell lines after 48 hours is similar to that of wtAd5, ONYX-015 virus proliferation ability is less than that of CNHK500 in cancer cells.

然后，通过与ONYX-015（E1B 55 KD a蛋白缺失的2型和5型嵌和型腺病毒，美国ONYX生化制药公司研制）、wtAd5进行对比，利用病毒增殖实验和细胞生长抑制实验，验证了CNHK500选择性增殖能力和肿瘤特异性杀伤作用；利用Western Blot检测CNHK500腺病毒E1A和E1B在乳腺癌细胞和正常成纤维细胞中的表达差异，揭示了腺病毒肿瘤靶向性的机制；利用携带绿色荧光蛋白的CNHK500-EGFP感染乳腺癌细胞株和正常成纤维细胞株，直观地观察其感染乳腺癌细胞的能力和在乳腺癌细胞中的增殖过程。

The basic pathological change of silicosis is the lung interstitial fibrosis and the formation of silicotic nodule.

与矽肺的发病相似，特发性肺纤维化也是以肺成纤维细胞大量增殖为主要特征的肺纤维化疾病。

In both inflammatory and healthy gingival epithelium, CD14 mRNA positive cells, which are mainly located at thornlike cell layer, were found.

另一方面，牙髓组织除树突状细胞外CD14表达细胞很少，牙龈组织皮下的成纤维细胞和未分化细胞也不表达CD14。

With the increase of cell density, soma was slenderer and slenderer and like fibroblast.② Growth curves of PDMCs: Growth latency ranged from 2 to 8 days after cell inoculation. During this period, adherence was observed gradually but not obviously amplified. Eight days later, cells entered log growth phase.

结果：①多分化潜能细胞的原代培养情况：胎盘组织消化后获得细胞中仅有少量的贴壁细胞，经两周后逐渐形成扁平单层细胞，呈漩涡状生长或成簇生长，随着细胞密度的增加，胞体变得细长，形态类似成纤维细胞。

METHODS By means of cell culture and flow cy-tometry we studied the effect of fluid nitrogen cryopreser-vation on the expression of MHCⅠantigen of smooth mus-cle cells and fibroblasts of aortic homografts of rats.

采用细胞培养技术和流式细胞仪技术研究液氮低温保存对大鼠主动脉平滑肌细胞和成纤维细胞主要组织相容性抗原Ⅰ类表达的影响。

METHODS By means of cell culture and flow cytometry we studied the effect of fluid nitrogen cryopreservation on the expres sion of MHC Ⅰ antigen of smooth muscle cells and fibroblasts of aortic homografts of ra ts.

采用细胞培养技术和流式细胞仪技术研究液氮低温保存对大鼠主动脉平滑肌细胞和成纤维细胞主要组织相容性抗原Ⅰ类表达的影响。

RESULTS Expression rate of MHCI antigen of cultured smooth muscle cells and fibroblast was（88.7±4.3）%,（84.8±2.0）%respectively;and that of the revived smooth muscle cells and fibroblasts of aortic homografts after cryopreservation was（89.4±3.5）%,（81.9±2.7）%respectively.

结果 正常培养的大鼠主动脉平滑肌细和成纤维细胞MHCⅠ类抗原表达的阳性率分别为（88.7±4.3）%和（84.8±2.0）%；液氮低温保存的大鼠主动脉平滑肌和成纤维细胞在复苏后MHCⅠ类抗原表达的阳性率分别为（89.4±3.5）%和（81.9±2.7）%。

RESULTS Expression rate of MHCI antigen of cult ured smooth muscle cells and fibroblast was (88.7±4.3)%,(84.8±2.0)%respectively; and th at of the revived smooth muscle cells and fibroblasts of aortic homografts after cryoprese rvation was (89.4±3.5)%,(81.9±2.7)%respectively.

结果正 常培养的大鼠主动脉平滑肌细和成纤维细胞MHC Ⅰ类抗原表达的阳性率分别为(88.7±4.3)％和(84.8±2.0)％；液氮低温保存的大鼠主动脉平滑肌和成纤维细胞在复苏后MHC Ⅰ类抗原表达的阳性率分别为(89.4±3.5)％和(81.9±2.7)％。

This study was conducted to examiune the fibrotic effect of Ni-Ti and 317L al loys in esophagus.The extract fluid from Ni-Ti,317L alloys was made according t o the ASTM standards of U.S.A. The Fb of esophageal scar was cultured primarily ,then incubated with alloy abstract fluid. The proliferating activity of Fb was measured by MTT at 4, 24, 48, 72 hours in the course of culturing. The esophagu s embedding test of Ni-Ti,317L alloys was made according to ASTM standards of U .S.A.The tissue around the alloys was taken at weeks 2 and 12,and the pathologi c changes were analysed.

为探讨新型支架材料Ni-Ti、317L合金在食管局部的致纤维化作用，按美国ASTM标准制备NiTi、317L合金的金属浸提液；&组织块培养法&原代培养食管壁疤痕的成纤维细胞，传代后以金属浸提液进行培养，分组后分别培养4、24、48、72 h，MTT法检测不同培养时间后Fb增殖功能的变化；按美国ASTM标准进行NiTi、317L合金试件的食管壁内包埋实验，即将金属试件经表面处理后直接置入食管壁粘膜层与肌层之间，术后2、12周取出包埋组织，分析试件周围组织的病理变化，并进行胶原纤维染色，观察纤维形成状况。

Basic fibroblast growth factor ; lymphokine activated killer cells ; cell division ; interleukin 2; bladder neoplasms ; immunologic cytotoxicity

碱性成纤维细胞生长因子；淋巴因子激活的杀伤细胞；细胞分裂；白细胞介素-2；膀胱癌；免疫细胞毒性

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。