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Results In control group,three weeks after the epithelization of the wound,the thickness of the hypertrophic tissue was 3-4 times of ventro ear skin. Under microscope,the dermis was hyperplasia and got thicker,consisted with amount of fibroblast cells,collagen and vessels,the collagen was untidy,nodule or vortex,and the cartilage could be observed in some region.

结果:对照组兔耳创面上皮化后3周时,增生厚度为兔耳腹侧皮肤全层厚度的3~4倍,镜下可见真皮组织明显增生、变厚,由大量成纤维细胞、胶原组织及血管组成,胶原排列不整齐,呈结节或旋涡状分布,部分可见对应处软骨细胞增生。

Myofibril fragmentation index, contents of collagen (total collagen, heat soluble collagen, heat insoluble collagen) and muscle fiber diameter were used to evaluate the histology characteristics, and meat tenderness of Chinese mitten crab from 4 different locations was analyzed by using the method of combination index.

中文摘要:摘要:采用肌原纤维小片化指数、胶原蛋白(总胶原蛋白、热溶性胶原蛋白、热残留胶原蛋白)和肌纤维直径3种评定方法,分别测定了4地区中华绒螯蟹肌肉的组织特性,并初步探讨了采用综合指数法比较肉质的嫩度。

The microstructure of the scaffold was observed under photomicroscope and scanning electron microscopy,and the in vitro degradation time,permeability,tensile-strength,porosity,shrinka...

实验结果显示:支架中的成纤维细胞、脂肪细胞及组织纤维间质完全去除,胶原纤维得到了松散,并维持其原有的天然三维网络多孔结构;该材料透水汽性处于3000g.m-2.d-1左右,适合创面恢复;体外降解时间处于25~50h之间,并可根据需要调整工艺条件控制降解时间;拉伸强度介于10.20~11.50MPa之间,具有良好的拉伸强度;收缩温度介于70~85℃之间。上述结果表明该材料已解决了猪脱细胞真皮基质渗透性差、降解速度过慢的缺点,并且其透气性和拉伸强度高、降解性优良且可控,符合组织工程支架材料的要求。

Myocardial fibrosis is characteristic by excessive fibrous tissue accumulation, collagen contents or ratio alteration in heart tissue. The abnormal collagen accrementition is the characteristic pathological change in viral heart disease including acute and chronic myocarditis and partial dilated cardiomyopathy.

心肌纤维化是指心肌组织中胶原纤维过量积聚或胶原成分和/或比例发生变化,是病毒性心脏病(包括急、慢性心肌炎和部分扩张型心肌病)的特征性病理表现。

The results showed that ZBC can improve the rats mental state, diet, hair, increase their body weight,decrease remarkably ALT, AST, and GGT activity, raise ALB content and A/G, decrease the levelof serum HA, LN, PC III and IV-C, and decrease Hyp content of liver tissue, restrain mRNA expression of III collagen, relieve remarkably the inflammalion damage, fatly degeneration of the liver cells, and reduce the collagen fibre of the liver.

枳鳖胶囊可明显改善肝纤维化大鼠的精神状态、饮食、毛色泽,增加其体重;显著降低血清AST、ALT、GGT,显著升高血清ALB、A/G;降低血清HA、LN、PCⅢ、Ⅳ-C和肝组织Hyp含量;明显减轻大鼠肝细胞损害、肝脏脂肪变性和胶原纤维增生的程度;抑制Ⅲ型胶原mRNA的表达。

Results Histologically, the tumor was composed of mildly cellular benign-appearing spindle-shaped cells and bands of hyalinized collagen in varying proportions from area to area, and a typical patternless archite...

结果镜下见肿瘤组织由不同比例的纤维母细胞和胶原构成,纤维母细胞分化良好,形态多样,散乱穿梭于胶原蛋白束之间或环绕在玻璃样变的血管周围,伴有局灶性致密区域;胶原成分发生玻璃样变,局部呈轮辐状或瘢痕状排列。

MethodsPrimary nasal pterygial tissues(15 eyes) and normal human conjunctival tissues(8 eyes) were determined by immunohistochemical staining, using monoclonal and polyconal antibodies specifically against MMP-3, LN and col Ⅲ. The intensity of reaction on individual sections was semiquantitatively graded.

方法应用S-P免疫组织化学染色方法检测15例翼状胬肉组织及8例正常球结膜组织中MMP-3、LN、col Ⅲ的免疫学定位及表达情况,用Masson及VG染色法检测各标本中胶原纤维及弹力纤维的分布情况。

Results:(1) In clinical pathology, swelling, denatured, even necrosis and putrescence could be picked out; Collagen fibers in tendon remarkedly swelled, and tissue structure disarranged, and collagen even ruptured, broken and dissolved; Obvious denature, apoptosis and necrosis could be found in tendon cells. There appeared to be a big accumulation of inflammatory cells.

结果 (1)临床病理组织学观察发现:肌腱组织肿胀、变性、甚者坏死、腐败;肌腱内胶原纤维肿胀明显、组织结构紊乱,失去正常排列,甚者出现胶原断裂、破碎、溶解等现象;腱细胞可见明显的变性、凋亡和坏死;病变部位可见大量炎性细胞聚集。

The hFCECs were cultured by sticking tissues piece method that the cornea were divided into the limbus and central tissue piece and digestive method,respectively.For digestive method,the digestive juice of 5mg/ml DispaseⅡ+0.25%trypsin was chosed.D/F12 with 10%fetal bovine serum and 100 U/ml penicillin and streptomycin were used in this study,the culture condition was 37℃and 5%CO2,and culture medium changed every three days2、Passage of hFCECsAfter more than 80%confluenced,cells from corneal limbus hFCECs were digested for 5-15min with different concentrations of trypsin/EDTA at 37℃,and then passaged at a ratio of 1:2.The cells were subcultured on empty plates,mouse 3T3 fibroblast feeder layer,fetal corneal stromal cell feeder l ayer or HTK feeder layer respectively.And D/F12, mouse 3T3 fibroblast conditioned medium,fetal corneal stromal cells conditioned medium or HTK conditioned medium with 10%FBS and 100 U/ml penicillin and streptomycin were used respectively as the culture medium.And the culture medium were changed every three days as well.

方法一、人胎儿角膜上皮原代和传代培养1、原代培养严格无菌操作获取人胎儿角膜片,采用组织块贴壁法、酶消化法原代培养人胎儿角膜上皮细胞。2、传代培养角膜缘部的细胞生长融合达80%以上,不同稀释浓度的胰酶/EDTA消化液消化传代分别接种于空板、小鼠3T3成纤维细胞饲养层、胎儿角膜基质细胞饲养层及HTK饲养层,培养液分别采用D/F12、小鼠3T3成纤维细胞条件培养液、胎儿角膜基质细胞条件培养液、HTK条件培养液。

Results Astragaloside significantly lowered the mortality of DCM (35% vs 56%, P.05); PICP and PICP/PINP ratio were decreased in mice treated with astragaloside. Increase of CVF and Collagen I deposition in DCM was degraded in group Astr.

结果黄芪甲甙明显降低小鼠死亡率(35%vs 56%,P.05);降低血清中胶原前肽PICP含量及PICP/PINP比率;组织病理学检测结果表明黄芪甲甙显著降低心肌组织中CVF及Ⅰ型胶原纤维的堆积。

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