纤维细胞

They can increase the body's microcirculating blood, activate the histiocyte, effectively regulate the nervous system, unclog the meridian, enable the body to produce heating effects and improve sleeping quality; Moreover, the negative ion density of the bamboo fiber is up to 6000 per cubic centimeter, equal to the negative ion density content of the suburban fields, making people feel refreshing and comfortable.

竹纤维含有多种人体必需的氨基酸，-皮肤具有独特的保健功能；竹纤维素-竹密、果胶具有滋润皮肤和抗疲劳的-功效；竹纤维不带自由电荷，抗静电-止瘙痒；竹纤维制品质地柔软，亲和-肤，能改善人体的微循环血流，激活-组织细胞，有效调节神经系统，疏通-络，使人体产生温热效应，改善睡眠-量，此外，竹纤维中负离子浓度高达- 6000个/立方厘米，相当于郊外田野的负-离子浓度含量，使人倍感清新舒适，-以说是"家中相伴，在绿色竹林中徜-每一天"。

The cardiomyocyte viability were detected by MTT assay for calculating the survival rate and the inhibitory rate of the virus. Myocardial F-actin and VEGF were analyzed by immunofluorescent staining with confocal microscopy. Mean fluorescent intensity of F-actin and VEGF were determined by flow cytometry.Results There is no toxicity on cardiomyocytes when PD is at concentration of 0.02 mmol/L and 0.2 mmol/L while there is toxicity at concentration of 2 mmol/L. CVB3 could reduce the viability of cardiomyocytes, depolymerize F-actin cytoskeleton and reorganize VEGF protein.

体外培养心肌细胞，用100 TCID50柯萨奇B3病毒（CVB3）感染心肌细胞，建立病毒性心肌炎实验模型，然后分别用0.02mmol/L、0.2mmol/L、2mmol/L三种不同浓度的PD处理感染CVB3病毒的心肌细胞，观察心肌细胞的形态和搏动，用细胞免疫荧光技术标记纤维状肌动蛋白和VEGF蛋白，四唑蓝比色法测定心肌细胞活性和病毒抑制率，流式细胞仪检测各组心肌细胞VEGF蛋白和F-actin平均荧光强度。

Results All the 7 cases were female, 2 cases were solitary fibrous tumor, 2 cases were myofibroblastoma, 1 case was inflammatory myofibroblastic tumour, 1 case was fibromatosis, and 1 case was low grade myofibroblastic sarcoma.

结果 7例患者均为女性，2例为孤立性纤维性肿瘤；2例为肌纤维母细胞瘤；1例为纤维瘤病；1例为炎性肌纤维母细胞瘤；1例为低度恶性肌纤维母细胞肉瘤。

Methods Nuclear β-catenin expression was detected by immunohistochemistry in 77 lesions with desmoid-type fibromatosis and 171 other spindle cell lesions, including superficial fibrmoatosis (n=18), nodular fasciitis (n=36), keloid (n=16), scar (n=10), granulation tissue (n=9), synovial sarcoma (n=38), neufibroma (n=13), solitary fibrous tumor (n=12), gastrointestinal stromal tumor (n=10), low-grade myxofibrosarcoma (n=3), low-grade fibromyxoid sarcoma (n=3), and smooth muscle tumor (n=10). In addition, the immunohistochemical expressions of ER-α, ER-β and Ki-67 were examined in all of the lesions with desmoid-type fibromatosis. The nuclear immunohistochemical staining for nuclear βcatenin and ER-β was graded as high level (≥25% of cells), low level (5%-25%) or none.

采用免疫组织化学染色法检测77例韧带样型纤维瘤病和171例其它良、恶性梭形细胞病变（包括结节性筋膜炎36例、浅部纤维瘤病11例、瘢痕疙瘩16例、增生性瘢痕10例、肉芽组织9例、滑膜肉瘤38例、神经纤维瘤13例、孤立性纤维性肿瘤12例、胃肠间质肿瘤10例、低度恶性黏液纤维肉瘤3例、低度恶性纤维黏液样肉瘤3例及平滑肌肿瘤10例）组织中β-catenin核阳性的表达，同时检测韧带样型纤维瘤病中ER-α、ER-β和Ki-67的表达。

Consistent findings in lung biopsies of AP patients included endothelialitis of pulmonary vessels, active fibrosis, lymphangitic inflammatory infiltrates rich in plasma cells and histiocytes with or without nodule formation, and fibrinous pleuritis.

这6例肺穿的其他共同特征包括肺血管内皮炎、纤维化、淋巴管炎细胞浸润（其内富于浆细胞和组织细胞，伴或不伴淋巴滤泡形成）、纤维素性胸膜炎。

There were 22 malignant tumors including 11 osteosarcomas, 5 chondrosarcomas, 2 malignant fibrous histocytomas, and one each of malignant osteoblastoma, alveolar sarcoma, rhabdomyosarcoma, malignant giant cell tumor of bone. 28 benign tumors included 27 giant cell tumors of bone and 1 benign fibrous histocytoma.

肿瘤类型：恶性肿瘤22例，其中骨肉瘤11例，软骨肉瘤5例，恶性纤维组织细胞瘤2例，恶性骨母细胞瘤、腺泡状肉瘤、横纹肌肉瘤、骨巨细胞肉瘤各1例；良性肿瘤28例，其中骨巨细胞瘤27例，良性纤维组织细胞瘤1例。

The connection with other nucleis of the pallial thickening were studied by retrograde tracer horseradish peroxidase and fluorescent tracer dextran tetramethylrhodamine, Fluoro-ruby.

本研究采用Nissl染色法、golgi -cox法、神经元逆行追踪标记辣根过氧化物和逆行荧光染料葡聚糖四甲基罗丹明方法，分析了蛤蚧皮质加厚区的核团长度、核团体积、细胞大小、细胞形态、细胞密度、初步的亚区分区以及核团的纤维联系，为阐明爬行动物-蛤蚧的视觉通路核团的特征和纤维联系积累形态学和组织学资料。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400

图1 SHEE培养在盖坡片上，活细胞单层生长，胞浆较丰富，细胞核大小一致，有核仁×400 图2 SHEE培养细胞细胞核椭圆形，核仁较大，胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状，有伪足贴壁，表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁，呈星状或多角形，有丰富伪足和胞浆突，核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞，胞核和胞浆PI染色呈红色或淡红色，蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞，染色质凝集呈块状，右上角有一固缩细胞核TdT标记×400

2The histopathology of TMJ of PA rats showed pronounced proliferation of the synovium cells, some lytic collagenous fibers and degenerative chondrocytes in the condyles, and exudated protein and desquamating cells in the articular cavities with some fibrinous adhesions.

大鼠PA时，双侧TMJ均可见滑膜细胞显著增生；髁突表层胶元纤维松解，软骨细胞变性；关节腔可见蛋白渗出，细胞脱落，局部呈纤维性粘连。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。