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纤维细胞

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It has several kinds of functions : It could accelerate blood coagulation; has chemotaxis to monocyte and neutrophil , could appeal to fibroblast and cartilage cell to move to the region of the injury, takes part in the signal transduction and activation in cell , and takes part in the growth and differentiation of cell and repair in trauma.

它的功能多样,能加速血液凝固,对单核细胞和中性粒细胞具有化学趋化作用,能吸引成纤维细胞和软骨细胞向损伤区域移动,参与细胞的信号传导与活化,细胞的生长及分化,创伤修复等一系列重要生理和病理过程。

Tumor associated protein n35 was located on the centriole of the chromosomal domain at the s-g2 phage.conclusions the tumor associated protein n35 might be a tumor cell growth regulator, expressed only it's the cancer cells and related with the proliferation of cancer cells.

结果 肿瘤相关蛋白n35不存在于正常人淋巴细胞、正常肺细胞及人皮肤纤维细胞中,而存在于肺癌细胞蛋白中,在亚细胞结构中主要分布于胞核,在肿瘤细胞有丝分裂进入g2至m期时明确地定位于中心粒结构上。

It distributed in the live lung cancer cell GLC-82, but not in the protein ingredient of the normal human lymphocyte, the normal human lung cell and human skin fibroblasts, Tumor associated protein N3S was located on the centriole of the chromosomal domain at the S-G2 phage.

结果:肿瘤相关蛋白N35不存在于正常肺细胞、人淋巴细胞及人皮肤纤维细胞中,而存在于肺癌细胞蛋白中,在亚细胞结构中主要分布于胞核,在肿瘤细胞有丝分裂进入S至G_2期时明确地定位于中心粒结构上。

Result The flexibility of the scaffold material was powerful. The load-elongation curve of the mechanical testing was similar to that of ACL, the maximum load, the ultimate stress and the Young's modulus of the scaffold materials were 52.61 N, 14.96 MPa and 202.08 MPa respectively. Human ACL cells displayed the representative characteristercs of the ligamentous fibroblasts, and synthesized extracellular matrix, such as type Ⅰand Ⅲ collagen protein. The scaffold materials had no cytotoxicity. Human ACL cells adhered, grew and proliferated well both on the surface and in the holes of the scaffold materials.

结果]韧带支架材料的柔韧性强,拉力测试的负荷-拉伸曲线与韧带的拉伸曲线相似,其最大负荷、极限应力和弹性模量分别为52.61N、14.96MPa和202.08MPa;体外分离培养的人前交叉韧带细胞呈典型的成纤维细胞特征,能在体外分泌Ⅰ、Ⅲ型胶原等细胞外基质;支架材料无细胞毒性,人前交叉韧带细胞可在支架材料上黏附、生长并分泌细胞外基质。

In order to study the mechanism of the inhibitory effect of salvia miltiorrhiza and tetramethyl pyrazine on scar fibroblast, the DNA content of fibroblast and the all distribution in cellular cycle was measured by FCM. The hypertrophic scar tissue of chest was chosen for primary culture of fibroblast.

摘 要 应用流式细胞光度分析法测定丹参和川芎嗪对体外培养的瘢痕成纤维细胞DNA相对含量和细胞周期时相变化,发现药物在一定浓度作用下,DNA指数无明显变化;丹参使C2-M期细胞分布增多,G2-M期延长;川芎嗪使G2-M期细胞分布增多,G2-M期、S期延长;药物使细胞群体倍增时间延长,与浓度呈明显直线正相关。

Results Most OECs were bipolar or tripolar, and the purity was estimated to be over 88.7%. The non-OECs cells consists of astrocytes, neurons, oligodendrocytes and fibroblas.

结果 体外培养的新生小鼠嗅鞘细胞主要为双极或三极细胞,其纯度可以达到88.7%以上,污染细胞中有星形胶质细胞、神经元、少突胶质细胞和成纤维细胞

The conclusions were that the inhibitory effect of SM was the result of inhibiting the mitosis of cells and the cellular cycle be at a standstill in G2-M stage.

应用流式细胞光度分析法测定丹参和川芎嗪对体外培养的瘢痕成纤维细胞DNA相对含量和细胞周期时相变化,发现药物在一定浓度作用下,DNA指数无明显变化;丹参使C2-M期细胞分布增多,C2-M期延长;川芎嗪使C2-M期细胞分布增多,C2-M期、S期延长;药物使细胞群体倍增时间延长,与浓度呈明显直线正相关。

With the increase of cell density, soma was slenderer and slenderer and like fibroblast.② Growth curves of PDMCs: Growth latency ranged from 2 to 8 days after cell inoculation. During this period, adherence was observed gradually but not obviously amplified. Eight days later, cells entered log growth phase.

结果:①多分化潜能细胞的原代培养情况:胎盘组织消化后获得细胞中仅有少量的贴壁细胞,经两周后逐渐形成扁平单层细胞,呈漩涡状生长或成簇生长,随着细胞密度的增加,胞体变得细长,形态类似成纤维细胞

Cell proliferation was measured with cell number,cell metabolic activity (WST-1 cleavage),DNA synthesis and cell cycle assayed with flow cytometry.

进行新西兰白兔心脏心肌成纤维细胞的培养;测定细胞计数,细胞活性(WST-1分解)和DNA合成,并以流式细胞仪测定细胞周期等以表示细胞增殖。

Methods: Cardiac myofibroblasts derived from heart of New Zealand rabbit were cultured and identified. Cell proliferation was measured with cell number,cell metabolic activity (WST-1 cleavage),DNA synthesis and cell cycle assayed with flow cytometry.

进行新西兰白兔心脏心肌成纤维细胞的培养;测定细胞计数,细胞活性(WST-1分解)和DNA合成,并以流式细胞仪测定细胞周期等以表示细胞增殖。

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