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纤维细胞

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The inflammation reaction hyperplactic stage and lymphatic nodular stage are of nonspecific morphological changes and should be diagnosed with the help of positive tuberculin test and obvious increase of adenosine deaninase ; tuberculous nodular stage has a great number of epithelioid cells,and Langhans cells, caseous necrosis stage is characterized by a great deal of necrotic tissue and debris,a small number of fragmented epithelioid cells, and mainly by antiacid bacteria fibrinous hyperplastic stage is featured by a few fibrous tissue, cells and mucous oweing to hardness to get puncture, which neans tuberculous restoration,and scar formation.

炎性增殖反应期非特异性形态学变化,需要结合结核抗体阳性和腺苷酸脱氨酶明显增高有助于诊断,结核结节期主要可有较多类上皮样细胞及郎罕氏细胞;而干酪样脓样坏死主要见大量坏死组织及碎屑、少数残碎不全类上皮样细胞,此期主要能查到抗酸菌为特征;纤维增殖期,抽出物难取,仅见少数纤维组织、纤维细胞和黏液间质为其特征,提示结核恢复、瘢痕形成所致。

The inflammation reaction hyperplactic stage and lymphatic nodular stage are of nonspecific morphological changes and should be diagnosed with the help of positive tuberculin test and obvious increase of adenosine deaninase ; tuberculous nodular stage has a great number of epithelioid cells,and Langhans cells,caseous necrosis stage is characterized by a great deal of necrotic tissue and debris,a small number of fragmented epithelioid cells,and mainly by antiacid bacteria fibrinous hyperplastic stage is featured by a few fibrous tissue,cells and mucous oweing to hardness to get puncture,which neans tuberculous restoration,and scar formation.

炎性增殖反应期非特异性形态学变化,需要结合结核抗体阳性和腺苷酸脱氨酶明显增高有助于诊断,结核结节期主要可有较类上皮样细胞及郎罕氏细胞;而干酪样脓样坏死主要见量坏死组织及碎屑、少残碎不全类上皮样细胞,此期主要能查到抗酸菌特点;纤维增殖期,抽出物难取,仅见少数纤维组织、纤维细胞和黏液间质为其特征,提示结核恢复、瘢痕形成所致。

The inflammation reaction hyperplactic stage and lymphatic nodular stage are of nonspecific morphological changes and should be diagnosed with the help of positive tuberculin test and obvious increase of adenosine deaninase ; tuberculous nodular stage has a great number of epithelioid cells,and Langhans cells, caseous necrosis stage is characterized by a great deal of necrotic tissue and debris,a small number of fragmented epithelioid cells, and mainly by antiacid bacteria fibrinous hyperplastic stage is featured by a few fibrous tissue, cells and mucous oweing to hardness to get puncture, which neans tuberculous restoration,and scar formation.

炎性增殖反应期非特异性形态学变化,需要结合结核抗体阳性和腺苷酸脱氨酶明显增高有助于诊断,结核结节期主要可有较多类上皮样细胞及郎罕氏细胞;而干酪样脓样坏死主要见大量坏死组织及碎屑、少数残碎不全类上皮样细胞,此期主要能查到抗酸菌为特点;纤维增殖期,抽出物难取,仅见少数纤维组织、纤维细胞和黏液间质为其特征,提示结核恢复、瘢痕形成所致。

The inflammation reaction hyperplactic stage and lymphatic nodular stage are of nonspecific morphological changes and should be diagnosed with the help of positive tuberculin test and obvious increase of adenosine deaninase ; tuberculous nodular stage has a great number of epithelioid cells,and Langhans cells, caseous necrosis stage is characterized by a great deal of necrotic tissue and debris,a small number of fragmented epithelioid cells, and mainly by antiacid bacteria fibrinous hyperplastic stage is featured by a few fibrous tissue, cells and mucous oweing to hardness to get puncture, neans tuberculous restoration,and scar formation.

结核初期-炎性增殖期60例,占5.5%;结核早期-淋巴结节期130例,占11.9%;结核中期-结核性结节期有590例,占54.1%;结核晚期-干酪样脓样坏死期有280例,占25.7%;结核恢复期-纤维素增殖期30例,占2.8%。炎性增殖反应期非特异性形态学变化,需要结合结核抗体阳性和腺苷酸脱氨酶明显增高有助于诊断,结核结节期主要可有较多类上皮样细胞及郎罕氏细胞;而干酪样脓样坏死主要见大量坏死组织及碎屑、少数残碎不全类上皮样细胞,此期主要能查到抗酸菌为特点;纤维增殖期,抽出物难取,仅见少数纤维组织、纤维细胞和黏液间质为其特征,提示结核恢复、瘢痕形成所致。

The inflammation reaction hyperplactic stage and lymphatic nodular stage are of nonspecific morphological changes and should be diagnosed with the help of positive tuberculin test and obvious increase of adenosine deaninase ; tuberculous nodular stage has a great number of epithelioid cells,and Langhans cells, caseous necrosis stage is characterized by a great deal of necrotic tissue and debris,a small number of fragmented epithelioid cells

炎性增殖反应期非特异性形态学变化,需要结合结核抗体阳性和腺苷酸脱氨酶明显增高有助于诊断,结核结节期主要可有较多类上皮样细胞及郎罕氏细胞;而干酪样脓样坏死主要见大量坏死组织及碎屑、少数残碎不全类上皮样细胞,此期主要能查到抗酸菌为特点;纤维增殖期,抽出物难取,仅见少数纤维组织、纤维细胞和黏液间质为其特征,提示结核恢复、瘢痕形成所致。

Results: The study showed that the earliest stromal nodules were fibrous, and with the dual differentiation of myofibroblasts toward both smooth muscle cells and fibrous cells, they developed into fibromyomatous nodules and muscular nodules. The results of reclassifying the 55 nodules immunohistochemically were that 23(42%) was fibrous, 28(51%) fibromuscular nodules and 4(7%) muscular.

结果:结合HE切片和免疫组化结果发现前列腺间质结节的早期为幼稚的纤维性结节,随着结节内肌纤维母细胞向平滑肌细胞和纤维细胞的双向分化,间质结节可继续演化为纤维肌性结节和肌性结节。55个结节按免疫组化分类,纤维性结节占42%,纤维肌性结节占51%,肌性结节占7%。

Based on traditional wood fiber processing way at home and abroad, this paper draws up mathematical description theory of wood fiber, finishes the mathematical description theory of single wood fiber, sum up the reason of low density of wood fiber at microcosmos state, completed the laboratory study on Micron Flake Fiber High Strength Board, set up the forming methodology of this kind of wood-based panel.

本文在总结国内外传统法木纤维加工的基础上提出了木纤维数学描述理论,进行了单个木纤维细胞的数学描述,总结了现在木纤维强度低的微观原因,完成了微米长薄片木纤维高强度人造板的实验室研究,创立了该板种的形成方法学,所提出的MFB形成理论体系和加工方式,是利用木材微纳米技术在人造板加工业中开创出的一片新研究领域,提出了微米纤维细胞劈裂的条件。

Methods Pterygial samples were extracted and collected and the pterygial endothelial cells and pterygial fibroblasts were cultured alone, conditional and co-cultured to form different culture systems. The methods included that to select the suitable intensity of ultraviolet by MIT, to detect the changes of curves of growth about two kinds of cells by MIT and to explore the developments of protein and RNA of vascular endothelial growth factor and fibroblast growth factor-basic in three culture systems under ultraviolet whose intensity is 20 mJ/cm^2 by ELISA and RT-PCR.

收集翼状胬肉标本,采用血管内皮细胞和成纤维细胞单独培养、条件培养和共同培养的方法构建体系,用MTT法选择紫外线照射细胞的适宜强度;采用MTT法绘制强度20mJ/平方公分紫外线照射下细胞生长曲线;采用ELISA和RT-PCR检测紫外线照射下3种体系中细胞上清液和细胞中血管内皮细胞生长因子和成纤维细胞生长因子的蛋白和RNA含量变化。

A lot of organelles such as mitochondria, rough endoplasmic reticula and Golgi bodies were observed in fiber cell. Then, double karyotheca disappeared, and the organelles disintegrated. Multivesicular bodies appeared in fiber cytoplasm. With the further development of fiber, fiber wall underwent continual thickening with aging, and polylaminate structure gradually appeared. While the agglutinated nucleus, transfer vesicles, plasma membrane and plasmodesmata still presented.

研究发现,次生壁形成早期,细胞核具有双层核膜,染色质凝聚,可见大量的线粒体、粗面内质网和高尔基体等细胞器存在于纤维细胞中;随后,双层核膜消失,细胞器将逐渐解体,多泡体开始出现在纤维细胞的细胞质;随着年龄的增加,纤维细胞壁逐渐增厚,并出现多层结构现象,而运输小泡、细胞膜、胞间连丝和凝聚的染色质将持续存在。

Results: Compared with control group, apoptosis cells increased from 0.5%to 10%(some even to 15%) after 24,48 and 72 h action of -300,-500 and -1 000 V electrets. After action of -500 V PTFE electrets for 48-72 h, fibroblast cells showed characteristic morphological features of apoptosis. These features included chromatin aggregation, nuclear and cytoplasmic condensation and partition of cytoplasm and nucleus into membrane bound-vesicles.

结果:-300、-500和-1 000 V驻极体作用成纤维细胞24、48和72 h以后,与对照组相比,成纤维细胞的凋亡量从0.5%增至10%(部分可达15%);驻极体作用成纤维细胞48~72 h,出现细胞凋亡特有的形态学特征,即:细胞异染色质边集,细胞裂解,可见凋亡小体。

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