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To address this question in an unbiased and genome-wide manner, we developed a new method, methylation-specific digital karyotyping, and applied it to epithelial and myoepithelial cells, stromal fibroblasts from normal breast tissue, and in situ and invasive breast carcinomas.

为了能无偏向地并在基因组范围解答这个问题,我们开发了一种新的方法——甲基化特异性数字染色体组型,并将这一方法应用于上皮细胞和肌肉上皮细胞、正常乳腺组织来源的基质成纤维原细胞、原位及侵入性乳腺癌细胞。

The forced expression of myogenin could induce NIH3T3 fibroblast to differentiate into myogenesis, it was identified as a pair of bands on SDS-PAGE. The phosphorylated form implies a significant structural change in the myogenin protein, which is expected to affect its function at post-translational level.

强制性表达外源性生肌素可启动NIH3T3成纤维细胞分化,进入成肌过程。pCD1-myog转染后的蛋白印迹带型迁移率发生改变,说明磷酸化导致生肌素蛋白结构的明显变化,并可能在翻译后水平影响其功能。

Skin keratinocytes and epithelial cells from hair follicle organized into epidermoid cyst-like spheroids when cultured on nubby dermal papilla cells gel and epidermoid layer-like structure was formed when they were cultured on free dermal papilla cells and skin fibroblasts gel.

团块状的毛乳头细胞诱导毛囊上皮细胞和皮肤角质形成细胞形成球形结构;而游离分散的毛乳头细胞和皮肤成纤维细胞诱导毛囊上皮细胞和皮肤角质形成细胞形成表皮样层化结构。

When preoxidized fibers are carbonized, with the process of pyrogenation, changes of the structure take place.

在碳化过程中,随着热解过程的进行纤维的结构继续发生变化。

The effects of vacuolization on the differentiation of vascular endothelial cells were analyzed from a chemical biochemistry genetics perspective using the blastodisc cells differentiation model induced by FGF with the γ-butyrolactones derivative.

从化学遗传学的角度,利用γ-丁内酯衍生物研究了成纤维细胞生长因子(fibroblast growth factor, FGF)诱导鸡胚盘细胞向血管内皮细胞分化过程中,空泡化对血管内皮细胞分化的影响。

Effects of reaction temperature, reaction time, the amount of alkali, benzyl chloride and quaternary ammonium on grating rate of benzylated natural bamboo fiber have been discussed.

以接枝率为考察目标用正交试验探索了反应温度,碱用量,反应时间,氯化苄及助剂季铵盐的用量5个因素对天然竹纤维苯甲基化改性的影响,确定了最佳反应条件。

It is found that degree of ring formation, concentration of alkaline solution, time of alkaline treatment have great effects on the mechanical properties of porous hollow gel fibers; the alkali treatment of porous oxidation fiber is easier than solid oxidation fibers.

发现环化程度和碱溶液浓度、碱处理时间对多孔中空凝胶纤维的拉伸行为和力学性能有较大的影响。

The spinnablity of the sols prepared and the performance of the PVA/SiO2 hybrid fibers were researched.

对溶胶的可纺性和杂化纤维的性能进行了研究。

CGRP content in myocardium gradually enhanced from normal group to controls group, and model group's was the highest. We found immunopositive neurons and nerve fibers of nNOS and CGRP by immunochemistry in normal myocardium. They were mainly round-shaped and oval-shaped, also tadpole-shaped、 shuttle-shaped、triangle-shaped、ring-shaped and irregular-shaped. Nv of neurons and nerve fibers in atria were more than in ventricle in some degree; Those in right part of heart were more than in left. Morphology of CGRP neurons changed in model group, the number of ring-shaped neurons increasing, while the round-shaped and oval-shaped decreasing, and Nv and Sv of model group were markedly less than normal. Expression of nNOS mRNA was overly higher than normal group, but CGRP was opposite.

心肌组织CGRP的含量由正常组、实验对照组至CHF组逐渐升高,差异显著;免疫组化研究表明心肌组织中存在nNOS和CGRP免疫阳性神经细胞及纤维,细胞形态多样,正常多以圆形和椭圆形为主,还有梭形、蝌蚪形、三角形、指环形及不规则形,细胞密度心房高于心室,右心高于左心;CHF组CGRP神经细胞形态有一定改变,淡染的指环形细胞增多,而深染圆形或椭圆形细胞减少,其面、数密度均明显低于正常组,差异显著;用RT-PCR测得CHF组nNOS mRNA极度表达,显著高于正常组,而CGRP表达明显低于正常组。

At 24 weeks after the operation, the deep area of the defects was filled with bone tissue, and the surface was covered by hyaline cartilage.

空白对照组:在缺损处未填充任何物质的对照组中,缺损部位主要由纤维组织填充,偶有少量的化生软骨出现。

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