纤维细胞

Objective: Mast cell tryptase has been implicated in regulation of skin fibroblast, lung fibroblast and hepatic stellate cell proliferation and collagen synthesis.

目的：肥大细胞类胰蛋白酶已被证明在皮肤、肺成纤维细胞和肝星型细胞增生和胶原合成中具有重要作用。

The transformed cell could be agglutinated by lower concentration of ConA and could grow in semisolid agar.

为了探讨三氧化二镍(Ni2O3)诱导人肺成纤维细胞恶性转化作用以及β-胡萝卜素和维生素C的保护作用，用不同浓度的Ni2O3在体外多次处理HLF细胞，利用刀豆凝集素A凝集实验和软琼脂集落形成实验进行转化细胞的恶性鉴定；同时添加β-胡萝卜素(5.4mg/L)和维生素C的食物(1.8mg/L)，观察对Ni2O3转化作用的影响。

The results showed that 4-5 weeks after infection, only minimal granulomas were observed which consisted largely of eosinophils and lymphocytes.

结果发现，在感染后4～5周的未成熟虫卵周围，主要组成细胞是嗜酸性白细胞，其次是淋巴细胞、巨噬细胞和成纤维细胞。

After natural purification and three passaging, we detected CD90 expression in fibroblasts grown on cover glass by SABC immuocytochemistry techniques.

经过3次传代、自然纯化后得到性质稳定的成纤维细胞，冻存后复苏传1-3代，制成细胞爬片，免疫细胞化学SABC法染色检测CD90的表达情况。5。

Methods Human and pig dermal fibroblasts were isolated from the skin by enzymation and cultured in vitro. The second passage of human and pig dermal fibroblasts were collected and seeded at a density of 1.0×10^6 cells/mL onto cylinder-shaped polyglycolic acid scaffolds.

酶消化法获取人与猪皮肤成纤维细胞，培养扩增，取第2代细胞，以1×10^6/mL的细胞密度接种至聚羟基乙酸圆柱形支架上体外培养。

Methods: Myoblasts isolated from rat muscle samples were cultured and purified clonally in vitro. The 3rd passage cells were incubated with medium including basic fibroblast growth factor and dimethyl sulfoxid.

体外分离培养大鼠成肌细胞并克隆纯化，培养至第3代时加入含有碱性成纤维细胞生长因子和二甲基亚砜的培养液诱导分化，进行RT-PCR分析和免疫荧光细胞化学染色鉴定。

the possible mechanism of antiaging effect of fructus corni polysaccharides is to regulate the expression of cyclind1 and cdk4 in hdf senescence model cell.

山茱萸多糖可能通过改变细胞周期调控因子的表达而发挥其抗hdf细胞衰老作用。山茱萸多糖；cyclind1；cdk4；人二倍体成纤维细胞

After 3-8 passages, the oral CAFs and the normal mucosa fibroblasts from tissue culture were observed under light microscope. Vimentin,α-SMA and Cytokeratin protein were detected by immunohistochemistry SP method on glass coverslips. VEGF, TGF-β1, MMP-9, Tenascin-C mRNA were analyzed by semi-quantitative RT-PCR.

用组织块培养法获得原代CAFs和正常粘膜成纤维细胞，0.25%胰蛋白酶消化传代和纯化细胞；通过形态学观察和免疫细胞化学染色对口腔CAFs和NMFs进行鉴定：采用半定量RT-PCR检测口腔CAFs和NMFs 4种基因的表达。

Results:CKH,CK8 and CK19 were positive in spindle-shaped and polygonal epithlium tumor cells.Vimentin was positive in mesenchymal fibrocytes and smooth muscle actin in some mesenchymal cells.

结果：免疫组化标记表明，肿瘤上皮中梭形、多边形细胞CKH、CK8、CK19呈不同程度阳性，间质中纤维细胞vimentin阳性，部分间质细胞smooth muscle actin阳性。

Reverse transcription polymerase chain reaction and western blot were used to detect the COCH and POU3F4/brn4 gene's expression. To investigate in vitro characteristics of COCH gene's expession, two factors were introduced in the culture medium of spiral ligament type Ⅰ fibrocytes respectively. Firstly, phosphorothionate-modified antisense oligodeoxynucleiotides to POU3F4/brn4 mRNA was applied to study the regulation effects of POU3F4/brn4 to COCH gene transcription.

引入人工合成的针对POU3F4／brn4基因mRNA的反义硫代磷酸寡聚脱氧核苷酸，干扰耳蜗螺旋韧带Ⅰ型成纤维细胞POU3F4／brn4的表达，免疫印迹检测反义干扰细胞POU3F4／brn4蛋白条带的强弱，RT-PCR检测反义寡核苷酸干扰细胞COCH基因转录水平的变化，观察POU3F4／brn4对COCH基因的转录调控作用。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。