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纤维细胞

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EGF remarkably stimulated the dermal sheath fibroblasts, dermal Tibroblasts and demal papilla cells. Effection on the dermal sheath fibroblasts was the strongest. bFGF, GM-CSF, insulin and hydrocortisone had not effective on them.

EGF对真皮鞘细胞、真皮成纤维细胞和毛乳头细胞有显著的促生长作用,其中对真皮鞘细胞的作用最强,而bFGF、GM-CSF、胰岛素+氢化可的松对三种细胞没有明显的作用。

RESULTS: hADSCs presented fibroblast-like morphological feature with a flocked array. The 3rd passage of hADSCs had unique immunophenotypes and they were positive for CD73, CD44, CD166, CD105 and CD29, but negative for CD31, CD34, CD45 and HLA-DR. Cell cycle result showed that they had the typical growth characteristics of stem cells, namely, 83.81% cells stayed at G0/G1 stage, only 16.19% cells were stayed at S+G2/M stage; The latent phase of the primary culture cells was 2 days prior to and after incubation, followed by 3-6 days of logarithmical proliferation, reached a peak at day 6, and entering the growth platform phase with lower growth speed.

结果:分离培养的人脂肪间充质干细胞为成纤维细胞样,呈漩涡状排列。P3代人脂肪间充质干细胞呈CD73,CD105,CD166,CD44及CD29阳性,而CD31,CD34,CD45及HLA-DR阴性;具有典型的干细胞增殖特点,83.81%细胞处于G0/G1期,仅16.19%细胞处于S+G2/M期;细胞在接种后前2 d处于生长潜伏期,第3~6天处于对数生长期,第6天达峰值,以后细胞生长速度减慢,进入生长平台期,平均五六天传代1次。

These cell types are thin-walled cells, meristematic cells, pigment cells, fibroblasts and so on.

这些细胞类型有薄壁细胞、分生细胞、色素细胞、纤维细胞等等。

The therapeutic mechanism of the method of nourishing qi, activating blood, dispersing phlegm and promoting the collaterals was supposed as that it intervened the airway remodeling and alleviated the progress of airflow obstruction of COPD by suppressing the chemo taxis of cytokines, regulating the proliferation of fibroblast cell and smooth muscle cell and preventing the proliferation and plerosis of extracellular matrix.

推测益气活血化痰通络法防治COPD的机制可能为:通过抑制炎症细胞的趋化、影响细胞因子的活性、调节成纤维细胞和平滑肌细胞的增殖、阻止细胞外基质的增生修复,从而达到干预COPD患者气道结构重塑,减缓COPD气流受限进展的作用。

The COX-2 positive cells were odontoblasts, pulpal fibroblasts vascularendothelial cells and macrophages (positive neutrophils could be found in some cases of the second group and of the third group).(3)We found that COX-2 developed up with HIF-1 a in third group ,but against it infourth group.

COX-2表达阳性的细胞有成牙本质细胞、成纤维细胞、血管内皮细胞和单核巨噬细胞(阳性中性粒细胞可出现在第二组和第三组),HIF-1α与COX-2的表达相关主要在第三组和第四组牙髓标本中体现明显,在第三组二者的变化大致呈平行关系;在第四组基本呈反向变化关系。

After renal cortexes were Grinded and digested with 0.25%tryspase.The cell types of epithelial cell were identified by immunocytochemistry.The injury model of rats, renal tubular epithelial cells were established by using gentamicin.And observed the role of bFGF in renal tubular epithelial cell.

通过对大鼠肾皮质研磨、过网的方法,并以0.25%胰蛋白酶消化,以免疫细胞化学方法鉴定体外培养的肾小管上皮细胞(抗Cytokeratin 18抗体),以庆大霉素对培养的肾小管上皮细胞建立损害作用模型,然后观察碱性成纤维细胞生长因子对损害的肾小管上皮细胞的保护作用。

CD34+ cells were harvested from G-CSF mobilized adult blood and umbilical cord blood and incubated onto fibronectin/gelatin-coated Petric dishes in the presence of recombinant human vascular endothelial cell growth factorand basic fibroblast growth factor.

毛宁为证明出生后血液循环中存在内皮细胞的前体细胞,从G-CSF动员的成人外周血及脐带血中分离CD34+细胞,将之接种于纤连蛋白与明胶铺板的培养皿上,培养体系中加入重组人内皮细胞生长因子和重组人碱性成纤维细胞生长因子。

Specific inhibition of Hh signalling using small molecule inhibitors, a neutralizing anti-Hh antibody or genetic deletion of smoothened in the mouse stroma results in growth inhibition in xenograft tumour models.

由癌细胞分泌的Hedgehog配体,在肿瘤表皮细胞中未能激活信号作用,而是在&基质&上发挥作用(&基质&由细胞外基质、成纤维细胞、内皮细胞和微血管组成,恶性细胞着床于其中)。

The methods of sticking tissues piece to culture primary hFCECs and mouse 3T3 fibroblast conditioned medium to culture passage hFCECs were established;2.The Proliferative capacity of corneal limbus hFCECs is better than central hFCECs.3.3T3 conditioned medium could markedly promote the proliferation of epithelial cells

结论一、组织块贴壁法原代培养人胎儿角膜上皮细胞以及小鼠3T3成纤维细胞条件培养液传代培养方法基本建立;二、人胎儿角膜缘部上皮细胞增殖能力优于中央部;三、3T3细胞条件培养液能促进角膜上皮细胞的增殖。

He expression of TLR4 is also increased in the peripheral blood monocytes from the patients with ACS.

LR4在动脉粥样斑块的内皮细胞、巨噬细胞、血管平滑肌细胞和血管外膜成纤维细胞表达均增高,在急性冠脉综合征患者外周血单核细胞表达增高。

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