纤维素酶

Compound enzymes of cellulose,pectolase and papain with ratio of 2 : 1 : 1 were used to isolate polysaccharides from laminaria,and influence of two different ways of addition of compound enzymes on polysaccharide extracting rate and polysaccharide content were studied.

采用复合酶法(纤维素酶、果胶酶、木瓜蛋白酶，比例为2：1：1)从海带中提取3种多糖，并分析了不同加酶方式对海带多糖提取率和含量的影响。

BFEPP has a designed productive capacity of 30,000 standard tons each year, a production level well ahead of its domestic counterparts. Now BFEPP has 11 lines of products, including amylase765, basified protease2709, neutral protease1398, acid protease, pullulanase, cellulase, pectinase, carbohydrase,β-glucans, biological pesticide, intermediate-temperature amylase element and high-temperature amylase element.

该厂是北京市认定的高新技术企业，年设计生产能力为30000标吨，现在形成12大系列产品，其中包括：淀粉酶765、碱性蛋白酶2709、中性蛋白酶1398、酸性蛋白酶、异淀粉酶、纤维素酶、果胶酶、糖化酶、β-葡聚糖酶、高温淀粉酶、淀粉糖和。

In feed add glucanase, xylanase, cellulose-based compound enzyme enzymes, anti-nutritional factors can degrade into small molecules fragments, the release of nutrients, reduce chyme viscosity, so that nutrition the material can be fully digested and absorbed to reduce the ileum bacteria substrate, inhibiting growth and reproduction of harmful micro-organisms to prevent diarrhea.

在饲料中添加以-葡聚糖酶、木聚糖酶、纤维素酶等为主的复合酶制剂，能够将抗营养因子降解成小分子片段，释放营养物质，降低食糜粘度，使营养物质能被充分消化吸收，减少回肠中细菌的底物，抑制有害微生物生长繁殖，防止腹泻。

Recent research on the material pretreatment,factors of enzymolysis,mechanism and technology in the process of enzymic hydrolysis for cellulose were summarized in detail.

本文对纤维素酶法水解过程中有关原料预处理、影响酶解的因素、酶解机理及酶解工艺等方面的研究近况进行了较详细综述。

Otherwise, the application of chemical fertilizers enhance the activities of saccharase and phosphatase, as while, to enhance its root vigors in the appalication of organic and conventional fertilizer. The activities of urease was the highest at the treatment with 72000 kg/hm2 mature of human (296.8 mgNH3-N/g DW), the activities of proteinase was the highest at the treatment with 36000 kg/hm2 mature of duck (5.15 mg NH2-N/g DW), the activities of cellulase was the highest at the treatment with 36000 kg/hm2 pig mature (0.869 mg glucose/g DW), the activities of saccharase and phosphatase were both the highest at the treatment with 1980 kg urea, 3600 kg/hm2 Ca3(PO4) and 1305 kg/hm2 K2SO4, having the value of 13.10 mgglucose/g DW and 44.46 mg P2O5/100g DW respectively.

土壤脲酶活性以施用72000 kg/hm2人粪尿的处理最高（296.8 mgNH3-N/g DW），蛋白酶以施用鸭粪36000 kg/hm2的处理最高（5.15 mg NH2-N/g DW），纤维素酶以猪粪36000 kg/hm2的处理最高（0.869 mg葡萄糖/g DW），蔗糖酶、磷酸酶活性均以施用1320 kg/hm2尿素、2400 kg/hm2 Ca3(PO4)和870 kg/hm2K2SO4的处理最高，分别达到13.10 mg葡萄糖/g DW和44.46 mg P2O5/100g DW。

Results When cellulase and pectinase compound,amlyse and diastatic enzyme were added in order in the method of stepwise biocatalysis,98% diosgenin from plants was extracted.IR spectrum of the product was in accordance with that of reference substance and the purity of it is more than 95% according to its HPLC chromatogram.

结果利用阶梯生物催化法，当纤维素酶和果胶酶复合酶制剂、淀粉酶以及糖化酶按顺序依次加入时，能够将植物中98%的薯蓣皂苷元提取出来，其产品的红外谱图与对照品谱图相吻合，经HPLC谱测定薯蓣皂苷元质量分数95%以上。

The expressing characteristics of cellulase activity of a stable and efficient composite microbial system was analyzed by measuring CMC saccharogenic power.

用羧甲基纤维素钠糖化力法测定了一组高效稳定的纤维素分解菌复合系 MC1纤维素酶活性表达特性。

Each variety was sown into three blocks.They were harvested at 79～83d(MS1), 86～89d(MS2),93～96d(MS3) and 98～100d(MS4) after seeding and ensiled with four different ensiling treatments(R group:adding lactobacillus,3×10~9cfu/t,E group: adding cellulase 2×10~4u/kgDM,xylanase 6×10~5 thousand u/kgDM andβ-glucanase 5×10~5 u/kgDM,R+E group:adding lactobacillus 3×10~9cfu/t and enzyme preparation as E group together,group:adding no additives but carrier).The whole crop rice were pestled by mallet and then chopped into 3cm～5cm after they were harvested and then ensiled in a 6L polyethylene pot and sealed.

为探索全株青贮饲料稻的适宜品种、适宜收获时期和适宜青贮处理方法及其品质评价，2006年，选择饲料稻湘早籼24、湘丰早119和浙辐802，采用相同的栽培技术，种植在同一大田，每个品种设三个重复小区，分别在四个不同成熟阶段(MS1：播种后79～83d；MS2：播种后86～89d；MS3：播种后93～96d；MS4：播种后98～100d)收割，采用四种青贮添加剂处理(R组：添加乳酸菌3g/t；E组：添加纤维素酶2万u/kgDM、木聚糖酶60万u/kgDM和β-葡聚糖酶50万u/kgDM；R+E组—添加乳酸菌+复合酶，其中乳酸菌添加量同R组，复合酶添加量同E组；K组—不添加任何添加剂)。

The main kinds of the "SongXue Brand" are high-transformation type amylum saccharified enzyme,high-temperature resistant α-amylase,BAA- middle temperature resistant amylase ,pepsin,cellulase,feed complex enzyme and alcohol instant active dry yeast.

主要品种有&松雪&牌：高转化率糖化酶、耐高温α-淀粉酶、BAA-中温淀粉酶、酸性蛋白酶、纤维素酶、饲料复合酶和酒用活性干酵母、&天科&牌α-乙酰乳酸脱羧酶。

Vmutageniz, We gained a stain of Aspergillus niger S13 with high cellulase production, Further more we research on the optimum solid culture a wheat stem 70 % bran 30 %, nitrogen source is yeast extract 0.68%,(NH4)2SO4 0.35%, trace element 0.1%, Vogel's solution 4 %, pH3.5, temperature 30℃, cultivation time 72-96hr, With such condition, its β-glucosidas activity reached 801.18mg·glucose/g·h, CMCase activity 6262.46mg·glucose/g·h filter paper activity 20.73IU.

本文通过分离筛选和紫外诱变法，选充出一株高产纤维素酶黑曲霉菌株S13，并进行了最佳固态发酵培养条件的研究，其结果为：最佳碳源为1％碱洗麦杆粉70％，麸皮30％；另加酵母膏0.68％，(NH_4)_2SO_4 0 35％，微量元素0.1％，Vogel's母液4％，初始pH3.5培养温度30℃，培养时间为72～96 小时，在此条件下其β-葡萄糖苷酶活力达801.18mgG/h·g，CMC酶活6262.32mgG/h·g，滤纸酶活20.73IU。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。