纤维原细胞

METHODS: Detection of the cell proliferation by MTT method and ALP expression by enzyme dynamics method.

原代培养牙髓成纤维细胞、牙周膜成纤维细胞，用MTT法测定细胞增殖，酶动力学方法测定ALP活性。

Comparisons between the cell lines were made on the basis of time to observed cytopathic effects, ultra structure changes, titer in 50% tissue culture infectious doses, and the alterant distribution of DNA content in cell cycle. The results showed that, under identical conditions, various degrees of CPE could be found in the Hep-3B, A549 and NIH 3T3 36h post infection of BTV-10, while there was no CPE could be observed in HEL.

随后在对BTV-10体外感染特性的研究中，我们在相同条件下，将该病毒接种于1株原代人胚肺成纤维细胞(Human embryonic lung fibroblast cells，HEL)和3株传代细胞株：人肝癌细胞(Human hepatic carcinoma 3B cells，Hep-3B)；人肺腺癌细胞(Human lung carcinoma A549 cells，A549)；鼠正常成纤维细胞(Normal mouse fibroblastscells，NIH 3T3)。

Results Osteoclast and osteoblast around trabecula of bones showed proliferation, shi1e fatty tissues in bone marrow decreased and hematopoietic tissue well-regenerated alter the hyperbaric oxygen-treated. Also juvenal osteocyte and collagen fibril were observed in early stage by electron microscope.

结果 多数骨小梁周围可见骨母细胞增生、偶见破骨细胞增生，髓腔内脂肪细胞逐渐减少，造血组织增生；电镜发现骨小梁内早期即出现幼稚骨细胞和胶原原纤维合成。

RESULTS: BMP-2 was mainly found in the plasma of fibroblast, osteoblast, cementocyte, odontoblast separately.

结果：牙周膜成纤维细胞、成骨细胞、成牙骨质细胞及胶原纤维中均可见BMP-2阳性染色。

Results In model group, alveolitis increases (much inflammatory cell infiltration), pulmonary interstitial fibroblast increases.

结果］模型组7d时肺泡腔内大量炎性细胞浸润，可见少量成纤维细胞，Ⅰ型细胞受损，Ⅱ型细胞明显增生；28d时肺泡炎减轻，肺间质和胸膜层胶原纤维明显增多。

E studied expression levels of WAVE1 in six leukemia cell lines (the human Jurkat T leukemia cells, U937 histiocytic lymphoma cells, Burkitts lymphoma cell Raji, acute promyelocytic leukemia cell HL-60, chronic myelogenous leukemia cell K562 and K562/A02) by Western blotting analysis. Levels of WAVE1 expression were high in all six human leukemia cancer cell lines. In contrast, the constitutive expression levels of WAVE1 were noticeably lower in normal human peripheral blood mononuclear cells, or non-blood cancer cell-lines, including human lung A549 cancer cells, Hela cervical cancer cells, MG-63 osteosarcoma cells, CNE2 nasopharyngeal carcinoma cells, human umbilical vein endothelial cell, QSG7701 hepatocarcinoma cells, and WI-38 lung fibroblastoma cells.

AVE1在人类血液肿瘤细胞系(人T细胞白血病细胞系Jurkat、人组织细胞淋巴瘤细胞系U937、人Burkitts淋巴瘤细胞系Raji、人原髓细胞白血病细胞系HL-60、人慢性髓原白血病细胞系K562和K562／A02)中高表达，在非血液肿瘤细胞系(人肺腺癌细胞系A549、人宫颈癌细胞系Hela、人成骨肉瘤细胞系MG-63、人鼻咽癌细胞系CNE2、人脐静脉内皮细胞系HUVEC、人肺成纤维细胞系WI-38、人肝上皮细胞系QSG7701)以及正常人外周血单个核细胞中低表达或不表达。

The ligamentous scaffold material were made from the PVA fibers by braiding technique. The mechanical properties of the scaffold materials were measured using the electronic materials testing machine. Human ACL cells were isolated and cultured by tissue explant and collagenase digestion methods, the morphous characteristics of cells and the secretion of collagen protein were measured. In order to observe the biocompatibility between the material and cells, ACL cells were in vitro expanded and seeded on the scaffold materials.

方法］用聚乙烯醇纺丝纤维编织构建韧带支架材料，在电子拉力机上测试该支架材料的力学性质；用组织块和胶原酶消化培养法在体外分离培养人前交叉韧带细胞并对细胞的生长形态和分泌胶原蛋白的特征进行检测，细胞经体外扩增后种植于编织构建的聚乙烯醇韧带支架材料上观察。

P＜0.01. 28d, q=4. 0452, p＜0.05 . Corresponding pathologic result shows that the corneal stromal of height edema increase thickness to have infiltration of inflammation cells with hyperplasia of fibroblast cell and large vessel. The collagen fiber in stromatic layer is dissolved and destroyed seriously, suggesting that in addition to HSV-1's direct destructuring effect, inflammatory cells that might play an important role in the pathology of HSK induce the immune-pathological reaction.

相应的组织病理学研究结果显示（UV-B 14天）：病毒对照组角膜基质层高度水肿、增厚，并有大量中性粒细胞等炎性细胞浸润、成纤维细胞和新生血管增生，胶原纤维肿胀、分解等，说明除HSV-1对角膜组织直接破坏外，炎性细胞介导的免疫病理反应在复发性HSK发病机理是重要的因素。

Results The endothelial cell and fibroblast of valves and vessel walls in experimental group were completely decellularized, no cell fragments were retained within the matrix scaffold; collagen fiber and elastin fiber had been preserved with intact structure and wavily arrayed; deoxyribonucleic acid content of valves and vessel walls in experimental group were decreased by 97.58%, 97.25% compared with that of control group.

结果 实验组瓣膜及血管壁中内皮细胞、成纤维细胞去除完全，无细胞核碎片；瓣膜及血管壁胶原纤维和弹力纤维呈波浪状排列、整齐，结构完整；瓣膜、血管壁的基因组脱氧核糖核酸含量分别较对照组下降97.58％和97.25％。

Results T-2 toxin could cause rat articular chondroncytes degeneration, necrosis, and appeared many blank areas free cells. Weigert/Van Gieson staining showed obvious collagen fasciculi emergence could be seen. Scanning electronic microscope showed the surface of articular cartilage was wave and rough. Collagen fasciculi ruptured and stack up, and presented a typical 揳rticular dryness?phenomenon. ELISA results indicated, at the stage of 3 month, the level of rat serum CTX-II in T-2 toxin group was higher than that in control group, and the difference had a markedly significance.

结果 T－2毒素可致大鼠关节软骨细胞变性、坏死，出现大面积无细胞区，胶原染色可见明显的&胶原显现&；扫描电镜显示，关节面起伏不平，表面粗糙，胶原纤维断裂、剥脱，关节表面布满裂片状凸起，呈典型的关节&干燥&现象；ELISA检测结果表明， T-2毒素组大鼠血清CTX-II水平在3个月时较对照组明显增加，差异具有统计学意义。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐