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纤维细胞

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In this report, immunohistochemistry and in situ hybridization techniques were perfected to examine the expression of bFGF, fibroblast growth factor receptor-I and bFGFmRNA of cultured HEP-2 laryngo-tumor cell and 803 gastro-tumor cell and of the solid tumor tissue formed by implanting the cultured cells into subcutis of nude mice, intending to elucidate the correlation between the expression level and the tumor formation.

本研究建立了免疫组化和原位杂交的检测方法,通过对体外培养的HEP-2喉癌细胞和803胃癌细胞和这两株细胞在裸鼠皮下形成的实体瘤组织中bFGF,纤维细胞生长因子受体-1(fibroblast growth factor receptor-1,FGFR-1)和bFGFmRNA的检测试图揭示三者在体外和体内表达以及与肿瘤形成的关系,从而为这方面的研究提供更多的材料。

The first passage of YS-MSCs which grew in good condition was obtained, and adipogenic differentiation of YS-MSCs was induced by dexamethasone and insulin, and angioblast was induced by vascular endothelial growth factor and basic fibroblast growth factor.

取生长状态良好的第1代细胞,以地塞米松、胰岛素定向诱导卵黄囊间充质干细胞向脂肪细胞方向分化;以血管内皮生长因子和碱性成纤维细胞生长因子体外诱导其向血管内皮细胞方向分化。

One eye in patients was selected to do the tear film breakup time, Schirmer test and cornea fluorescein staining, and the expression of NFκB and TGFβ1 on bulbar conjunctiva endepidermis was detected by immunohistochemistry.

TGFβ1是多功能细胞因子,对上皮细胞具有较明显的抑制作用,对成纤维细胞及其他间叶组织来源的细胞均具有明显的刺激作用,表现为胶原合成增加,有利于组织修复及伤口愈合[3,4]。

Results MMP-2 immunohistochemical results indicated that the MMP-2 expression increased and was observed on both sides periodontium of movement tooth, osteoclast, cementoblast, osteocyte, fibroblast and osteoblast appeared positive.

结果MMP-2免疫组化结果显示牙齿移动后,牙周膜两侧MMP-2表达增加,破骨细胞、骨细胞、成纤维细胞和部分成骨细胞MMP-2染色阳性。

Methods Epithelial cells and fibroblast from ameloblastoma, keratocyst, dentigerous cyst, radicular cyst and oral mucosa were cultured and observed by phase-contrast microscope.

原代体外培养成釉细胞瘤细胞、牙源性角化囊肿、根端囊肿及口腔粘膜的上皮细胞和成纤维细胞,倒置光相差显微镜观察。

Results In animal study, lumbricus could inhibit the growth of staphylococci, bacillus coli and bacillus aeruginosus. The time of wound healing in experimental group was 4 days shorter than that in control group. At 4d and 7 day the numbers of the capillary, blood vessel endodermis and desmohemoblast desmocyte and splitting epithelium of trial group were much more than those of control group. At 4d the trial group′s numbers of splitting mesenchymal cell were much more than that of control group. From 3d on the wound healing and granulation filling of experimental group were much quicker than those of control group.

结果 动物实验中,地龙能抑制金黄色葡萄球菌、大肠杆菌及绿脓杆菌的生长;实验组创面愈合时间较对照组提前了4天;第4、7天实验组动物创面的毛细血管数、血管腔内皮细胞数及间质成纤维细胞数均较对照组明显增多,上皮细胞分裂象也高于对照组,第4天的间皮细胞分裂象也高于对照组;自第3天始创面愈合及肉芽充填速度实验组明显快于对照组。

RESULTS:①The cells showed fusiform shape, reticulate at low density and fasciculate at high density under inverted phase contrast microscope. Mouse anti-Vimentin was positive.

结果:①巩膜成纤维细胞鉴定:倒置相差显微镜下观察,细胞透亮呈梭形,在细胞密度较低时细胞排列成网状,密度较高时呈束状;波形蛋白染色阳性。

The plasticity of NSCs was detected by the differentiating test. HESCs were induced into dopaminergic neurons by means of adding Sonic Hedgehog Peptide and fibroblast growth factor eight (FGF8) early under the condition of mocked microenvironment and different development stage of dopaminergic neurons in vivo ,meanwhile made comparison with traditional method . The marker of dopaminergic neurons was tested by immunocytochemistry and RT-PCR, the content of dopamine and its derivation homovanillic acid were measured by HPLC-ECD.

模拟体内多巴胺神经元分化发育的不同阶段及微环境,采用早期添加音猥因子及成纤维细胞生长因子-8(FGF8)的方法,诱导hESCs定向生成多巴胺能神经元,并与传统的诱导方法相比较,免疫荧光细胞化学和RT-PCR法检测多巴胺能神经元的标志,高效液相色谱法检测诱导后细胞及细胞培养上清液中多巴胺及其代谢产物高香草酸的含量。

Results ⑴ The cell proliferation was inhibited by TET. It showed thatdivision index and cell number during G0-G1 were decreased, while S and G2-M phases were lengthened with dosage and time dependency in dosages of TET from 2mg/L to 8mg/L; besides, oval-shap cells, karyopyknosis and fragmentation were observed under light microscope. The inhibitory effect in T2 group was statistically similar to that in triamcinolone.

结果⑴ TET在2mg/L~8mg/L之间呈剂量及时间依赖性地抑制瘢痕成纤维细胞的增殖,使生长曲线不同程度的压低、分裂指数降低、G0-G1期细胞数减少、G2-M期增多、S期与G2-M期延长,光镜下见细胞突起变短,细胞由梭形变成椭圆形,核固缩、碎裂。P.C的改变近似于T2组(4mg/L TET)。

Immunohistochemical staining revealed that expression of RANKL and OPG protein was detected in osteoblasts, bone lining cells, fibroblasts and osteoclasts which mostly located in resorption lacunae.

免疫组化结果显示,RANKL和OPG的阳性表达位于牙周成骨细胞、骨衬里细胞、牙周纤维细胞和位于骨陷窝内的破骨细胞中。

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