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纤维素酶

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In this study, the whole components of the cellulase system in SM-L22 was separated and analyzed.

本项研究对曲霉SM-L22的纤维素降解酶系进行了全组分的分离与分析。

We found that both PNIP and PNIP—enzyme labelled antibody could adhere quickly and tightly to the cellulose acetate and nitrate membrane.

在研究中我们发现,PNIP和PNIP与酶标抗体的复合物可以快速紧密地吸附到醋酸、硝酸纤维素膜上。

D. During the course of our study, we found that both PNIP and PNIP-enzyme labelled antibody could adhere quickly and tightly to the cellulose acetate and nitrate membrane either below or above the LCST.

在高于LCST的温度下过滤,其保留率较高;而抗原、抗体及酶标抗体等蛋白质对膜的吸附作用很弱,可以通过洗涤滤过纤维素膜。

When cellulose nitrate membrane was used as the carrier of HBsAg, examination with immunoenzyme spot method could give directly visible result.

以硝酸纤维素膜作HBsAg载体,用免疫酶斑点法检测,结果直观,方法简便、易行,因HBsAg吸附牢固,可用冲洗法去除残留消毒剂。

Jiangxi Jinwei Biological Product Co.,Ltd is a hi-tech shareholding company specialized in researching and processing of animal and plants and biochemical medicine .mainly products including Catalase,Polishing Ferment and Industrial Pancreatin etc used in texitle industry .

江西金伟生物制品有限公司是专业致力于动植物,生化药物研究及加工的股份制高新技术企业。公司主要产品:用于纺织服装制作的过氧化氢酶,抛光型纤维素霉和工业胰酶。

By the liquid-state fermentation, the best factors for cellulase from C. thermophile were investigated. The factors including carbon sources, nitrogen sources, the original pH of medium and varieties of pH and the protein concentration during the cultural process were tested .

采用液体发酵培养法,通过对碳源、氮源、培养时间、液体培养基的起始pH值及产酶过程中培养液中pH值和蛋白质含量变化的研究发现:在以2%纤维素、1%可溶性淀粉为碳源,2.0%KNO_3+0.2%酵母为氮源,液体培养基的起始pH值为6.5,50℃下液体发酵培养8d~9d后,各种酶活力最高。

This method is more simple and inexpensive than screening cDNA library, and can be easily adapted to clone other genes. An Amorpha fruticosa cDNA clone encoding UDP-glucose pyrophosphorylase, a key enzyme producing UDP-glucose in the synthesis of sucrose and cellulose, was cloned by using this method.

本论文利用此方法首次从紫穗槐中克隆了与纤维素合成前体----尿苷二磷酸葡萄糖的代谢有关的尿苷二磷酸葡萄糖焦磷酸化酶基因和与木质素合成相关的4-香豆酸:CoA连接酶(4CL)基因的全长cDNA序列,并由此推测出相应的氨基酸序列。

Acid phosphatase from Branchiostoma beleheri was isolated and purified by ammonium sulfate fractionation,Sephadex G-75 gel filtration and CM-cellulose chromatography under acid condition.

从厦门文昌鱼Branchiostoma belcheri分离提纯酸性磷酯酶(EC3.1,3.2),进一步经Sephadex G-75和羧甲基纤维素(CM-52)柱层析纯化,获得聚丙烯酰胺凝胶电泳单一纯酶制剂,比活力为351μm/min mg。

The direct bioconversion process of cellulosic materials into ethanol involves the enzyme synthesis, cellulose and semicellulose hydrolysis, and ethanol fermentation using glucose and xylose.

植物纤维资源直接发酵生产乙醇涉及到酶的合成,纤维素的酶水解和葡萄糖、木糖的乙醇发酵等步骤,它们对最终发酵结果有着不同程度的影响。

The production and characterization of endoglucanase from Streptomyces xylophagus KX6 was studied. Maximum endoglucanase yield of 0.538 IU/ml was achieved with medium pH8.0, containing CMC2Na 1.0% as carbon resource, soybean meal 1% as nitrogen resource, 2% inoculating volume, 30% 250 ml triangle flask bulk for medium volume at 40℃ 200r/min shaker for 48h.

实验中对其产酶的液态发酵条件进行了研究,碳源为1%羧甲基纤维素钠,氮源为1%豆粕粉,250ml三角瓶30 %装液量,接种量为2%,培养基初始pH为8.0,培养温度为40℃,200r/min培养48h后,发酵液中内切葡聚糖酶活达到0.538IU/ml。

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