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Result: Rhesus BMSC can be got in mass by density gradient centrifugation.; Rhesus BMSC can adhere to PLGA fiber quickly after plant and its growth , division ,proliferration is good; Some cells arrange like rosary on PLGA fiber surface, some cells still form the catenate or flaky cell bridge joined among PLGA fiber.

结果:采用密度梯度离心的方法可得到大量增殖能力强的猕猴骨髓基质干细胞;接种后的骨髓基质干细胞很快粘附于PLGA纤维上,生长、分裂、增殖良好,在PLGA纤维表面形成串珠样排列,部分细胞还形成连接在PLGA纤维间的链状或片状细胞桥。

Methods:(1) ADM was produced from swine skins treated with trypsin followed by Triton X-100.(2) type I collagen of mouse tail was extracted by 0.5mol/L acetoacetic acid.(3) Two kinds of ADM were got by being soaked with 0.02% glutaraldehyde for 5~10 min or being soaked with mouse type I collagen for 24h, then preserved at 4℃.

(1)应用胰蛋白酶消化-去污剂法制备ADM;(2)用0.5mol/L乙酸溶液提取制备I型鼠尾胶原;(3)用0.02%戊二醛溶液浸泡ADM5-10min,制成交联型ADM;另一部分ADM用质量分数0.25%的I型鼠尾胶原浸泡24h,制成胶原包埋型ADM,冰冻保存;(4)用酶消化法培养原代成纤维细胞,取第三代增殖期细胞,将其调整至浓度为2×105/ml的成纤维细胞悬液。

Functions: supplement collagenic fibre and effectively restore the damaged collagenic fibre. Provide the skin with moisture and nutrition. Remove the thin wrinkle and dry wrinkle around the eyes, delay consenescence of the eye skin. Stem cell activating factors can quickly activate the dermal cells in all layers, promote the renewal of aging cells and speed up the restoring and composing of collagenic fibre and elastic fiber to restore the aging skin. HA high-effectively supplements moisture that can be maintained for a long time. Grapeseed Oil, VitE and Green Tea Extracts Liquid feature in anti-oxidation, removal of free radicals, improvement of the skin metabolism and circulation, elimination of extra moisture and toxin, recovery of skin energy and vigor. Its long time use will make your skin tight and tender, presenting a new look full of vigorous charms.

产品功效:补充胶原蛋白,有效修复受损胶原纤维,为肌肤提供水分和营养,消减眼部细纹和干纹,延缓眼部肌肤的衰老;干细胞激活素能够快速激活皮肤各层细胞,促进衰老细胞的更新,加速胶原纤维和弹性纤维的修复与合成,修复老化受损肌肤; HA 强效补水,高效保湿;葡萄籽油、 VitE 、绿茶萃取液等抗氧化、清除自由基,改善肌肤的代谢和循环,排除多余水分和毒素,回复肌肤能量与活力,长期使用,将令眼部肌肤紧致细嫩,神采奕奕。

AIM: To investigate the effects of dibairen decoctum on the proliferation of keratinocytes and fibroblasts as well as the potential effects of DBRD on fibroblast contraction.

目的:观察地白忍煎剂对角朊细胞和成纤维细胞增殖的影响以及对成纤维细胞收缩功能的潜在作用。

Establishing experimental animal model of immunized fibrosis could contribute to research on extracellular matri and cytokine. they turns out that the ingredient of Desmodium pulchellum can relieve extra growth and deposit of ECM and adjust the level of relative cytokine, such as TGF-β1 and IFN-γ, benefit to effect of hepatic anti-fibrosis.

结果证明排钱草生物碱类成分能减轻肝纤维化的基本病理改变即减少肝细胞外基质的过度增生和沉积,调节与肝纤维化密切相关的TGF-β1、IFN-γ等细胞因子水平,向着有利于减轻肝纤维化的方向发展。

After selective extraction, the cyst wall of two kind of resting cysts displayed a filament network. In the resting cysts of Tachysoma pellionella and Euplotes encysticus, the filament was located in mesocyst and endocyst respectively. On the other hand, a special filament scaffold region was observed below the cyst wall in two kind of cysts. Compared with the true IF which was located in central part of cyst, the filament of this region was net as well as uniform, and no any cytoplasm residue can be seen in this region. In the resting cyst of Euplotes encysticus, a filamental boundry which partitioned the special filament scaffold region and the true IF region was observed.

两类休眠包囊的包囊壁经生化分级抽提后均显示纤维结构,急纤虫休眠包囊中纤维定位于中间层,包囊游仆虫休眠包囊中纤维定位于内层壁;其次,在两类休眠包囊的包囊壁下,相当于细胞质表质层的位置,均有一个特殊的纤维网络区,与细胞质深层的网络区相比,此部分的纤维纯净、均匀,未见附着细胞器及细胞质残留物,在包囊游仆虫休眠包囊中此纤维网络区与真正的细胞质纤维网络之间由纤维构成的界膜隔开。

The protein levels of periostin in KFb (91.815±0.961) and HFb (70.166±2.250) were both higher than that in SFb (41.011±1.576, P.01). Conclusion: The expressions of periostin were increased in the fibroblasts of hyperplasic scars, which could be inhibited by hycirocortisone.

Periostin蛋白主要分布在成纤维细胞核周围的胞浆中,它在瘢痕疙瘩和增生性瘢痕的成纤维细胞中的表达(分别为91.815±0.961和70.166±2.250)高于在正常皮肤成纤维细胞中的表达(41.011±1.576, P.01),两者分别增加55%和42%。

Coculturing of the transfected fibroblasts with neonatal rat ventricular myocyte cultures resulted in a significant reduction (68%) in the spontaneous beating frequency of the cultures compared with baseline values and cocultures seeded with naive fibroblasts.

结果发现,与基线、与幼稚成纤维细胞共培养相比,转染的成纤维细胞和新生大鼠心室肌细胞共培养导致培养物自发搏动频率的下降(68%)。

Immunohistochemistry were positive for CD34 in some region where the fibroblast actively proliferated, whereas fibrocytes in mature fibrous tissue were negatively stained.

CD34免疫组织化学染色显示一些增生活跃的纤维母细胞明显表达CD34,成熟的纤维组织内的纤维细胞CD34则为阴性。

In contrast, hepatocyte growth factor has shown therapeutic effects on injured renal tubules in animal models.

此项研究中,采用人肾脏成纤维细胞培养系统,探讨肝细胞生长因子对高糖诱导的肾间质成纤维细胞损伤产生的作用。

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