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纤维细胞

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We also confirmed in this paper that it is injected somatic cells but not broken cytoplasm formed multi blastomere through mitosis in the reconstructed embryos.

在本实验中还通过用DNA染料(Hoechst-33342)对核移植体进行染色的方法,对融合后成纤维细胞在核移植体内不同时间的运行及发育模式进行了观察,确认了是成纤维细胞经有丝分裂形成多个卵裂球而不是由于细胞质碎裂形成的两个或多个碎块。

Periostin may affect the function of fibroblasts of hyperplasic scars. periostin may be a cicatrix specific gene.

periostin在过度增生性瘢痕成纤维细胞中的表达增高,氢化可的松可能抑制其基因的表达;periostin可能影响过度增生性瘢痕成纤维细胞的功能,它可能是一种瘢痕相关基因。

MethodsPhotoaging skin fibroblast models were induced by 8-MOP/UVA. Cordyceps polysaccharides was administrated before 8-MOP/UVA .HE stained,MTT, hydroxyproline, MDA and SOD quantitant were used to test the effects of cordyceps polysaccharides. ResultsCell crimple,condensation of nuclear chromatin in 8-MOP/UVA model group were observed.

方法用8-甲氧补骨脂素联合UVA(8-MOP/UVA)诱导皮肤成纤维细胞光老化,用HE染色、MTT、羟脯氨酸含量、丙二醛含量检测及SOD活力分析等方法观察虫草多糖对成纤维细胞光老化过程中的形态、活力、胶原合成及抗氧化能力的影响。

After then, the phenol and croton oil chemical peeling agents were used to the UVA/B-irradiated skin of hairless mice and untreated group as controls.

同时本实验分别应用酚和巴豆油作用于体外培养原代成纤维细胞,检测成纤维细胞中前体胶原蛋白的表达。

Because different cell lines may show various levels of susceptibility to adenovirus infection and virus production, non-selectively replicating wtAd5 was used to be a comparison with the tested conditionally replicating adenovirus CNHK500.ONYX-015, the first replication-competent adenovirus entered into clinical trials was also used to be a comparison. We used virus proliferation assay, cell viability assay and western blot to evaluate the proliferation and cytolysis selectivity of CNHK500. Results demonstrated that the CNHK500 virus proliferation multiples in breast cancer cell lines after 48 hours is similar to that of wtAd5, ONYX-015 virus proliferation ability is less than that of CNHK500 in cancer cells.

然后,通过与ONYX-015(E1B 55 KD a蛋白缺失的2型和5型嵌和型腺病毒,美国ONYX生化制药公司研制)、wtAd5进行对比,利用病毒增殖实验和细胞生长抑制实验,验证了CNHK500选择性增殖能力和肿瘤特异性杀伤作用;利用Western Blot检测CNHK500腺病毒E1A和E1B在乳腺癌细胞和正常成纤维细胞中的表达差异,揭示了腺病毒肿瘤靶向性的机制;利用携带绿色荧光蛋白的CNHK500-EGFP感染乳腺癌细胞株和正常成纤维细胞株,直观地观察其感染乳腺癌细胞的能力和在乳腺癌细胞中的增殖过程。

GM-CSF had dual effects on the cell growth,it could inhibit the fibroblasts proliferation at concentration abov e 1mg/L while stimulate the cell growth at concentration between 1mg/L and 0 .001mg/L.

GM-CSF的浓度在0.001~1mg/L之间对牙周膜成纤维细胞、牙髓成纤维细胞有明显的增殖效应,大于1mg/L时对增殖有明显的抑制作用。

It's also where cells called fibroblasts produce collagen and elastin fibers.

它的细胞,也是所谓的成纤维细胞产生胶原蛋白和弹性蛋白纤维。

Adult male foreskin subcutaneous tissues apart from epidermis and hypodermis were taken by surgery operation and human foreskin fibroblasts were isolated and cultured, then enhanced green fluorescence protein in human fibroblasts were expressed by lipofectamine and lentiviral vectors, respectively.

取成年男性包皮皮肤的皮下组织,分离培养人皮肤成纤维细胞,分别采用脂质体和慢病毒载体介导转染人皮肤成纤维细胞表达绿色荧光蛋白。

Objective: To Compare the differences in the level of proliferation and apoptosis in keloid fibroblasts and normal skin fibroblasts.

目的:比较瘢痕疙瘩成纤维细胞与正常皮肤成纤维细胞在增殖-凋亡方面的差异。

An attenuated vaccine strain of Muscovy Duck Reovirus, which was suitable for propagating in chicken embryo fibroblast, was obtained by serial passage alternately between Muscovy duck embryo fibroblast and CEF.

应用番鸭胚成纤维细胞和鸡胚成纤维细胞交替传代选育出适应CEF繁殖的番鸭呼肠孤病毒弱毒疫苗株。

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