纤维细胞的

4DRG was co-culture with sciatic nerve segment in 10鸖 DMEM;the axons were longer and surround the sciatic nerve segment which was regard as anew evidence for chemotropism.

结果：(1)在无血清条件下单独培养的DRG，背根神经节的轴突数目众多，外形纤细弯曲，不成束，并且施万细胞和成纤维细胞稀少，所以可以排除两者对轴突生长的影响，为观察来源于变性坐骨神经段的可溶性因子对轴突生长的作用提供了有利条件；(2)在无血清条件下DRG和变性坐骨神经段联合培养，①先单独培养DRG，4天后待神经元轴突长出，再与坐骨神经段联合培养，观察到神经元的轴突数目减少，外形挺直，部分轴突之间相互粘附成束；②变性坐骨神经段和DRG同时联合培养，神经元的轴突数目明显减少，外形粗壮，轴突之间相互粘附成束；(3)有血清条件下单独培养DRG，轴突数目较多，外形挺直，长短不一，部分神经元的轴突之间相互粘附，施万细胞和成纤维细胞数目众多，观察到的轴突生长情况受到施万细胞和成纤维细胞的直接或者间接的影响。

Methods: The effects of Opuntia on proliferation of fibroblast were measured with MTT colorimetric assay, and PCNA productions were examined with immunocytechemistry.

以小鼠成纤维细胞株(NIH3T3)为研究对象，采用四氮唑盐比色法测定仙人掌对成纤维细胞增生的影响，采用免疫细胞化学染色法检测成纤维细胞的增殖细胞核抗原的表达。

In order to investigate the inhibitory effect of salvia miltiorrhiza and tetramethyl pyrazine on scartricial fibroblast, the hypertrophic scar tissue of chest was chosen for culture of fibroblasts, and the influence of SM and TP on fibroblasts was observed, The effect of the drugs on the growth of fibroblasts, on DNA synthesis of fibroblasts and on mitosis index of fibroblasts were all determined quantitatively.

摘 要 增生性瘢痕是临床治疗难题之一，目前尚无有效的治疗方法。为了探讨丹参和川芎嗪对瘢痕成纤维细胞的抑制作用，取12例患者胸部增生性瘢痕组织，通过体外成纤维细胞培养，探讨不同药物浓度、不同作用时间对成纤维细胞生长的影响。

When electric fusion method was used for nuclear transfer, the fusion rate (46. 0%), cleavage rate (53. 9%) and blastocyte development rate (10.9%) of adult ear fibroblasts were significantly lower than that of fetal fibroblasts (64. 5%, 70.1%, 21. 6% respectively), fetal skin cells (71. 5%, 70.8%, 22. 1% respectively) and ovary granulosa cells (88. 2%, 79. 1%, 25. 5% respectively). There was no significant difference among other donor cells in the cleavage and blastocyst development rate of resconstituted embryos.

当用电融合法进行核移植时，成体耳部成纤维细胞的融合率（46.0％），卵裂率（53.9％）和囊胚发育率（10.9％）均显著低于胎儿成纤维细胞（64.5％，70.1％和21.6％），胎儿皮肤细胞（71.5％，70.8％和22.1％），以及卵巢颗粒细胞（88.2％，79.1％和25.5％）；另外三种细胞间的卵裂率，囊胚发育率无显著差异，但卵巢颗粒细胞的融合率显著高于胎儿成纤维细胞和胎儿皮肤细胞（88.2％vs 64.4％，71.5％，P＜0.05）。

Results: The study showed that the earliest stromal nodules were fibrous, and with the dual differentiation of myofibroblasts toward both smooth muscle cells and fibrous cells, they developed into fibromyomatous nodules and muscular nodules. The results of reclassifying the 55 nodules immunohistochemically were that 23(42%) was fibrous, 28(51%) fibromuscular nodules and 4(7%) muscular.

结果：结合HE切片和免疫组化结果发现前列腺间质结节的早期为幼稚的纤维性结节，随着结节内肌纤维母细胞向平滑肌细胞和纤维细胞的双向分化，间质结节可继续演化为纤维肌性结节和肌性结节。55个结节按免疫组化分类，纤维性结节占42%，纤维肌性结节占51%，肌性结节占7%。

Based on traditional wood fiber processing way at home and abroad, this paper draws up mathematical description theory of wood fiber, finishes the mathematical description theory of single wood fiber, sum up the reason of low density of wood fiber at microcosmos state, completed the laboratory study on Micron Flake Fiber High Strength Board, set up the forming methodology of this kind of wood-based panel.

本文在总结国内外传统法木纤维加工的基础上提出了木纤维数学描述理论，进行了单个木纤维细胞的数学描述，总结了现在木纤维强度低的微观原因，完成了微米长薄片木纤维高强度人造板的实验室研究，创立了该板种的形成方法学，所提出的MFB形成理论体系和加工方式，是利用木材微纳米技术在人造板加工业中开创出的一片新研究领域，提出了微米纤维细胞劈裂的条件。

A lot of organelles such as mitochondria, rough endoplasmic reticula and Golgi bodies were observed in fiber cell. Then, double karyotheca disappeared, and the organelles disintegrated. Multivesicular bodies appeared in fiber cytoplasm. With the further development of fiber, fiber wall underwent continual thickening with aging, and polylaminate structure gradually appeared. While the agglutinated nucleus, transfer vesicles, plasma membrane and plasmodesmata still presented.

研究发现，次生壁形成早期，细胞核具有双层核膜，染色质凝聚，可见大量的线粒体、粗面内质网和高尔基体等细胞器存在于纤维细胞中；随后，双层核膜消失，细胞器将逐渐解体，多泡体开始出现在纤维细胞的细胞质；随着年龄的增加，纤维细胞壁逐渐增厚，并出现多层结构现象，而运输小泡、细胞膜、胞间连丝和凝聚的染色质将持续存在。

Objective: To explore the mechanisms resulting in the recurrence of urethral scar which make urethral strictures difficult to be cured, a series experiments were conducted to find potential effective factors involved in urethral scar formation and degradation, including the studies of extracellular matrix component of urethral stricture scar, the characteristics of urethral scar fibroblast, and the effects of urine on urethral fibroblast in vitro, as well as the studies to compare the difference of collagenase activity, type Ⅰ collagen and tissue inhibitor of metalloproteinase-1 (TIMP-1) in the tissues and cultured fibroblasts from normal urethra and strictured urethra respectively, and the studies to investigate the effect of antisense TIMP-1 oligodeoxyonucleotide on cell proliferation and collagenase activity of urethral scar fibroblast.

中文题名尿道瘢痕基础研究副题名胶原酶活性，TIMP-1的表达及其反义基因治疗外文题名 Experimental study on urethral scar-activity of collagenase，expression of TIMP-1，and antisense TIMP-1 gene transfection of urethral scar fibroblast 论文作者黄翔导师杨宇如教授学科专业外科学研究领域\研究方向学位级别博士学位授予单位四川大学学位授予日期2002 论文页码总数104页关键词尿道手术瘢痕胶原酶成纤维细胞尿道瘢痕馆藏号BSLW /2003 /R699 /12 目的：研究尿道瘢痕的细胞外基质的组成，尿道瘢痕成纤维细胞的生物学特性以及尿液对其生长的影响；比较胶原酶活性，金属蛋白酶组织抑制因子-1（TIMP-1）以及Ⅰ型胶原含量在尿道瘢痕和正常尿道组织及体外培养的成纤维细胞中的差异；研究反义TIMP-1寡核苷酸对尿道瘢痕成纤维细胞增殖以及胶原酶活性的影响。

Methods: Electrets Teflon PTFE,±300 V,±1 000 V were used to treat 3T3 cells for 24, 48 and 72 h. Then the influences of electrets on cell cycle and surface charge of 3T3 cells were studied by flow cytometry and electrophoresis, respectively.

选用±300 V和±1 000 V驻极体分别作用成纤维细胞24、48和72 h，用流式细胞术和细胞电泳技术测定成纤维细胞的生长周期和细胞表面电荷。

Results: Compared with control group, apoptosis cells increased from 0.5%to 10%(some even to 15%) after 24,48 and 72 h action of -300,-500 and -1 000 V electrets. After action of -500 V PTFE electrets for 48-72 h, fibroblast cells showed characteristic morphological features of apoptosis. These features included chromatin aggregation, nuclear and cytoplasmic condensation and partition of cytoplasm and nucleus into membrane bound-vesicles.

结果：-300、-500和-1 000 V驻极体作用成纤维细胞24、48和72 h以后，与对照组相比，成纤维细胞的凋亡量从0.5％增至10％（部分可达15％）；驻极体作用成纤维细胞48～72 h，出现细胞凋亡特有的形态学特征，即：细胞异染色质边集，细胞裂解，可见凋亡小体。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。