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纤维素酶

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Under the optimized conditions as follows: ratio of apple pomace and cottonseed meal 1︰1, urea content 2.6%, KH2PO4 content 0.09%, glucose content 2%, initial moisture content 62.9%, fermented at 30℃ for 60 h, 5662 and 30000 U/g of xylanase and cellulase were produced, respectively.

在最佳条件(基料棉粕和苹果渣比例为1︰1,第2氮源尿素2.6%,无机盐KH2PO4 0.09%,速效碳源葡萄糖2%,含水率62.9%,30℃培养60 h)下,分别获得了5662、30000 U/g的木聚糖酶和纤维素酶的高酶活发酵干曲。

The content of xylanase carboxymethyl cellulose enzyme,diastase,protein and reducing sugar are detrmined and their activity changes are discussed.

测定了木聚糖酶、羧甲基纤维素酶和淀粉酶的活性变化及蛋白质、还原糖的含量变化。并探讨了金针菇在改变了碳源的培养基上胞外酶、蛋白质、还原糖的变化。

A strain library containing 226 bacterial and fungal xylanase producers was successfully constructed, two bacterial producers Pseudomonas sp WXULI-11 and WL-11 and three fungal producers Aspergillus niger XY04B, Aspergillus niger XY07C and Aspergillus glaucus XY12D were screened out from this library.

建立了一个包括226株细菌和霉菌木聚糖酶产生菌的小型菌种库,从中筛选出了2株细菌(Pseudomonas sp WXULI-11和WL-11)及3株霉菌(Aspergillus nigerXY04B、Aspergillus niger XY07C及Aspergillus glaucus XY12D),它们的共同点是木聚糖酶生物合成量高,且不产或少产纤维素酶

8 Prepare enzyme blanks for cellulase and -glucosidase with buffer, water, and the identical amount of the enzyme.

纤维素酶和葡萄糖苷酶准备空白液,空白液包括缓冲液,水,和相似数量的酶。

Water retention value of the bleached wheat pulp decreased The fiber of refined pulp have a more amorphous area, refined pulp contains more fines, more hydrogenous fibrils, colloidal cellulosic material can be produced on the surface of fibers.

纤维素酶酶解的是一些比表面积较大,亲水性较强的胶体性纤维,这些物质的去除也使纸浆的保水值下降。同时,酶攻击了纤维的薄弱处,使纸浆的聚合度稍有下降。

In the prebleaching of kenaf bast and pine pulps, the reaction conditions for the hemicellulase pretreatment are optimized as follows: temperature 50℃, pH 5. 0 and reaction time 2hr, as well as 0.2% of enzyme charge.

实验确定了半纤维素酶预处理的优化条件:pH=5.0,温度50℃,酶处理时间2hr和酶用量0.2%。

Asparagus& Jujube Honey Wine is developed with asparagus, fresh jujube and honey as its essentials and fruit wine yeast as its fermentation bacterial species according to the following procedures: Steaming of jujube, water addition ratio 1∶3~4, temperature 80~90℃, 30min; Preparation of asparagus juice, addition quantity of the composite enzyme of pectase and cellulase is 20~30u/ml, 90min; Addition quantity of fruit wine dry yeast 0.1%~0.2% of materials input.

摘 要:以芦笋、新鲜红枣和蜂蜜为原料,果酒酵母为发酵菌种研制生产芦笋枣蜜酒。红枣蒸煮:加水比1∶3~4,温度80~90℃,30min;芦笋汁制备:果胶酶、纤维素酶的复合酶添加量20~30u/ml,时间90min;果酒干酵母添加量为投料量的0.1%~0.2%。

Virulence test demonstrated that the recombinant AHL-Lactonase has a strong anti-pathogenic activity to Erwinia cawtovora, and the addition of AHL-Lactonase to the medium of pathogen could reduced the activity of pectate lyase, prctin lyase, cellulase and polygalacturonase after 20h.

致病性检测表明,重组AHL-Lactonase对胡萝卜软腐病菌具有较强的抗病活性,在致病菌培养基加入AHL-Lactonase培养20h之后,培养基上清之中果胶酶(pectate lyase and prctin lyase,PL)、纤维素酶(cellulase,Cel)和多聚半乳糖醛酸酶(polygalacturonase,PG)的活性都存在不同程度的减小。

The optimal pretreatment conditions of the corn stalk were determined in this thesis, And the optimal conditions of saccharification by cellulose.

通过试验确定了玉米秸秆预处理最适条件,并在自主设计组装的15L微型糖化罐中进行了纤维素酶和木聚糖糖酶的酶解糖化的最适条件的研究。

In the article,five kinds of compounded enzymes were applied in tobacco leaves alcoholization. The main chemical composition of tobacco leaf was mensurated.

摘 要:本文将蛋白酶、淀粉酶、纤维素酶、果胶酶和活性添加剂一起配制而成5种酶制剂,喷在烟叶上,对烟叶进行人工发酵。

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