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纤维细胞

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In addition, after stably expression of bFGF, the biological characteristics of BMSCs and bone marrow-derived osteoblasts were analyzed in the fields of morphology, proliferation, cell cycle, alkaline phosphatase, typeⅠ collagen and so on.

从形态、增殖特性和细胞周期、碱性磷酸酶、Ⅰ型胶原等方面分析稳定表达碱性成纤维细胞生长因子的骨髓间充质干细胞和骨髓源性成骨细胞生物学特性。

Objective To study the influence of basic fibroblast growth factor on the growth of bone marrow stromal cells.

目的 研究碱性成纤维细胞生长因子对骨髓基质细胞体外定向诱导分化时细胞增殖的影响。

RESULTS AND CONCLUSION: The effect of different cells in epithelial tissue is known to all.

结果与结论:①上皮组织中各类上皮细胞的生理作用是明确已知的,其中研究最多的是角质形成细胞和成纤维细胞,并且与瘢痕形成密切相关。

METHODS By means of cell culture and flow cytometry we studied the effect of fluid nitrogen cryopreservation on the expres sion of MHC Ⅰ antigen of smooth muscle cells and fibroblasts of aortic homografts of ra ts.

采用细胞培养技术和流式细胞仪技术研究液氮低温保存对大鼠主动脉平滑肌细胞和成纤维细胞主要组织相容性抗原Ⅰ类表达的影响。

Basic fibroblast growth factor ; lymphokine activated killer cells ; cell division ; interleukin 2; bladder neoplasms ; immunologic cytotoxicity

碱性成纤维细胞生长因子;淋巴因子激活的杀伤细胞;细胞分裂;白细胞介素-2;膀胱癌;免疫细胞毒性

RESULTS: Positive immunohistochemical signals of MMPs and TIMPs could be found in fetal skin at different gestational periods.

结果 MMP -2、MMP -3、MMP -9、TIMP -1和TIMP -2在不同时期的胎儿皮肤内均有表达,它们主要分布于表皮细胞、成纤维细胞、毛囊和汗腺上皮细胞以及血管内皮细胞的胞浆中。

Mouse fetal tissues could be cut into uniform small pieces by scissors cutting and needle grinding, which grew well in tissue culture and great amounts of cells grew from the attached small tissue clump to form primary mouse embryonic fibroblasts monolayer.

将这些组织块培养48h,大量的梭形细胞从贴壁小组织块的边缘游离出的,细胞密度大,细胞生长旺盛,很快形成原代小鼠胎儿成纤维细胞单层。

Pue group is more formation of cartilage cells,cartilage cells,necrotic degeneration of the surface than the model group and the Sodium Chloride group with light rare exudation of inflammatory cells by microscope,we could see new blood vessels and fibroblasts.

光镜观察见葛根素组软骨细胞较平整,表层软骨细胞坏死变性较模型组和生理盐水组轻,炎性细胞渗出物少见,可见新生血管和成纤维细胞增生。

Methods The 3-5 generation fibroblasts were inoculated in 24-well with rat-tail collagen. A denuded area was made when the 3 generation of the fibroblasts was influent into a monolayer. Cells were then exposed to the presence of various concentrations of viper venom, mitomycin C, and the cells migrated into the denuded area were counted evely 6 hours.

将培养的第3~5代人结膜下成纤维细胞接种于涂有鼠尾胶原的24孔板中,待细胞生长融合后在培养板上划线,造成无细胞的裸露区,分别加人不同浓度的蛇毒制剂、丝裂霉素C及二者的混合液,每6h观察记录移行至裸露区的细胞。

In the present study, CHL cells were cultured routinely in MEM medium for 3 days. Then the cells were treated with sham exposure , 0.4mT 50 Hz sinusoidal MF , 0.4mT noise MF and the combined noise and 50Hz MF for 3 min and 15min, respectively. After exposure, the cells were lysed, and the proteins were extracted.

实验中,将中国仓鼠肺成纤维细胞分别经0.4mT工频磁场、等强度的噪声磁场、工频磁场与噪声磁场的复合磁场辐照及假辐照处理3min和15min后,裂解细胞,提取细胞蛋白质,以Western印迹技术,测定并比较细胞内SAPK磷酸化的变化。

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