纤维细胞

The operative area was examined with light microscope, electronic microscope, and the proliferating cell nuclear antigenwas detected. Results In CsA group, intraocular pressure was significantly lower than that in NS group(P＜0.05), and more functional filtering blebs were observed. Less fibroplasia and proliferative cells, less bioblast and less active caryotin in fibrocyte were found in CsA group than that in NS group. No serious mcomplicaton was found in both group.

结果 环孢霉素A组眼压控制多较理想，与对照组间的差异有统计学意义(P＜0.05)，大多数形成功能性滤过泡，光镜下见极少量纤维组织增生，电镜下见纤维细胞线粒体少，染色质功能不活跃，增殖细胞阳性率低，与对照组差异有统计学意义(P＜0.05)，且没有浅前房、视网膜损害等严重并发症。

At 1st, 7th,14th,and 30th day after surgery, filtering blebs, intraocular pressure, cornea and ocular fundus were observed.the operative area was examined with light microscope, electronic microscope, and the proliferating cell nuclear antigenwas detected. results in csa group, intraocular pressure was significantly lower than that in ns group(p＜0.05), and more functional filtering blebs were observed. less fibroplasia and proliferative cells, less bioblast and less active caryotin in fibrocyte were found in csa group than that in ns group. no serious mcomplicaton was found in both group.

结果 环孢霉素a组眼压控制多较理想，与对照组间的差异有统计学意义(p＜0.05)，大多数形成功能性滤过泡，光镜下见极少量纤维组织增生，电镜下见纤维细胞线粒体少，染色质功能不活跃，增殖细胞阳性率低，与对照组差异有统计学意义(p＜0.05)，且没有浅前房、视网膜损害等严重并发症。

The histopathologic observations of corneas after penetrating keratoplasty in dogs: Thestructure of wholly transparent graft was the same as that of host cornea apart from the upperdonor-host junction where there was minimal irregularity of the corneal lamellae. However, thecorneal lamellae at the lower donor-host junction were neat. The graft with its central part beingtransparent only showed poor coaptation with recipient, in which the graft shifted inward and had marked wrinkles and folds in Descemet"s membrane. On the back of Descemet"s membranethere was hyperplastic stromal fibers that thickened the graft and partially adhered to iris.

5犬穿透性角膜移植术后角膜病理组织学观察完全透明的植片与植床结构一致，仅两者对合处上部纤维紊乱，而下方纤维排列整齐；中央透明的植片与植床对合不良，植片向植床方向嵌入，后弹力层皱缩、折叠，植片下方因有来自植床的基质纤维而增厚，部分对合处与虹膜粘连；浑浊的植片有比较完整的上皮层，植片约2／3深度的基质丧失原来纤维整齐排列结构，代以大量成纤维细胞增生。

Many factors may be involve in the course. To investigate the regulation activity of mesenchymal cells to differentiation of epithelial cells from hair follicle and to study its differentiation property, mesenchymal cells gel was made by nubby dermal papilla cells, free dermal papilla cells, skin fibroblasts. Skin keratinocytes and epithelial cells from hair follicle were inoculated on the gel surface and cultured in air-liquid interface. Three-dimensional model of DPC using to induce epithelial cells differentiation is built in vitro.

为了进一步研究毛囊细胞间的相互作用，探讨毛囊间质细胞对毛囊上皮细胞分化的调节作用，研究毛囊上皮细胞的分化特性，我们利用团块状的毛乳头细胞，游离分散的毛乳头细胞或皮肤成纤维细胞制成间质细胞胶原凝胶，表面接种皮肤角质形成细胞或毛囊上皮细胞，进行气-液界面培养，在体外建立了毛乳头细胞诱导毛囊上皮细胞分化的立体模型。

Study the bioactivity of the n-HA/PA66 composite and the effects it would be to body's metabolism of calcium and phosphorus ion in vivo.(3) Study the osteo-conductivity and the ability to repair bone defect of the porous n-HA/PA66 composite and the feasibility use it as the scaffold of bone tissue engineering. Objects and Methods as follows: 1.To evaluate the biocompatability of nano-hydroxyapatite crystals and polyamide composite (n-HA/PA66) with the L929 cells.To proceed the morphological observation and take pictures of L929 cells after 1d,2d,4d,and 7d of co-cultured with extract of n-HA/PA66 ,and direct contact with n-HA/PA66.To determine light absorbtion value of every hole under 500 nm with enzyme linked immunity instrument after 1 d,2 d,4 d,and 7 d of contact of n-HA/PA66 extract with L929 cells,and direct contact with n-HA/PA66.In the meanwhile calculate the relative multiplication rate of cells,and evaluate them by six degree tests for cytotoxicity. To investigate the acute and chronic toxic reaction on the whole body induced by the new nano-hydroapatite crystals and polyamide composite(n-HA/PA66)after implanting in vivo and its effects on partial constitution of animal organs after implanting in vivo,and evaluate the potential and degree of subcuticular stimulation reaction.

本实验主要由以下三部分组成：一、n-HA/PA66 复合材料在动物体内、体外的生物相容性及生物安全性评价二、n-HA/PA66 复合材料植入动物体内的生物活性及近期对机体钙、磷代谢影响的实验研究三、网孔 n-HA/PA66 复合材料作为支架修复兔桡骨节段缺损的动物实验研究主要研究目标及方法如下：参照 GB/T16886.5-1997-ISO 10993-5：1992《医疗器械生物学评价细胞毒性试验体外法》之评价标准和要求，采用规定的 L929 细胞(小鼠结缔组织成纤维细胞)，分别经直接接触和材料浸提液与细胞共培养等方式对 n-HA/PA66 复合材料进行细胞毒性测试，采用细胞形态观察法观察两种细胞各组在 24h、48h、72h、5 天后各时相点的细胞形态学变化，并在显微镜下照相，从而对细胞与材料的生物相容性进行定性评价；同时采用细胞生长抑制法，以酶标仪定量测定评价各组 1，2，4，7 天 L929 细胞的相对增殖率，以定量测定并判别材料对细胞的毒性程度。

HE staining revealed that collagen fiber, fibroblasts and granulation tissue and synechia belt around tendons and stomas were apparently less in contrast to control group.

术后4周苏木精－伊红染色结果表明，实验组腱吻合口被胶原纤维连接，腱周及吻合口周围胶原纤维、成纤维细胞及肉芽组织明显较对照组少，腱周纤维组织较对照组粘连轻，粘连带稀少。

RESULTS: Wound healing rate, wound healing time, histopathology analysis, quantity assay of macrophage, determination of hydroxyproline, proliferation of cell, assay of DNA contents and circle of cells, level of transforming growth factor-alpha, levels of interleukin-1, interleukin-6 and tumor necrosis factor, assay of keratinocyte collagenase-1, level of fibroblast growth factor receptor-1, level of monocyte chemoattractant protein-1 and level of keratinocyte plasminogen activator inhabitor type 2 were selected as the evaluation criteria of wound healing.

结果 筛选了创面愈合率、创面愈合时间、组织病理学分析、巨噬细胞定量分析、羟脯氨酸含量测定、细胞增殖情况、细胞DNA含量和细胞周期分析、转化生长因子-α水平、白细胞介素-1、白细胞介素-6和肿瘤坏死因子水平、角质细胞胶原酶-1含量测定、成纤维细胞生长因子受体-1水平、单核细胞化学诱导蛋白-1水平和角质细胞纤溶酶原活化抑制剂-2水平等十三种创面愈合评价指标。

This study was conducted to examiune the fibrotic effect of Ni-Ti and 317L al loys in esophagus.The extract fluid from Ni-Ti,317L alloys was made according t o the ASTM standards of U.S.A. The Fb of esophageal scar was cultured primarily ,then incubated with alloy abstract fluid. The proliferating activity of Fb was measured by MTT at 4, 24, 48, 72 hours in the course of culturing. The esophagu s embedding test of Ni-Ti,317L alloys was made according to ASTM standards of U .S.A.The tissue around the alloys was taken at weeks 2 and 12,and the pathologi c changes were analysed.

为探讨新型支架材料Ni-Ti、317L合金在食管局部的致纤维化作用，按美国ASTM标准制备NiTi、317L合金的金属浸提液；&组织块培养法&原代培养食管壁疤痕的成纤维细胞，传代后以金属浸提液进行培养，分组后分别培养4、24、48、72 h，MTT法检测不同培养时间后Fb增殖功能的变化；按美国ASTM标准进行NiTi、317L合金试件的食管壁内包埋实验，即将金属试件经表面处理后直接置入食管壁粘膜层与肌层之间，术后2、12周取出包埋组织，分析试件周围组织的病理变化，并进行胶原纤维染色，观察纤维形成状况。

In contrast, hepatocyte growth factor has shown therapeutic effects on injured renal tubules in animal models.

此项研究中，采用人肾脏成纤维细胞培养系统，探讨肝细胞生长因子对高糖诱导的肾间质成纤维细胞损伤产生的作用。

Restriction mechanism for fiber elongation of Lil mutant grown in Held There were abnormities of ultra structure in 9 DPA fibers of Lil mutant whencompared with wild type: thinner cytoplasm, less functional organelles such as Golgi body and endoplasmic reticulum, ect, and more starch grains.

在田间条件下突变体纤维伸长受抑制的原因与野生型比较，突变体开花9d后的纤维细胞在细胞形态学上存在异常：细胞质染色较浅，各种功能性细胞器如高尔基体、内质网等少，有较多的淀粉粒等。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。