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纤维细胞

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At 12w after operation, the cells in control group still behaved as fibroblasts and fibrocartilage like cells were predominant in grafts of cell and gene groups , the grafts in these two groups were similar to normal ligament morphologically.

手术后12周,对照组显现移植物内有大量的成纤维细胞,甲苯胺兰染色轻度异染;而细胞组和基因组细胞量减少,显现类软骨性状,细胞排列与正常前交叉韧带接近。

Expression of hepatocyte markers such as ALB and CK18 was measured with immunohistochemistry. RESULTS: Cells in control group kept the long spindle shape, with shape agent as 4.5±1.0 and small size, proliferated quickly, submitted typical fibroblast whirlpool or flamboyancy growth. Five to seven branches were observed at the late culture period, no cell rounded, and the rate of dikaryocyte was less than 0.1%.

结果:未进行诱导的对照组细胞保持长梭型,形状因子4.5±1.0,面积较小,增生较快,呈典型成纤维细胞的漩涡状或火焰状生长,培养后期可见5~7个分支,无细胞变圆,双核细胞率小于0.1%,逐渐老化,颗粒增多。

At 18 and 24 hours after culture, types of cell death were quantitatively detected using fluorescein isothiocyanate and propidium iodide staining.

25%,50%,100%浓度的3种材料浸提液体外培养小鼠成纤维细胞L929 24 h后,采用流式细胞术碘化丙啶染色法检测L929细胞的凋亡率及细胞周期分布情况。

And, normal keratinization can be observed by scanning electron microscape on the surface of the PCS.

通过冻干法制备的蛋白改性壳聚糖多孔支架对细胞没有毒性,L929细胞和人成纤维细胞可以在支架表面和内部正常生长和增殖,角质细胞在材料表面可以正常老化、角质化。

The protoxylem vessels initiated lignification in the early stage of vascular bundle differentiation, whereas metaxylem vessels and fiber walls started lignification from the middle lamella and cell comers after completion of vascular bundle differentiation. The walls of most parenchyma cells lignified after the stem reached its full height, while a few parenchyma cells remained non-lignified even the culm is 7 years old.

研究结果表明:毛竹茎的原生木质部导管在维管束发育早期就已木质化;后生木质部导管和纤维细胞在维管束分化完成后,自胞间层和细胞角隅处开始木质化;基本薄壁组织细胞木质化的发生较晚,通常在茎的节间完成伸长生长后才开始,但也有少数薄壁组织细胞始终保持非木质化的薄壁状态。

RESULTS: Otocyst cells differentiated and proliferated and showed several morphotypes, mainly including epitheliallike cells, fibroblastlike and neuronlike cells.

结果:培养的细胞在体外进行分化和增殖,细胞形态主要有上皮细胞形态、成纤维细胞形态和神经细胞形态3种。

RESULTS摘要: Otocyst cells differentiated and proliferated and showed several morphotypes, mainly including epitheliallike cells, fibroblastlike and neuronlike cells.

结果摘要:培养的细胞在体外进行分化和增殖,细胞形态主要有上皮细胞形态、成纤维细胞形态和神经细胞形态3种。

The surface antigen expressions of MNC were detected by flow cytometry. MNC were induced with DMEM medium, containing HGF alone, FGF4 alone, HGF+FGF4 or no growth factor, respectively. The markers of hepatic linear cells were examined before induction, and 7, 14, 21 and 28 d after induction by RT-PCR, immunocytochemistry and periodic acid-schiff in every group.

分别用肝细胞生长因子、成纤维细胞生长因子-4(FGF4)、HGF+FGF4、无生长因子4种处理因素进行诱导培养,于诱导前及诱导后的第7、14、21、28天采用RT-PCR检测AFP、白蛋白及C-met、FGFR2 mRNA的表达,免疫细胞化学法检测肝细胞抗原和CK18的表达,PAS 法进行糖原染色。

To study the effect of basic fibroblast growth factor and nerve growth factor on immune function in mice. Methods: Through the assays of Phagocyte, PFC, MTT and ELISA, the action of Mφ, B lymphocyte and T lymphocyte of mice was observed.

细胞生长因子是多功能的生物活性多肽,包括成纤维细胞生长因子、神经生长因子和表皮生长因子等,作为在细胞间传递信息的物质,细胞生长因子在机体内发挥着极其广泛的生物学作用,参与多种病理生理过程,如生长、免疫、肿瘤、创伤愈合等。

To explore the potential mechanism of cigarette smoke extract -induced cell injury, primary cultured skin keratinocytes and fibroblasts from rat were selected as target cells in Part Ⅰ study compared with the effect of UVA.

本研究第一部分以原代培养的大鼠皮肤角质形成细胞和成纤维细胞为对象,采用UVA损伤作用为参照,探索香烟雾提取物(Cigarette SmokeExtract,CSE)对皮肤细胞的损伤作用,重点观察CSE所致的皮肤细胞毒作用、功能损伤及可能作用机制。

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