纤维细胞的

aim to study the efficaciousness of wuling cap-sules for hepatic fibrosis in rats.methods rat models with hepatic fibrosis were induced by ccl4 compound factors and then treated with wuling capsules.pathology of liver sections,hyaluronic acid and procollagencontents in rat liver and serum were examined respectively.results pathological examinations showed that the liver cells of mod-el rats were injured seriously.collagen fibers proliferated and dissected hepatic lobules in model rats.wuling capsules could protect liver cells and reduce degrees of hepatic fibro-sis.the best effects were achieved at3.0g?

目的 研究五灵胶囊对大鼠肝纤维化的作用。方法采用以四氯化碳为主的复合因素致大鼠肝纤维化，观察五灵胶囊对肝组织病理学、肝组织和血清中羟脯氨酸、ⅰ，ⅲ型前胶原含量的影响。结果病理学检查显示，模型组大鼠肝细胞损伤严重，胶原纤维大量增生，形成假小叶。五灵胶囊组大鼠肝细胞结构破坏不明显，肝纤维化程度轻，在3.0g？kg-1 时治疗效果最好。

The microstructure of the scaffold was observed under photomicroscope and scanning electron microscopy,and the in vitro degradation time,permeability,tensile-strength,porosity,shrinka...

实验结果显示：支架中的成纤维细胞、脂肪细胞及组织纤维间质完全去除，胶原纤维得到了松散，并维持其原有的天然三维网络多孔结构；该材料透水汽性处于3000g.m-2.d-1左右，适合创面恢复；体外降解时间处于25～50h之间，并可根据需要调整工艺条件控制降解时间；拉伸强度介于10.20～11.50MPa之间，具有良好的拉伸强度；收缩温度介于70～85℃之间。上述结果表明该材料已解决了猪脱细胞真皮基质渗透性差、降解速度过慢的缺点，并且其透气性和拉伸强度高、降解性优良且可控，符合组织工程支架材料的要求。

From the outside to the inside , the body skin is divided into the stratum corneum , stratum lucidum , stratum granulosum , stratum spinosm and stratum basale , after through stratum corneum , stratum lucidum , stratum granulosum , stratum spinosm EGF can reach the stratum basale: accelerate the basal cells prpliferation and differentiation , reverse matured cells' differentiation to make stem cells that will form a " stem cells island " with a certain amount , which accelerate the generation of new cellls , make senescent cells keratinize and shed rapidly , make broken and denatured collagen fibers and elastic fibers repaired , so the skin's elasticity increase , and the wrinkles calm down or disappera gradually .

体表皮由外及里共分为角质层、透明层、颗粒层、棘细胞层、基底层，EGF可以通过透明层、颗粒层、棘细胞层深入肌肤基底层，加速基底层细胞增殖分化，将已经分化成熟的细胞逆分化成干细胞，当达到一定量时，便会形成&干细胞岛&，加速新细胞的生成，让衰老细胞快速角化、脱落，已断裂、变性的胶原纤维和弹性纤维得到修复，皮肤弹性增加，皱纹逐渐平复、消失。

Fibroblasts have the ability to secret many kinds of extracellular matrix and cytokines relevant to hepatogenic differentiation. So, we investigate whether coculture of hADSCs with fibroblasts without cell-cell contact play a role in hepatic differentiation of hADSCs by RT-PCR analysis of hepatic specific gene expression and real-time PCR analysis of Alb expression.

成纤维细胞能够分泌很多利于干细胞向肝细胞分化的细胞外基质和细胞因子，我们采用非接触共培养的方式对成纤维细胞与人脂肪干细胞共培养能否引发其向肝细胞的分化进行了研究。

However, whether the human fibroblasts behave in a similar way has not been reported to our knowledge. In the present study fibroblasts collected from human foreskin were cultured in vitro so as to investigate into the fibro blastic osteogenetic role as well as its mechanism.

本实验包括四个部分：1、人皮肤成纤维细胞体外培养成骨的实验研究。2、猪骨形态形成蛋白（pBMP的提取及生物活性测定。3、pBMP对成纤维细胞成骨影响的实验研究。4、成纤维细胞体外培养过程中骨钙素变化。

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物；培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感，据此可在传代过程中将二者分离纯化，获得纯细胞系；混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞；再加0.15%胰蛋白酶-0.02鞹A 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞，经过2~3 代，即可得到纯化的乳腺上皮细胞系。

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物；培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感，据此可在传代过程中将二者分离纯化，获得纯细胞系；混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞；再加0.15%胰蛋白酶-0.02%EDTA 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞，经过2~3 代，即可得到纯化的乳腺上皮细胞系。

Results The endothelial cell and fibroblast of valves and vessel walls in experimental group were completely decellularized, no cell fragments were retained within the matrix scaffold; collagen fiber and elastin fiber had been preserved with intact structure and wavily arrayed; deoxyribonucleic acid content of valves and vessel walls in experimental group were decreased by 97.58%, 97.25% compared with that of control group.

结果 实验组瓣膜及血管壁中内皮细胞、成纤维细胞去除完全，无细胞核碎片；瓣膜及血管壁胶原纤维和弹力纤维呈波浪状排列、整齐，结构完整；瓣膜、血管壁的基因组脱氧核糖核酸含量分别较对照组下降97.58％和97.25％。

Make a copyof rat pulmonary fibrosis by infusing bleomycin through trachea , isolate and the pulmonary fibroblast, then interfered by atorvastatin ,divided into 3 groups of vacant ,TGF-β1 induced and atorvastatin interceped of different dosage , observe the structure of the cell by fiberscope ,the multiplication of the cell by MTT counting method, the expression of theα-SMA by immunity tissue chemistry staining method .

气管内注入博莱霉素（bleomycin，BLM）复制大鼠肺纤维化模型，并分离培养肺成纤维细胞，用阿托伐他汀对其进行干预，分为空白组、TGF-β1诱导组和不同剂量阿托伐他汀阻断组，用倒置纤维镜观察细胞的形态结构，MTT计数法观察各组细胞的增殖情况，免疫组化染色法定性观察α-平滑肌肌动蛋白(α-smooth muscleactin ，α-SMA)的表达情况。

Studies on mouse nuclear transfer with adult male mouse ear fibroblast cells as donors In this study, C57BL/6 adult male mouse ear fibroblast cells and Kunming mouse MⅡoocytes were used as donors and recipients, respectively, to investigate the effects of different passages of donor cells and electrofusion times on the in vitro development of NT reconstructed embryos, when the oocytes were treated with 3% sucrose.

以成年雄鼠耳成纤维细胞为供体克隆小鼠的研究该研究以C57BL/6 成年雄鼠耳成纤维细胞为供体，昆明白小鼠MⅡ期卵母细胞为受体，研究了3%蔗糖处理卵母细胞去核后，供体细胞不同传代次数和电融合次数对核移植重构胚体外发育的影响。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。