纤维细胞

Objective To search for the best approaches to transfect plasmid pEGFP-N1 into human fibroblasts.

目的 采用3种不同的方法将质粒pEGFP-N1转染人成纤维细胞，从中寻找最佳的稳定转染的方法。

Results: it was efficient to transfect chicken embryonic fibroblast cells by the method of lipofectamine mediation. the expression levels of protein were significantly different.

结果 脂质体介导的方法能有效转染鸡胚胎成纤维细胞，实验中egfp蛋白相对表达量随质粒量的增加而明显升高（p.01）。

Normal human fibroblast spheroids were self-assembled and cultured for 1, 4, or 7 days, then combined in trough shaped recesses. Over a 24-h period the spheroids fused to become a rod shaped microtissue and the kinetics and extent of fusion could be quantified by assessing rod contraction.

正常的人成纤维细胞可自我聚集形成球体，并培养了1、4、7天，然后在槽状的凹处中进行结合。24小时时间段过后，球体融合成柱状的微组织，融合的动力学及程度可以通过评定这种小柱的收缩而进行定量分析。

Transmission electron microscopy demonstrated that fibroblast was full of endoplasmic reticulums that cisternal-like expanded in the rhEGF nanoparticle group, and collagen proteins were synthesized in cavity.

透射电镜下可见rhEGF纳米微球组成纤维细胞胞质内有大量的内质网，扩张成池状，腔内充满了合成的胶原蛋白。

AIM: To observe the influence of recombinant human relaxin on cardiac fibroblast functions under angiotensin II.

目的： 观察在重组人松弛素及血管紧张素Ⅱ作用下对心脏成纤维细胞功能的影响。

Restrain free radicels, fight against ageing, reserve fiber cell moisture and effectively reduce wrinkle.

抑制游离基、预防衰老，锁定纤维细胞中的水份，有效减退皱纹。

The recombinant fowl poxvirus rFPV-E0-E2 was serially passaged for 20 passages in specific-pathogen-free chicken embryo fibroblast culture. The passage 5, 10, 15 and 20 were chosen to identify by blue plaque assay, PCR amplification, gene sequence analysis and indirect immunofluoresence assay.

将重组鸡痘病毒rFPV-E0-E2在鸡胚成纤维细胞上连续传20代，取第5、10、15和20代重组病毒进行以下检测：用蓝斑试验鉴定各代重组病毒纯度；用PCR方法扩增猪瘟病毒E0、E2基因，进行基因序列分析；用间接免疫荧光实验检测各代重组病毒中猪瘟病毒E0、E2基因的表达。

"Second, both solid tumors and ascular anomalies (e.g., hemangiomas, port-wine stains) are known to hae increased expression of angiogenic proteins, including ascular endothelial growth factor and basic fibroblast growth factor."

&第二，已知实体瘤和脉管异常（例如血管瘤、葡萄酒色痣）存在血管原的蛋白表达增加，包括血管内皮生长因子和碱性成纤维细胞生长因子。&

Low levels of transcripts were also detected in other organs as well as mutant ovules by RT-PCR and Northern hybridization.

进一步的分析表明GhABC1基因在纤维细胞中的表达量很高，在子叶、叶片、茎等组织中低水平表达，在根中则检测不到其转录本。

The difference between the motion of the fish and the otoliths stimulate cilia on the sensory hair cells that are located in the inner ear.

鱼身体与耳骨反应动作的差异刺激了内耳感觉纤维细胞组织。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。