纤维细胞的

Adding dividedly the solution of two drugs with different concentration into the heperplastic scar fibroblasts and keloid fibroblast which were in logarithmic growth phase. After 48 hours in vitro culture, hydroxyproline colorimetric test was conducted, then statistics comparison was carried out in accordance with hydroxyproline content which was calculated based on OD value.

向对数生长期的增生性瘢痕成纤维细胞及瘢痕疙瘩成纤维细胞中加入不同浓度的两种药液，培养48小时后进行羟脯氨酸比色试验，并将 OD 值依公式换算成羟脯氨酸含量，进行统计学比较。

Results:Wound healing rate in group A was higher than that in group B and the wound healing time was 1～3 days earlier in group A.More active proliferation of epidermic cells around wounds showed in group A.The function of fibroblasts in wounds,collagen synthesis and secretion,were more vigorous in group A than those in group B.

结果：A组创面愈合率高于B组，其创面愈合时间较B组提前1～3 d；创周表皮细胞增殖也较B组活跃，创面成纤维细胞较B组呈现出更旺盛的合成与分泌胶原纤维的功能。

Objective To observe the ultrastructure of supra interspinous ligament of ankylosing spondilitis,to evaluate the role of fibroblasts in ossification of ligament,and to discuss if disorderly arrangement of collagen and sedimentation of calcium granule in AS are induced by fibroblasts.

目的 观察强直性脊柱炎棘上棘间韧带的超微结构和探讨成纤维细胞在韧带骨化中的作用。找出AS韧带中胶原紊乱排列和钙颗粒沉积是否由成纤维细胞活动引起。

Many factors may be involve in the course. To investigate the regulation activity of mesenchymal cells to differentiation of epithelial cells from hair follicle and to study its differentiation property, mesenchymal cells gel was made by nubby dermal papilla cells, free dermal papilla cells, skin fibroblasts. Skin keratinocytes and epithelial cells from hair follicle were inoculated on the gel surface and cultured in air-liquid interface. Three-dimensional model of DPC using to induce epithelial cells differentiation is built in vitro.

为了进一步研究毛囊细胞间的相互作用，探讨毛囊间质细胞对毛囊上皮细胞分化的调节作用，研究毛囊上皮细胞的分化特性，我们利用团块状的毛乳头细胞，游离分散的毛乳头细胞或皮肤成纤维细胞制成间质细胞胶原凝胶，表面接种皮肤角质形成细胞或毛囊上皮细胞，进行气-液界面培养，在体外建立了毛乳头细胞诱导毛囊上皮细胞分化的立体模型。

Skin keratinocytes and epithelial cells from hair follicle organized into epidermoid cyst-like spheroids when cultured on nubby dermal papilla cells gel and epidermoid layer-like structure was formed when they were cultured on free dermal papilla cells and skin fibroblasts gel.

团块状的毛乳头细胞诱导毛囊上皮细胞和皮肤角质形成细胞形成球形结构；而游离分散的毛乳头细胞和皮肤成纤维细胞诱导毛囊上皮细胞和皮肤角质形成细胞形成表皮样层化结构。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Results: The cultured cells showed typical shape and features of fibroblast cell ,which were successfully dissociated and purified.

结果：原代培养的成纤维细胞特征典型，并通过传代培养得到了纯化的成纤维细胞。

Specific inhibition of Hh signalling using small molecule inhibitors, a neutralizing anti-Hh antibody or genetic deletion of smoothened in the mouse stroma results in growth inhibition in xenograft tumour models.

由癌细胞分泌的Hedgehog配体，在肿瘤表皮细胞中未能激活信号作用，而是在&基质&上发挥作用（&基质&由细胞外基质、成纤维细胞、内皮细胞和微血管组成，恶性细胞着床于其中）。

Since an excessive cell growth is considered as the main pathological event, cell proliferation model occupy most of the animal models, and these cells include retinal pigment epithelial cell, fibroblast cell, cartilage cell and vascular endothelial cell.

由于PVR的主要病理变化是细胞的过度增生，因而动物模型多以细胞增生模型为主，细胞种类有视网膜色素上皮细胞、成纤维细胞、软骨细胞、血管内皮细胞等。

Materials and Methods: the HPDLFs were isolated and cultured in vitro from healthy premolars of orthodontic patients, which were 10~14 years old.

材料和方法：取材自临床上10～14岁青少年需要拔除的健康的正畸牙，在体外分离、培养并纯化人牙周膜成纤维细胞，建立人牙周膜成纤维细胞有限细胞系。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。