纤维素酶

However, CDH has little synergism with cellobiohydrolase or endoglucanase in cellulose degradation. But, if the substrate was pretreated with CDH in the presence of cellobiose, the rate of the hydrolysis of the substrate by cellobiohydrolase or endoglucanase increased.

CDH与纯化的外切纤维素酶或内切纤维素酶或内切纤维素酶之间没有协同作用关系，但在纤维二糖存在条件下底物事先用CDH处理后再用上述纯化的酶水解，作用明显增强。

The existing problems in cellulase production and application and the development trend in the future are analyzed and discussed.

简要介绍纤维素酶的酶学性质、降解机制、生产工程菌的选育、纤维素酶的应用情况，以及对纤维素酶生产与应用方面存在的问题和未来发展趋势进行了分析与探讨。

The best enzymolysis pH of cellulose was 6 and the enzymolysis temperature was 30℃.

该纤维素酶的最适酶解pH值为6，温度为30℃；Na对纤维素酶具有激活作用，Cu(上标 2+)则明显抑制纤维素酶的活性；VB2可以提高纤维素的分解率，VC使酶活力和纤维素的分解率明显升高。

The enzymatic hydrolyzing technologies of fucoidan from Laminaria japonica using cellulase and pectase were studied.

岩藻聚糖硫酸酯是一种具有多种生物活性的多糖，对用纤维素酶、果胶酶酶解制备海带岩藻聚糖硫酸酯的工艺进行了研究，正交试验结果表明：纤维素酶、果胶酶优化的酶解工艺参数为：纤维素酶0.221%、果胶酶0.074%，温度50℃，pH值4.5，时间50min。

The best combination of isolating protoplast of Mozzie buster was the enzyme mixture containing 2.0% Cellulase R-10, 0.4% Pectinase, 0.5M mannitol as osmoticpressure, 27℃under dark 80r/m rotating enzymolysis 8h, and the highest yieldachieved to 1.67×10~6per gram, the protoplast vitality was 87.4%; The bestcombination of isolating protoplast of Ocimum basilicum was the enzyme mixturecontaining 1.6% Cellulase R-10, 0.4.% Pectinase, 0.5M mannitol as osmotic pressure,27℃under dark 80r/m rotating enzymolysis 8h, and the highest yield achieved to1.67×10~6 per gram, the protoplast vitality was 87.4%.

本研究对酶解最佳条件进行研究，得出分离驱蚊草原生质体酶解条件以CPW-13进行质壁分离1h，2.0％纤维素酶和0.4％果胶酶，0.5M甘露醇为渗透压，27℃黑暗下80r／m酶解处理8h为好，产量可达1.67×10~6个／g，原生质体活力为87.4％；分离罗勒原生质体酶解条件以CPW-13进行质壁分离1h，1.6％纤维素酶和0.4％果胶酶，0.5M甘露醇为渗透压为好，27℃黑暗下80r／m酶解处理8h，产量可达2.14×10~6个／g，原生质体活力为67.8％。

High-temperature cellulolytic microbes were isolated from cow dung unartificial compost and were determined the enzyme activity of C1, CMCaae and Fpaae.

方法］从牛粪自然堆肥中分离出高温纤维素分解纤维素菌，并测定其外切纤维素酶活（C1酶）、羧甲基纤维素酶活、FPA酶活。

Ammonium oxalate among the used nitrogen-containing compounds had the most remarkable effect on the production of ligninolytic enzymes but no stimulating effect on the production of xylanase and cellulases by P.

conchatus产木素降解酶的促进作用最显著，而对半纤维素酶，纤维素酶的产生促进作用不明显，在整个培养过程中，微晶纤维素酶活力都未检测到，CMC酶活力也较低。

Ammonium oxalate among the used nitrogen-containing compounds had the most remarkable effect on the production of ligninolytic enzymes but no stimulating effect on the production of xylanase and cellulases by P.

研究发现在几种含氮化合物中，草酸胺对P 。conchatus产木素降解酶的促进作用最显著，而对半纤维素酶，纤维素酶的产生促进作用不明显，在整个培养过程中，微晶纤维素酶活力都未检测到，CMC酶活力也较低。

The protease activity in foregut was higher than that of midgut in an acidic condition at pH 5.0-5.8, while in an alkalescence condition at pH 7.0-8.6, the protease activity was higher in midgut than that in foregut. The lipase activity of foregut was high and stable in two pH ranges: 4.2-5.0 and 6.2-7.0, and that of midgut was high at pH 3.8, while the lipase lost enzyme activity at pH over 9.0. The amylase activity was high and stable at pH 6.6-7.4 in both foregut and midgut. The cellulase activity of foregut and midgut was high and stable at pH 6.2-7.4 and 5.4-7.0 respectively.

刺参前肠蛋白酶活力在酸性环境下较中肠高，且在反应pH为5.0-5.8之间酶活力相对稳定，而中肠蛋白酶活力在碱性环境下较前肠高，且在反应pH为7.0-8.6之间酶活力相对稳定；刺参前肠脂肪酶活力随着pH的升高出现两个相对稳定的峰值，分别为4.2-5.0和6.2-7.0区间，中肠脂肪酶活力在pH为3.8时达到最大值，而在pH超过9.0明显失活；刺参前肠和中肠淀粉酶活力在pH变化时表现出相似的变化趋势，在反应pH为6.6-7.4之间酶活力较高且相对稳定；刺参前肠和中肠中纤维素酶活力在pH变化时反应不一致，前肠淀粉酶在反应pH为6.2-7.4之间酶活力较高且相对稳定，中肠纤维素酶活力在反应pH为5.4-7.0之间酶活力较高且相对稳定。

Our previous studies show that sorangium cellulosum can be cultivated on simple inorganic salt medium with sterilized filter paper as the only carbon sources and can finally degrade the filter paper significantly.

我们实验室前期研究表明纤维堆囊菌能够在仅含有滤纸的简单无机盐平板上生长并有效地降解滤纸，对其纤维素降解酶酶活性分析得知，纤维素降解酶位于细胞表面并组成一个大的复合体结构，该复合体至少具有木聚糖酶和纤维素酶活力。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。