纤维化

Methods Constructed the model of fetal scarless wound healing and investigated the expression of collagen in fetal and adult rat wounds by immunohistochemistry and in situ hybridization.

染色，Ⅰ、Ⅲ型胶原免疫组化，Ⅰ、Ⅲ型胶原原位杂交。结果胎鼠伤口愈合快，组织结构再生完全，没有瘢痕形成，胶原呈网状正常排列，Ⅰ、Ⅲ型胶原蛋白及表达均增多，同时伴细胞数增多，Ⅲ型胶原首先形成网状排列；成年伤口胶原纤维沉积慢，平行排列的胶原逐渐呈束状填塞伤口以致瘢痕形成，伤口内Ⅰ、Ⅲ型前胶原mRNA表达均增多，细胞数目增多不明显。

Immunohistochemically, the new crescent yellowish endings and fibers of trigeminal axon were SP and CGRP radioactive with granular, pearl and reticular delineations, mainly located at the 1rt and 2nd layers of lateral nucleus caudalis spinalis nervi trigemini.

免疫组化结果可见：P物质和降钙素基因相关肽的免疫阳性结构为轴突终末和纤维，呈现颗粒状、串珠状或交织成网状，主要集中分布在三叉神经脊束核尾侧亚核Ⅰ、Ⅱ层内，呈现新月形棕黄色显色。

METHODS: Bone marrow was sterilely separated from human. After heparinization, human BMSCs were harvested using density gradient centrifugation and adherence method. At the fifth passage, BMSCs at 1×108/L were incubated in the 6-well plate and divided into 2 groups. BMSCs in the edaravone group were 50% confluent and incubated in L-DMEM containing basic fibroblast growth factor and fetal bovine serum for 24 hours. After washing in PBS, these BMSCs were incubated in serum-free L-DMEM containing 20 mg/L edaravone for 24 hours. BMSCs in the blank control group were incubated in L-DMEM, supplemented with 10% fetal bovine serum.

无菌抽取的骨髓经肝素化后，采用密度梯度离心法及贴壁筛选法分离获得人骨髓间充质干细胞，传至第5代按1× 108 L-1接种于6孔板内，设立2组，依达拉奉组细胞达50%融合时用含碱性成纤维生长因子、胎牛血清的L-DMEM预诱导24 h，PBS洗涤后再用20 mg/L依达拉奉无血清L-DMEM诱导24 h；空白对照组始终用含体积分数为10%胎牛血清的L-DMEM培养，不加任何预诱导剂和诱导剂。

The central complex isprominent. The beetle showed notable 5-HT-like immunoreactivity throughout the wholebrain, which was also strongly labelled by TPH antibody, with the pattern matching that of5-HT. 5-HT-like positive fibers were resolved in all areas of the brain, among which thelamina and suboesophageal ganglion showed the most remarkable dark brownimmunostaining. While, all major neuropil regions, in general, showed low level backgroundafter TPH immunostaining for TPH mainly existing in cytoplasm. The number of 5-HT-likeimmunoreactive neurons was relatively small but possessed extensive varicose arborizations.

免疫组化结果显示，异色瓢虫脑系统呈强5-HT免疫反应，表现为稳定的反应模式；其脑系统对鼠抗TPH单克隆抗体同样呈显著的免疫阳性反应，且反应模式与5-HT一致。5-HT能神经元多为切向神经元，数目相对较少，呈两侧对称分布，主要位于视叶与侧前脑之间、前脑背方及咽下神经节处，其阳性纤维呈明显的静脉曲张状，投射区域较广泛。

Methods: A rat chronic gastric ulcer model was made by infecting Hp(NCTC11637 strain)after injecting acetic acid into the subserosa of the stomach. In the experiments, the techniques of immunohistochemical stain and in situ hybridization etc. were used to observe the expression of bFGFmRNA and PCNA in th ulcer.

采用幽门螺杆菌多次重复感染乙酸性胃溃疡大鼠，造成Hp相关的慢性胃溃疡模型，运用免疫组化和原位杂交等技术观察该方对Hp感染的慢性胃溃疡大鼠胃黏膜碱性成纤维生长因子mRNA和胃黏膜细胞增殖核抗原的影响。

In the experimental study section, the rats were divided into four groups, including the normal control, the model, the Piracetam, the QPC, and copied to AD model. After the treatment, we observed the effects on the AD rats' ability of leaning and memorizing, detecting the TChE activity in whole blood and the expression levels of the related biochemical indicators of As includeing GFAP、NGF and BDNF of the AD rats' brain hippocampus, then compared the QPC with other groups to reveal the mechanism of action of the QPC treating AD to bright.

在动物实验部分，我们通过塑造AD动物模型，设立正常对照组、模型组、脑复康组和强脾益智胶囊组，观察强脾益智胶囊对AD模型大鼠空间学习记忆能力的影响，检测AD大鼠全血、海马匀浆液中乙酰胆碱酯酶活性和通过免疫组化的方法检测胶质纤维酸性蛋白、神经生长因子、和脑源性神经营养因子的表达情况来反映星形胶质细胞的增值情况，以期揭示强脾益智胶囊对AD的治疗作用机理。

The invention provides a method for preparing an epoxy sheet-like mold plastic, which comprises the following steps: thickening the mixed epoxide resin system with diisocyanate compound; strengthening the chopped strand; making the tectorial into non-stick hand and shear epoxy sheet-like mold plastic, that is, preparing the epoxide resin paste and the epoxy sheet-like mold plastic; ripening.

本发明提供的制备环氧片状模塑料的方法是：采用二元异氰酸酯化合物对混合环氧树脂体系增稠，短切纤维增强，覆膜制备成不粘手可剪切的环氧片状模塑料；该方法包括环氧树脂糊的制备、环氧片状模塑料的制备和熟化步骤。

The results also show that Kevlar composite laminates performs stronger anti-penetration ability than steel targets, and the torqued rods have stronger penetration performance than non-torqued rods.

计算结果表明Kevlar增强纤维复合材料由于其比强度高而具有更好的抗侵彻贯穿性能，预扭转钨合金杆弹由于侵彻过程中弹头较未扭转弹更易于锐化，即出现所谓的自锐现象，因而具有更高的侵彻能力。

On day 3, 7, 15, 30 and 90, renal biopsies were performed. Renal histology was checked under light microscopy with HE and Masson's trichrome staining sections.

在肾炎不同时间点(3、7、15、30和90 d)行肾活检，HE和Masson's染色，光镜观察肾组织形态，免疫组化ABC法检测Mф、纤维连接蛋白、Ⅲ和Ⅳ型胶原。

Whereas osteoporosis is unassociated with bone pain, osteomalacia has been associated with isolated or generalized bone pain.39,40 The cause is thought to be hydration of the demineralized gelatin matrix beneath the periosteum; the hydrated matrix pushes outward on the periosteum, causing throbbing, aching pain.7 Osteomalacia can often be diagnosed by using moderate force to press the thumb on the sternum or anterior tibia, which can elicit bone pain.7,40 One study showed that 93% of persons 10 to 65 years of age who were admitted to a hospital emergency department with muscle aches and bone pain and who had a wide ariety of diagnoses, including fibromyalgia, chronic fatigue syndrome, and depression, were deficient in itamin D.41

然而，骨质疏松症与骨痛无关联，而软骨病则与局部性或全身性骨痛有关。其原因被认为是骨膜下已去矿质化的胶原基质上发生的水合反应，水合的胶原基质在骨膜上向外扩张，引起阵痛。软骨病可通过以拇指适度挤压胸骨和胫骨前方以引起骨痛感。一项研究显示10岁到65岁中有93%的人向医院急诊室承认有肌肉疼痛和骨痛症状，他们还有一些其他症状包括纤维肌痛、慢性疲劳综合征、抑郁等，该研究显示他们都缺乏维生素D。本人已认领该文第9、10两部分，48小时后若未提交译文，请其他战友自由认领。本人已认领该文11、12部分编译，48小时后若未提交译文，请其他战友自由认领。本人认领第十三部分，48小时内交稿请战友纠错！

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。