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纤维原细胞

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Result: The divided functional recipes of DHZCP could significantly decreased the deposit of lipid and the atherosclerotic plaque area in aorta intima, relieve the histopathological changes of atherosclerosis,and inhibited the proliferation of vascular smooth muscle cells and collagen to reduce pachynsis of vascular intima. The divided functional recipes of DHZCP also reduced IT, IMT and IT/MT and reversed the contractive vascular remodeling.

结果:大黄虫丸各功效组分均能减少家兔主动脉内膜脂质沉积,使AS斑块面积减小( P 。05);抑制血管平滑肌细胞增殖和胶原纤维增生,减轻内膜增厚;降低IT,IMT,IT/IMT比值( P 。05),干预收缩性血管重塑;减轻线粒体肿胀,减少泡沫样细胞形成。

Low reaction of fetus to inflammation, different extracellular matrix, component of collagen fiber and cell factors may take part in scarless healing process. Regulation of gene expression may play an important role in the process. But the concrete mechanism of scarless healing is not clear yet and still needs more researches and elucidations.

胎儿对炎症的低反应性、胎儿不同的细胞外基质、胶原纤维的成分、细胞因子可能也参与了无瘢痕愈合过程、基因表达调控在其过程中可能也起重要作用,但无瘢痕愈合的具体机制还不明了,有待进一步研究阐明。

Methods: models of traumatic knee injury were made with zelanian rabbits and were divided into three groups by different time for postoperative function exercise . group a, fixed just after operation; group b, fixed 24 h after operation; group c, fixed 72 h after operation. range of knee joint motion was measured after 30 days, the epithelial cell of synovial membrane and its proliferation, collagen fiber proliferation were observed.

采用新西兰兔制成膝关节创伤模型,然后按功能锻炼时机不同分为术后不固定组;术后固定24 h组;术后固定72 h组3组,30 d后测量膝关节活动度,观察滑膜上皮细胞以及细胞增生、胶原纤维增生情况。

Methods The disorder of myofibrils,swelling of mitochondria with broken cristae and decrease of cytochrome C oxidase activity caused by ADR were prevented by the administration of teapol.

结果 阿霉素所造成的大鼠心肌细胞肌原纤维排列紊乱、线粒体肿胀,嵴断裂及细胞色素C氧化酶活性降低,可明显地被抗氧化剂天力体保所防止。

Primordial germ cells derived from gonadal ridges and mesenteries of 30-70-day post menopause human ernbiyos, when cultured on mouse MEF or STO fibroblast feeder layers in presence of hrLW , hrbFGF, hrSCF, and forskolin could survive and give rise to multicellular colonies, resembLing mouse ES-like colonies .

从停经30-70天流产的早期胚胎后肠系膜中或生殖嵴部位分离到的原始生殖细胞,在小鼠的原代MEF或STO成纤维细胞等饲养层细胞上培养,能在hrLIF、hrFGF、hrSCF、forskolin等的共同作用下存活并增殖。

MSCs from human BM were isolated by Ficoll density centrifugation cultured and expanded in DMEMLG containing FBS. MSCs are adherent and fibroblastic, and maintained similar morphology as passaging.

从人骨髓中分离和培养间充质干细胞,在原代和传代培养中其形态学上均为贴壁、纤维状的细胞,随著代次的增加细胞形态更加趋於一致。

Results The physically and chemically treated SIS was pure with more hollows on the surface and undamaged collagen fibers. The cartilage cells could grow, adhere to and differentiate on the layer of SIS and enter into the hollows, secrete a great deal of extracellular matrices.

结果 经物理和化学处理的SIS纯度高、孔隙多,胶原纤维未受损;软骨细胞在SIS材料上生长、黏附、增殖,并能长入材料的孔隙内,分泌大量的细胞外基质成分。

To master fluid mosaic model of cell membrane;peripheral proteins and integral proteins;the type of extracellular matrix;Collagen;glycosaminoglycan and proteoglycan;elastin; Occliding junctions;anchoring junctions;desmosome hemidesmosome、the structure and function of adhesion belt and focal adhesion;cell coat;cadherins;selectin;Ig-superfamily; integrins

掌握生物膜的流动镶嵌模型;膜周边蛋白和膜内在蛋白;细胞外基质的类型;胶原纤维;糖胺聚糖和蛋白聚糖;弹性蛋白;封闭连接;锚定连接;桥粒与半桥粒、粘着带与粘着斑的结构与功能;细胞外被;钙粘素;选择素;免疫球蛋白超家族;整联蛋白

Methods Hepatic cells were obtained from fetal rats by means of mechanical separation and hanging-drop culture, and after two days of primary culture, the medium was changed for further cell culture in the presence of 20% fetal bovine serum (containing glucose 25ml/L, mycillin 100 U/ml, pH 7.6), 10 mmol/L nicotinamide, 1 mg/L insulin, affix N2, basic fibroblast growth factor, stem cell factor, epidermal growth factor and leukemia inhibitory factor.

采用机械分离法结合悬滴培养法获得胎肝干细胞,原代培养2d后改变培养基,添加含20%胎牛血清的DMEM(含25ml/L葡萄糖,100U/ml青链霉素,pH值7.6),添加烟酰胺10mmol/L,胰岛素1mg/L,N2添加剂,碱性成纤维细胞生长因子、表皮生长因子、干细胞因子、白血病抑制因子各20μg/L诱导分化为nestin阳性细胞。

In the rat carotid injury model, SM16 dosed once daily orally at 15 or 30 mg/kg SM16 for 14 days caused significant inhibition of neointimal thickening and lumenal narrowing. SM16 also prevented induction of adventitial smooth muscle alpha-actin-positive myofibroblasts and the production of intimal collagen, but did not decrease the percentage of proliferative cells.

在大鼠颈动脉损伤模型中,每日口服15或30mg/kgSM16持续14天能够显著抑制新生内膜增厚及管腔狭窄,预防对外膜α-SMA阳性肌成纤维细胞的诱导和内膜胶原的合成,但并不降低增殖细胞的比例。

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