纤维素酶

Also, it could be used to study the effect of cellobiose on cellulase activities and the inhibition of dyestuff in the enzymatic treatment bath against cellulase activities.

滤纸崩溃法可以测定某些滤纸糖化法无法测定的中性酶和弱碱性酶的总活力，也可用来研究纤维二糖对纤维素酶活力的影响，以及染料溶液对纤维素酶活力的抑制等。

Because of specificity and high efficiency, the catalytic efficiency of cellulase is high(more 103~1013 times than general catalysts), the condition of catalytic reaction is mild and the catalytic activation can be adjusted and controlled, which can improve remarkably semblance and handle of fabrics during finishing fabrics by biological catalyzing, moreover cellulase can replace some chemical materials such as acid, cocain, chlorine and phosphorus etc, so as to decrease pollution, make no waste-water and protect environment.

由于酶具有专一性和高效性，采用生物催化，其催化效率高比一般催化剂高10~3～10~(13倍，催化反应条件温和，催化活性可调节控制，应用在纺织品处理上，能显著改善织物的外观和手感，并能取代一部分酸、碱、氯、磷化合物等化工原料，从而减少污染，不产生污水，有利于环保和生态。而且酶本身又无毒，容易生物降解，不会引起环境污染，所以酶处理已被公认为是一种符合环保要求的绿色印染加工方法。本文选用了市场上常用的纤维素酶R-10和纤维素酶ECS2610两种生物酶。

This paper studied the extraction technology of pectase and cellulase method of raffinose in cottonseed cake.

本文对棉籽饼粕中棉籽糖的果胶酶法和纤维素酶法提取工艺进行了研究，实验结果表明：果胶酶和纤维素酶均可较显著地提高棉籽糖的提取率，分别是乙醇法提取的117%和139%。

In this research, the activities of CWDE were measured by saccharifying according to the theory that red-brown amino compounds were produced when 3, 5-dinitro salicylic acid and aldehyde were heated together. And the testing conditions were made clear. The activity of Cx should be tested with optimal temperature of 50℃, optimal pH of 4.8, incubation time of 30 min, and optimal substrate of 0.6% CMC-Na. Due to the heat-libality, Cx should be sterilized more than 10 min. The testing conditions of the activity ofβ-G were almost the same with Cx except the substrate of 0.5% salicin and the sterilization time of more than 15 min.

主要取得了一下成果：(1)明确了条锈菌细胞壁降解酶活性测定的条件：羧甲基纤维素酶活性的测定条件为50℃，pH4.8，反应时间30min，底物浓度为0.6%羧甲基纤维素钠，以DNS法测定，且失活酶液的灭活时间应大于10min；β-葡萄糖苷酶活性的测定条件为：50℃，pH4.8，反应时间30min，底物浓度为0.5%水杨苷，以DNS法测定，且失活酶液的灭活时间应大于15min；果胶酶活性的测定条件为：50℃，pH4.8，反应时间1h，底物浓度为0.25%果胶，以DNS法测定，且失活酶液的灭活时间应大于10min。

Study on the optimal conditions of cellulase from termites The optimal pH when Cx from Reticulitermes flaviceps reacted with substrate sodium carboxymethylcellulose was between 5.0-7.4 and 5.0-6.2 when BG enzyme was reacted with substrate salicin, the relation between activities of enzymes and relevant pH values accorded with Quadratic models.

一、白蚁纤维素酶最适反应条件研究黄胸散白蚁 Reticulitermes flaviceps C_N酶与羧甲基纤维素钠(Carboxymethylcellulose sodium，CMC-Na)反应的最适pH值范围在5.0-7.4之间，BG酶与Salicin反应的最适pH值为5.0-6.2，2种酶的活性均与pH值符合二次项曲线模型。

The formation of silique dehsicence susceptibility was investigated in terms of water content, synthetic rate of ethylene, enzyme activity of exo polygalacturonase and end cellulase , cell alteration in dehiscence zone in silique and silique dehiscence susceptibility during silique development.

从油菜角果发育过程中角果含水量、乙烯合成速率、外切 PG 酶和内切纤维素酶活性，以及角果裂区切片观察等方面研究了角果开裂敏感性形成的原因⒚花后50d 的角果果柄端壳状果瓣和线状果瓣间的裂区薄壁细胞膨大，在高倍光学显微镜下可见胞间空隙开始形成⒚此前6d 内乙烯合成速率迅速上升并出现第二次合成高峰，与之同步发生外切 PG酶和内切纤维素酶活性急剧上升，而乙烯合成第二高峰出现于角果含水量低于80％以下⒚由此看来，角果失水引起乙烯合成第二高峰出现，从而导致水解酶活性上升，最终导致果瓣裂区薄壁细胞膨大，分离和胞间空隙形成及角果开裂敏感性产生

Aspergillus WQ is selected to explore the factors which affect producing enzymes. The best carbon source is crystallitic cellulose+straw powder, the best nitrogen source is peptone+yeast abstract cream, the best growing temperature is 35℃and the best initial pH is 5.5. WQ can produce the largest quantity of enzymes after being cultivated eight days.

从50℃左右牛粪自然堆肥中筛选了一株产酶量较高的曲霉WQ，对影响其产纤维素酶的因素和酶的热稳定性进行了初步研究，结果表明：液体发酵时，该菌最佳碳源是微晶纤维素+稻草粉组成的复合碳源，最佳氮源是蛋白胨+酵母膏的复合氮源，最适生长温度为35℃，最适起始pH值为5.5，在生长第8天产酶量最高。

Industrial pectic enzyme preparations may also contain cellulase, hemicallulase, xylanase, and protease.

工业的果胶酶制剂可能含有纤维素酶、半纤维素酶、木聚糖酶和蛋白酶。

The activity of polygalacturonase,PME,cellulase and the soluble pectin content of MA stor-age were significantly lower than those of control; while the flesh firmness,cellulose content ,protopectin content were sig-nificantly higher than those of control. Results showed that MA storage retarded the softening process of mulberry fruit.

研究了在(2±1)℃下，MA贮藏对桑果细胞壁组分和水解酶活性的影响，结果表明：MA贮藏可抑制桑果呼吸强度和乙烯释放量，MA贮藏桑果的纤维素酶、果胶甲酯酶和多聚半乳糖醛酸酶等细胞壁水解酶活性和水溶性果胶含量显著低于对照，而果实的纤维素和原果胶含量显著高于对照，从而延缓桑果的软化。

Taking the wild snail Su bag as the raw material to extract the snail enzyme product,and determining the activity of cellulose enzyme and starch enzyme .

以野生蜗牛嗉囊为原料提取蜗牛酶产品，检测所含纤维素酶、淀粉酶的活性，对其蜗牛酶产品进一步测试，结果表明新疆蜗牛酶中的纤维素酶活力为450U/g 淀粉酶为405U/g。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。