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纤维素酶

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To investigate the effects of lipoxygenase (LOX-1) on the cormels formation and enlargement in Gladiolus hybridus, with 'Rose Supreme' and 'Advanced Red' as materials, LOX-1 activity, endogenous methyl jasmonate, sucrose, starch and cellulose contents were examined during the cormels growth.

为探讨脂氧合酶(LOX-1)对唐菖蒲籽球形成和膨大的影响,以'Rose Supreme'和'Advanced Red'两个品种为试材,研究了籽球的生长发育与LOX-1活性,内源茉梨酸甲酯、蔗糖、淀粉和纤维素含量变化之间的关系。

The long specific DNA probes were synthesized by polymerase chain reaction in the highly specific gene sequences of above 10 bacteria. The species-specific probes synthesized were inoculated on a nitrocellulose filter and hybridized with template DNA labeled with long-arm photosensitive biotin. The signal of hybridization reaction was observed to detect which bacteria infected. The sensitivity and specificity of the method were studied.

在这10种病原菌高度特异的基因序列内利用聚合酶链反应合成上述10种细菌特异的长链DNA探针,并将合成的特异探针固定在硝酸纤维素膜上,与用长臂光敏生物素标记后的模板DNA进行斑点杂交反应,通过观察杂交信号判断存在何种细菌感染,同时研究该方法的敏感性和特异性。2。

In order to preserve the color of fresh-cut peach fruits and extend its shelf life, effects of three kinds of edible coatings(carrageenan, carboxymethyl cellulose, sodium alginate)and their combinations in two of them on qualities and oxidase activities of fresh-cut peache fruits during storage at 5℃ were investigated.

为了保持鲜切桃块的色泽,延长其货架期,试验以久保和绿化9号桃为材料,研究了卡拉胶、羧甲基纤维素、海藻酸钠涂膜及其两两复配处理对鲜切桃在5℃下贮藏期间的色泽及多酚氧化酶活性变化的影响。

NSC-7 could degrade 73.6% of rice straw within 14 d. 10 kinds of material were detected in volatile products by gas chromatography mass spectrometry, and they were mainly acetic acid, glycerol, butyric acid, and propanoic acid. NSC-7 could retain stable degrading ability and high cellulase activity after preserved in -80℃ and freeze-drying for four years. It could remain its degrading ability after 90℃ of high temperature treating for 30 min, and resume its degrading ability after subcultured 2 times after 105℃ of high temperature treating for 30 min in autoclave.

NSC-7在14 d的培养过程中,使稻秆分解73.6%;用GC-MS测定结果发酵液中检测到10种化合物成分,其中峰值较大的依次为乙酸、甘油、丁酸、丙酸; NSC-7在-80℃冷冻和冻干条件下保存4年后仍具有稳定的秸秆分解能力和纤维素内切酶活性;经90℃高温处理30 min后,仍能够保持分解能力, 105℃处理30 min后转接2次就能恢复分解能力,显示出很高的保存稳定性和热稳定性。

NSC-7 could degrade 73.6% of rice straw within 14 d. 10 kinds of material were detected in volatile products by gas chromatog- raphy mass spectrometry, and they were mainly acetic acid, glycerol, butyric acid, and propanoic acid.

NSC-7在14d的培养过程中,使稻秆分解73.6%;用GC-MS测定结果发酵液中检测到10种化合物成分,其中峰值较大的依次为乙酸、甘油、丁酸、丙酸;NSC-7在-80℃冷冻和冻干条件下保存4年后仍具有稳定的秸秆分解能力和纤维素内切酶活性;经90℃高温处理30min后,仍能够保持分解能力,105℃处理30min后转接2次就能恢复分解能力,显示出很高的保存稳定性和热稳定性。

NSC-7 could degrade 73.6%of rice straw within 14d. 10 material were detected in volatile products by gas chromatography mass spectrometry,and it was mainly acetic acid, glycerol, butyric acid, and propanoic acid. NSC-7 could retain stable degrading ability and high cellulase activity after preserved in -80 and freeze℃-drying for four years. It could remain its degrading ability after 90 of high ℃temperature treating for 30min, and resume its degrading ability after subcultured 2 times after 105 ℃of high temperature treating for 30min in autoclave.

NSC-7在14d的培养过程中,使稻秆分解73.6%;用GC-MS测定结果发酵液中检测到10种化合物成分,其中峰值较大的依次为乙酸、甘油、丁酸、丙酸;NSC-7在-80℃冷冻和冻干条件下保存4a后仍具有稳定的秸秆分解能力和纤维素内切酶活性;经90℃高温处理30min后,仍能够保持分解能力,105℃处理30min后转接2次就能恢复分解能力,显示出很高的保存稳定性和热稳定性。

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