纤维组织原的

RESULTSdiabetic rats showed typical symptoms involving elevated serum glucose and weight loss (2)The rats in DM+VaD group reacted slower and made more error numbers in Y-maze than the rats in VaD group did, with a prolonged total reacting time in a whole day.(3)More neurons with serious damages, such as edema, ischemia or pyknosis could be found in DM+VaD group than in VaD group. More disarrangement of cone neurons, obvious glia proliferation, and more neuron apoptosis were found in CA area of hippocampus. CONCLUSIONS (1)2-VO in STZ-induced diabetic rats succeeded in establishing VaD animal models.(2) The cognitive dysfunction induced by VaD rats had been deteriorated by diabetes mellitus.(3)Morphology evidences proved that diabetes mellitus aggravated brain damages induced by VaD.PART II Effect of diabetes mellitus on the cholinergic nervous systemin CA1 area of hippocampus of VaDOBJECTIVE To estimate the role of cholinergic nervous system in hippocampus during the process of cognitive dysfunction aggravated by diabetes mellitus in DM+VaD group. METHODS Observe the changes of ChAT protein by immunohistochemistry stain. Chose three rats randomly from each group at each pointat two week, four week, and eight week, then sepa

经腹腔注射链脲佐菌素诱导慢性实验性糖尿病，1周后永久性结扎双侧颈总动脉(2-VO)制作VaD模型记录术后2周、4周和8周各组大鼠的体重及血糖；应用Y-迷宫检测空间定向学第二军医大学博士论文中…丈摘…要1ia；一色恤~﹁染澎糕行HE染色观察组织病理学孪叱；胶质细胞原纤维酸性蛋白protellZ，Cf冰尸标记观察星形胶质细舱的激活情况、橄声软公点娜育票票众糯黑橄居端粗默孺篇筑价井袱第二部分糖尿病对血管性痴呆大鼠海马c心区胆碱能神经系统的影响探讨海马CAI区胆碱能神经系统在糖尿病加重确D一大鼠认知障碍中所实验动物及分组同第一部分，应用免疫组织化学染色方法现察海马CAI{介狱牛第晕军l袭拔常博粗{论义

The histological findings of the primary tumor and metastatic lymph nodes demonstrated massive cancer cell degeneration such as pycnosis and vacuolation, xanthogranulomatous inflammation and dense fibrosis. The effect of NAC was judged to be grade 2 histologically.

组织学检查发现肿瘤原发灶和转移的淋巴结癌细胞大量退化如核固缩和空泡形成，黄肉芽肿炎以及密集的纤维化，经组织学判定NAC的疗效为2级。

In this context, we have reported recently that the volume of myocardial tissue occupied by perimysial and endomysial collagen was lower in hypertensive patients with HF and depressed ejection fraction, than in hypertensive patients with HF and preserved ejection fraction and normotensive subjects.56 Of interest, the interstitial and perivascular accumulation of collagen fibres was abnormally high in the two groups of patients.

在这种情况下，我们已经说了占用体积perimysial心肌组织人肌内膜胶原水平低的高血压患者用高频和沮丧的射血分数在高血压患者的心力衰竭和保存被罚出场分数与正常血压subjects.56舒张压的利息、间质和血管堆积过高的胶原纤维是在两组病人。

The ligamentous scaffold material were made from the PVA fibers by braiding technique. The mechanical properties of the scaffold materials were measured using the electronic materials testing machine. Human ACL cells were isolated and cultured by tissue explant and collagenase digestion methods, the morphous characteristics of cells and the secretion of collagen protein were measured. In order to observe the biocompatibility between the material and cells, ACL cells were in vitro expanded and seeded on the scaffold materials.

方法］用聚乙烯醇纺丝纤维编织构建韧带支架材料，在电子拉力机上测试该支架材料的力学性质；用组织块和胶原酶消化培养法在体外分离培养人前交叉韧带细胞并对细胞的生长形态和分泌胶原蛋白的特征进行检测，细胞经体外扩增后种植于编织构建的聚乙烯醇韧带支架材料上观察。

Under the conditio of pressure level 7000atm,duralion time 20 min an room temperature,samples had much different micrographical structures of myofibrils compared with contral groups by using Transmissin electron micrograph.

本文研究分析了高压处理后的牛羊肌肉的显微组织变化，经对压力为7000atm，施压介质温度为室温，施压持续时间为20min的处理样品和相应的对照组分别进行了透射电子显微镜观察分析其肌原纤维的显微组织变化。

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物；培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感，据此可在传代过程中将二者分离纯化，获得纯细胞系；混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞；再加0.15%胰蛋白酶-0.02鞹A 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞，经过2~3 代，即可得到纯化的乳腺上皮细胞系。

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物；培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感，据此可在传代过程中将二者分离纯化，获得纯细胞系；混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞；再加0.15%胰蛋白酶-0.02%EDTA 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞，经过2~3 代，即可得到纯化的乳腺上皮细胞系。

The findings of the newly identified goose paramyxovirus strain GPMV/HBexperimental infection were as follows: The challenge caused high morbidity and highmortality.The clinical signs included severe depression, decreased appetite or anorexia,white,yellow and green diarrhea, with neural symptoms;pathological changes showed thathaemorrhage and ulceration of intestine mucosa, necrosis of spleen and pancreas,andedema of liver and kidney, uratic deposition in kidney.Eosinophilic inclusion bodies werefound in the cytoplasm of pancreas and a large quantities of fiber protein were found inintestine. Lots of hemosidefin was found in some area of liver and kidney. A number ofT、B lymphocytes reduced sharply in spleen,lymph nodes in dead geese. Transmissionalelectron microscope showed that some cells had the morphological characteristics ofmitochondria swelling, morphological changes were condensation of nuclear chromatininto dense masses,followed by its marginating against the nuclear envelope, andespecially concentration of cytoplasm association with lots of lipid droplet.

人工感染雏鹅实验中，雏鹅临床表现为高发病率和高死亡率，食欲和饮水减少，拉白色、黄绿色稀粪，伴有神经症状；常规组织学观察，脾脏和胰腺肿大，有白色坏死斑点，肠道出血和坏死，肝脏肿大变性，肾脏肿大变性有尿酸盐沉着等；用特殊染色和组织化学方法首次对人工感染鹅副粘病毒的雏鹅的重要脏器进行研究，结果表明：胰腺中发现病毒包涵体，肠道中大量纤维素以及胶原纤维增生，肾脏、肺脏大量含铁血黄素沉着，免疫器官如脾脏和淋巴结中T、B淋巴细胞大量减少；电镜下多种细胞呈现线粒体水肿，多种实质细胞的染色质固缩，染色质边集，特别是细胞内容物如脂肪滴增多的病理变化特征。

In carcinomatous mucosa, immunoreaction of the carinomatous cell nest was negative, or only remnants of positively were seen at the site of the former membrane by collagen type Ⅳ.

结果 正常组织的基底膜呈现很强的Ⅳ型胶原抗体阳性反应，在基底膜形成较粗的环形染色带；Ⅲ型胶原抗体阳性反应在细胞外间质呈连续的细丝纤维样。

The lung was fixed in 10% formalin solution and embedded in paraffin for Haematoxyffin-eosin staining to observed the form of embolisms, Masson staining to assess collagen and fibrin/fibrinogen distribution and Toluidine blue staining was to observe mast cell distribution.

以10%甲醛固定肺组织，制成组织切片，行苏木精－伊红染色观察血栓的形态，Masson染色观察栓塞血管的胶原纤维分布，甲苯胺蓝染色观察血管栓塞处肥大细胞分布。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。