纤维细胞

Methods Hepatic cells were obtained from fetal rats by means of mechanical separation and hanging-drop culture, and after two days of primary culture, the medium was changed for further cell culture in the presence of 20% fetal bovine serum (containing glucose 25ml/L, mycillin 100 U/ml, pH 7.6), 10 mmol/L nicotinamide, 1 mg/L insulin, affix N2, basic fibroblast growth factor, stem cell factor, epidermal growth factor and leukemia inhibitory factor.

采用机械分离法结合悬滴培养法获得胎肝干细胞，原代培养2d后改变培养基，添加含20%胎牛血清的DMEM（含25ml/L葡萄糖，100U/ml青链霉素，pH值7.6），添加烟酰胺10mmol/L，胰岛素1mg/L，N2添加剂，碱性成纤维细胞生长因子、表皮生长因子、干细胞因子、白血病抑制因子各20μg/L诱导分化为nestin阳性细胞。

Fluid nitrogen cryopreservation has no obvious effect on the MHC antigeniacity of aortic homografts of rats.

液氮低温保存对大鼠主动脉平滑肌细胞和成纤维细胞MHC类的抗原性没有明显的影响。

In the rat carotid injury model, SM16 dosed once daily orally at 15 or 30 mg/kg SM16 for 14 days caused significant inhibition of neointimal thickening and lumenal narrowing. SM16 also prevented induction of adventitial smooth muscle alpha-actin-positive myofibroblasts and the production of intimal collagen, but did not decrease the percentage of proliferative cells.

在大鼠颈动脉损伤模型中，每日口服15或30mg/kgSM16持续14天能够显著抑制新生内膜增厚及管腔狭窄，预防对外膜α-SMA阳性肌成纤维细胞的诱导和内膜胶原的合成，但并不降低增殖细胞的比例。

BACKGROUND: Basic fibroblast growth factor can accelerate the bone marrow mesenchymal stem cells proliferation and differentiation into nerve cells, which is considered as a mitogen of glial cells.

背景：碱性成纤维细胞生长因子可以促进骨髓间充质干细胞的增殖和向神经细胞方向分化，并被认为是胶质细胞的分裂原。

Fibroblast growth factor is a kind of the polypeptide growth factors, which is known to be a mitogen for a wide variety of mesoderm-and neuroectoderm-derived cells and a neurotrophic factor for several neuronal cell types.

成纤维细胞生长因子是一种对中胚层及神经外胚层源的细胞都有促分裂活性的多肽生长因子，也是一种重要的神经营养因子。

Objective: To express recombinant human endostatin in yeast and study its mitogenic activity on 3T3 fibroblast cells.

摘 要 目的：利用酵母真核细胞表达系统表达出重组人内皮抑制素，并对其促成纤维细胞有丝分裂活性进行研究。

The multiplication conditions of bone marrow stromal cells were respectively detected by fibroblast colony forming in vitro, tritiated thymidine {[3H]TdR} incorporation and MTT.

分别应用成纤维细胞体外集落形成法、[3H]TdR掺入法和四甲基偶氮唑盐法检测骨髓基质细胞的增殖情况。

Methods The guts of embryonic mice removed and dissociated were plated into serum-free DMEM/F12 medium. The mitogen-free DMEM/F12 medium supplementd with 10% fetal bovine serum was used to induce differentiation of GNCSCs. Neurospheres and their derivations were determined with immunocytochemical and immunofluorescent staning.

分离胚鼠肠管，消化后接种于添加N2、B27和碱性成纤维细胞生长因子的DMEM/F12培养基，贴壁培养；连续传代后用血清促进GNCSCs分化；最后用免疫细胞化学方法染色鉴定。

Methods The guts of embryonic mice removed and dissociated were plated into serumfree DMEM/F12 medium. The mitogenfree DMEM/F12 medium supplementd with 10% fetal bovine serum was used to induce differentiation of GNCSCs. Neurospheres and their derivations were determined with immunocytochemical and immunofluorescent staning.

分离胚鼠肠管，消化后接种于添加N2、B27和碱性成纤维细胞生长因子的DMEM/F12培养基，贴壁培养；连续传代后用血清促进GNCSCs分化；最后用免疫细胞化学方法染色鉴定。

11 guinea pigs which were successfully induced myopias were extracted eyeballs, and the scleras near posterior poles were observed by immunohistochemistry methods. The positive rates of TGF-β〓 and bFGF in scleral fibroblast cytoplasma and the area percentageratio of positive cells were calculated and contrasted to controlled eyes.

将透镜诱导近视成功的11只豚鼠处死，摘除眼球，取后极部巩膜分别进行TGF-β〓和bFGF的免疫组织化学染色，观察它们在巩膜组织成纤维细胞胞浆中中表达的阳性率和阳性细胞所占面积的百分比，并与对照眼进行比较。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。