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纤维素酶

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It was shown,based on the study,that cellobiose utility capability of the recombinants was increased,glycerol productivity of that was decreased,and the bglⅡ gene expression was stable in t.

结果表明,整合子利用纤维二糖的能力得到提高,产甘油能力下降,外源基因表达稳定;引入外源基因后,其对酵母增殖能力没有大的影响,仅形态发生变化;在以微晶纤维素为原料,结合纤维素酶发酵时,与亲代工业酿酒酵母相比,发酵液乙醇浓度最高增加69%。

The mathematical model of cellulase adsorption onto cellulose was constructed.

构建了纤维素酶对纤维素吸附和水解的数学模型。

The paper summarized the different kinds of cellulolytic fungi, then give a brief introduction of cellulase producing methods.

总结了常见的分解纤维素真菌种类,并介绍了纤维素酶生产的方法。

The fungus were firstly screened in Congo red plate for cellulase, and then cultured in residues of chinaberry as only carbohydrate supply.

采用纤维素刚果红平板培养基,对采自陕西秦岭山区的土样和腐烂枝干进行初筛,再以川楝残渣为惟一碳源进行摇瓶培养复筛,最后对获得的高纤维素酶活力菌株进行形态学和分子生物学鉴定。

Most cellulases from T. reesei consist of a catalytic domain and a smaller non-catalytic cellulose-binding domain connected by a linker region.

大多数瑞氏木霉纤维素酶由纤维素结合结构域和催化结构域组成,它们之间通过链接区连接。

The bioinformatics analysis showed that the gene had the highest homology with the gene of Penicillium janthinellum, which reached up to 76%. The first 26 amino acids might be a signal peptide sequence with the hydrophobicity of up to 2.63. The CBHⅠ gene consisted of the catalytic domain, convergence zone and cellulose-binding domain and its tertiary structure was mainly composed of 3-sheets.

分别扩增到鹅源草酸青霉F67纤维素酶CBHⅠ基因片段A(EU574736)、5'端序列B1(EU603295)、3'端序列B2(EU652768)及全长基因C(EU727171),并成功构建真核表达载体pPIC9K-CBHⅠ;生物信息学分析表明,该基因蛋白序列与微紫青霉氨基酸序列同源性最高,达76%,前26个氨基酸为信号肽序列,疏水性可达2.63,由催化功能域、衔接区和真菌性纤维素结合域构成,其三级结构主要为β-sheet。

The fermentation product of Acetobacter xylinuml.1812 was hydrolyzed by acid or cellulase, then it was proved that there was glucose in the hydrolyte by TLC and chromatography mass spectrometry, thereby the product was identified as bacterial cellulose.

通过薄层层析和气-质联谱测得木醋杆菌发酵产物经酸或纤维素酶水解后的水解液中主要成份为葡萄糖,从而鉴定出发酵产物为细菌纤维素。

In view of the high efficiency and the environment protection, the degradation catalysed with cellulase is the most effective way to hydrolyze cellulose thoroughly and causes no pollution.

从高效和环保的角度出发,纤维素被彻底分解而无污染的一条有效的途径便是利用纤维素酶的水解作用。

Several types of surfactants were adopted to organic solvent mediae in order to investigate the characteristics of cellulose hydrolysis use the carboxymethyl cellulose as substrate.

通过在有机相体系中添加几种不同属性类型的表面活性剂,在此体系中以羧甲基纤维素为底物,研究纤维素酶降解特性。

Studied the pretreatment of straw stalk, domestication of endurance of microzyme to alcohol, the influence of straw stalk material particle size, thalli intergrowth and cellulose on deoxidized sugar level and yield of alcohol.

初步考察了秸秆纤维素类原料预处理、酵母菌忍耐酒精度能力的驯化、原料粉碎度和菌体共生及纤维素酶对发酵底物还原糖含量及酒精产率的影响。

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