纤维化

Methods MTT colorimetry,a silver colloid technique to identify nucleolar organizer regions and immunohistochemical methods were used to study the effects of the two inhibitors on growth of fibroblasts.

用MTT比色、AgNORs银染和免疫组化技术，观察两种抑制剂对成纤维细胞增殖、AgNORs合成和Ⅲ型胶原表达的影响。

Methods: Immunohistochemical study of CD133 expression: 55 cases osteosarcoma conventional paraffin-embedded tissues specimens come from Pathology Department Qilu Hospital Shandong University, including 4 cases parosteal osteosarcoma, 13 cases fibroblast-type osteosarcoma , 12 cases chondroblastic osteosarcoma and 26 cases Osteoblastoma osteosarcoma.

免疫组化研究CD133的表达：取自本院病理科的骨肉瘤常规石蜡包埋组织蜡块标本55例，其中骨旁骨肉瘤4例，纤维母细胞型骨肉瘤13例，软骨母细胞型骨肉瘤12例，骨母细胞型骨肉瘤26例，切成3μm的组织切片备用。

The periostea of both experimental and control side of the mandibular ramus were taken and prepared, 2 of each 5 rabbits in a group were prepared for HE stain detection and 3 for proliferating cell nuclear antigen immunohistochemical detection.Results:1, The newly formed bone was detected on the lateral aspect of mandibular ramus after periosteal distraction. The bone was shaped like a hill. It looked very low and was full of holes at postoperative day 28. With the time of consolidation period lengthened, the newly formed bone matured gradually. X-ray examination showed the new bone shaped like a hill. The average values of new bone height at postoperative days 28,35,42 and 56 were 1.86 + 0.15mm, 2.29 + 0.29mm,3.19 + 0.13mm and 4.70 + 0.45mm. Histological examination of both HE stain and picricacid-fuchsin stain showed the increase in the number of osteoblasts and the change in the orientation of collagen fibers and bone trabecula. There were no significant differences between newly formed bone and original bone on the lateral aspect of mandibular ramus at postoperative day 56 histologically.2 Compared with the control side, the distracted periostea proliferated obviously under the microscope, and the number of periostealcells increased with satiation of cellular nuclear per unit area. The images of PCNA immunohistochemical stain of periosteum showed that the experimental periosteum proliferated obviously after distraction compared with the control side.

结果：骨膜牵张成骨的实验研究南京医科大学硕{学位论文l、骨膜牵张后，可见下领升支外侧的骨皮质上有新骨形成，新骨呈山峰状凸起，术后第28天的新生骨较低平，多孔隙，随着固定时间的延长，新骨逐渐成熟；下领升支前后向切线位X线投照显示新骨呈山峰样隆起；经测量，术后第28、35、42和56天组平均新生骨厚度分别为x.86士0.15mm、2.29士0.29mm、3.19士0.13mm和4.70 土0.45mm；脱钙骨组织的HE染色和不脱钙骨组织的苦味酸一品红染色的组织学观察均显示了新生骨在成骨细胞数量上的增长，以及胶原纤维和骨小梁排列方向上的变化，术后第56天的新生骨在组织学上与原升支骨组织已无明显区别。2、HE染色显示，与对照组相比较，实验侧骨膜增生明显，细胞间排列紧密，单位面积内骨膜细胞数增多，细胞核饱满；骨膜PCNA 免疫组化染色显示，与对照侧相比较，实验侧骨膜在牵张后出现了明显的增生迹象，PCNA阳性细胞分布紧密，单位面积内阳性细胞数较对照组多，靠近骨表面的骨膜中的阳性细胞数更多而且分布更为紧密。

Proliferative retinopathy is a series ofretinal diseases,including diabetic retinopathy,retinopathy of prematurity,central retinal vein occlussion and retinal periphlebitis,and so on,characterized as neovascularization,recurrent vitreous hemorrhage,proliferation and even traction retinal detachment and retinal atrophy. It causes the patients serious vision damage even getting blind.

目的： 新生血管增殖性视网膜病变，是一组严重的视网膜病变，其晚期主要表现是大量视网膜新生血管形成，引起玻璃体反复出血、机化和纤维增殖，甚至发展为牵引性视网膜脱离或视网膜萎缩，导致视力严重下降进而完全失明。

MethodsThe transplanted tumor model of the mouse S180 sarcoma was established.Fifty mice were randomly divided into five groups,the control group,the CTX group,the Gecko group.They were treated respectively with oral administration of saline,and intraperitoneal injection of CTX 100 mg/kg only one time,oral administration of Gecko in doses of 13.5,9,4.5 g/kg,one time a day.After 14 days,the anti-tumor activity was evaluated by tumor tissue weighing. The impact on immune organ was detected by accounting thymus index, spleen index and the number of peritoneal macrophage which phagocytose chicken red blood cells.The protein express of vascular endothelin growth factor and basic fibroblast growth factor were detected by SABC immunohistochemistry.

方法建立移植瘤小鼠S180肉瘤模型，将50只雌鼠随机分为对照组、环磷酰胺组、壁虎高组、中组、低组共5组，分别给于生理盐水灌胃1次/d，CTX（100 mg/kg）腹腔注射1次，壁虎高、中、低组（13.5，9，4.5 g/kg），每天灌胃1次。14 d后，称取荷瘤小鼠瘤重、胸腺重、脾脏重，计算抑瘤率、胸腺指数、脾脏指数，观察腹腔巨噬细胞吞噬鸡血红细胞实验指标；用SABC免疫组化法检测血管内皮生长因子、碱性成纤维细胞生长因子的蛋白表达，TUNEL方法检测细胞凋亡率。

The therapeutic mechanism of the method of nourishing qi, activating blood, dispersing phlegm and promoting the collaterals was supposed as that it intervened the airway remodeling and alleviated the progress of airflow obstruction of COPD by suppressing the chemo taxis of cytokines, regulating the proliferation of fibroblast cell and smooth muscle cell and preventing the proliferation and plerosis of extracellular matrix.

推测益气活血化痰通络法防治COPD的机制可能为：通过抑制炎症细胞的趋化、影响细胞因子的活性、调节成纤维细胞和平滑肌细胞的增殖、阻止细胞外基质的增生修复，从而达到干预COPD患者气道结构重塑，减缓COPD气流受限进展的作用。

Methods OA was injected into hippocampal CA1 region of rats. We observed rat praxiology changes by Morris water maze and the senile plaques and neurobrillary tangles of hippocampus by Bielschowsky stain and the expression of β-amyloid protein and neuronal nitric oxide synthase of the rats hippocampus by immunohistochemistry.

在大鼠海马CA1区多次微量注射OA，水迷宫实验观测大鼠行为学改变；Bielschowsky染色观察海马CA1区神经原纤维缠结和老年斑等特征性病理变化；免疫组化方法观察海马CA1区Aβ1-40和nNOS的表达。

A very small number of double-positive cells expressed with PCNA and glial fibrillary acidic protein were first detected in infract boundary in preoptic area on day 3 and onward.

免疫组化双标显示再灌流3 d视前区有很少量的增殖性细胞核抗原/胶质纤维酸性蛋白双阳性细胞，随后增多。

The reverse transcription-polymerase chain reaction was used to seek the expressions of a specific fibroblast marker, prolyl 4-hydroxylase β, and a specific marker of epithelial cells, cytokeratin-4, in the cells.

采用逆转录多聚酶链反应检测hEFs表达成纤维细胞特异性标志（脯氨酰4-羟化酶β亚单位）和上皮细胞特异性标志（细胞角蛋白4）的情况，对该细胞进行鉴定。

When preoxidized fibers are carbonized, with the process of pyrogenation, changes of the structure take place.

在碳化过程中，随着热解过程的进行纤维的结构继续发生变化。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。