纤毛

The ciliature microtubular organelles in the ventral cortex of Urostyla grandis were visualized by direct fluorescent labeling of FLUTAX .The organelles consist of adoral zone of membranelles, undulating membranes,frontal-midventral-transverse cirri,left and right marginal cirriand the base-associated microtubules,etc.

应用荧光紫杉醇直接荧光标记显示，腹毛目纤毛虫大尾柱虫 Urostyla grandis 腹皮层纤毛器微管胞器由口围带、波动膜、额腹横棘毛和左、右缘棘毛等纤毛器微管、纤毛器基部附属微管等组成。

Results There is egg cover before insect egg and is wrinkled increase part behind it. The nipples having cilium,sanken having or not cilium and little holes are distributed symmetrically around the oral sucker of cercariae. The nipples having cilium are discovered in the rest,which may have sense function.

结果 虫卵前端有卵盖、末端有皱褶增厚部分；尾蚴的口吸盘周围对称分布着有纤毛的乳突，有或无纤毛的凹陷及小孔；具纤毛的乳突在虫体的其余部分也有发现，可能起感觉器的作用。

In resting cysts, the microtubules under the pellicle, cortical ciliatures, conjunctions between cilia basal bodies, supporting structure of basal bodies and other microtubule structures are absorbed in different extent; microtubule-like assemble bodies appear at the center of the remained cilia basal bodies; the granules of chromatin and nucleolus in macronucleus become smaller whereas nucleus pores of the micronucleus become bigger; the inner membrane of some nucleus pores are attached with chromatin.

结果表明：纤毛虫形成包囊过程中，皮层细胞质中产生具有自噬泡消化特征的细胞器，将部分纤毛结构等逐步消化；休眠包囊中，表膜下微管、皮层纤毛器和纤毛基体间连接、基体托架及其他微管结构不同程度地被吸收、在残剩纤毛基体的中央形成微管形结构聚合体，大核染色质和核仁颗粒变小，核孔增大，并发生染色质附着于核孔内膜的现象；细胞脱包囊期间，皮层细胞骨架、线粒体等经历了在残剩结构基础上的再分化。

Results Ciliary beat frequency of cultured human nasal epithelial cells is 10.39±1.80 Hz at 25℃. Ciliary beat frequency of different cilia is equal on the same cell, it is also equal from uncinate process and inferior turbinate.

结果 在25℃，体外培养的人鼻腔黏膜纤毛摆动频率是10.39±1.80Hz；同一细胞任意两点纤毛摆动频率是相同的；同一来源的体外培养的钩突和下鼻甲的纤毛摆动频率是相同的。

Ciliary beat frequency was measured by digital high-speed microscope. results ciliary beat frequency of cultured human nasal epithelial cells is 10.39±1.80 hz at 25℃. ciliary beat frequency of different cilia is equal on the same cell,it is also equal from uncinate process and inferior turbinate.

结果 在25℃，体外培养的人鼻腔黏膜纤毛摆动频率是10.39±1.80hz；同一细胞任意两点纤毛摆动频率是相同的；同一来源的体外培养的钩突和下鼻甲的纤毛摆动频率是相同的。

By phenotype observation, combined with root tipchromosome counting, PMC MI pairing analysis and chromosome C-banding, a ditelosomic addition line 94K227 and a disomic addition line 94K280 was identified among BC2F2 and BC2F3 population respectively.C-banding results showed that 94K227 contains 1 pair of long arms in chromosome B, and 94K280 contains 1 pair of chromosome D from R.

用普通小麦-纤毛鹅观草双倍体与普通小麦中国春连续回交两次，然后连续自交，通过形态观察、根尖细胞染色体计数、花粉母细胞减数分裂中期Ⅰ染色体配对分析及染色体C－分带，在BC2F3和BC2F3群体中，分别筛选到一个端二体异附加系94K227和一个二体异附加系94K280，其中C-分带显示94K227添加的是纤毛鹅观草染色体B的一对长臂， 94K280添加的是纤毛鹅观草的一对染色体D。

To investigate the presence of specific adhesion activity, the mrkD adhesin gene of the established type 3 fimbriae expression system was replaced respectively by each of the fimbrial adhesins. Transmission electron microscopy and western blot analysis revealed that most of the recombinant type 3 fimbriae expressed and assembled properly.

位於纤毛末端的黏附蛋白为决定细菌黏附专一性的主要因子，为了测试黏附蛋白的活性，我们进一步以过去建立的第三型纤毛表现系统为平台，将MrkD黏附蛋白基因分别置换为kpa、kpb、kpc、kpd、kpe、kpf或kpg的黏附蛋白基因，并经西方墨点法确认这些重组第三型纤毛的结构蛋白的表现和组装正常。

In the genome of Klebsiella pneumoniae NTUH-K2044, we found nine fimbrial gene cluters including fim, mrk, and seven novel fimbrial operons, namely kpa, kpb, kpc, kpd, kpe, kpf, and kpg. These fimbrial gene clusters were cloned into the expression plasmid pET30b and then transformed to an afimbriated E.

在克雷白氏肺炎杆菌NTUH-K2044的基因体中，藉由HMMER找到了九套纤毛基因组，其中包含已知的第一型纤毛fim基因组和第三型纤毛mrk基因组，以及七套未曾被报导的纤毛基因组，分别被命名为kpa、kpb、kpc、kpd、kpe、kpf和kpg。

The epithelia of both mucocele and nasal mucosa is the same pseudostratified ciliated columnar epithelium, rich cilium in mucocele and the function of cilium could recover.

鼻窦黏液囊肿内衬上皮与鼻腔大部分黏膜一样均为假复层纤毛柱状上皮，纤毛发达，术后纤毛功能可以恢复，其超微结构与病理特性密切相关。

Abstract］ Objective To investigate the change of bronchial mucosa cilia epithelium structure on children with recurrent respiratory tract infection in acute stage and convalescence stage.Methods Choose 38 cases of patients with RRTI as study group,respiratory ciliated cells were collected by bronchial fiberscope in acute stage and convalescence stage respectively,observe the change of cilia structure by electron microscopy and compare the incidence of cilia cylindrical epithelium structure abnormality.

摘要］ 目的研究反复呼吸道感染患儿急性期与恢复期支气管黏膜纤毛上皮结构的特点与变化，临床治疗的有效性提供客观依据方法选择符合儿童RRTI诊断标准的患儿38例作为研究对象，分别于急性期及恢复期在纤维支气管镜下夹取支气管黏膜组织，电镜下观察纤毛上皮结构的特点并比较纤毛异常的发病率，探讨RRTI患儿治疗后纤毛的恢复情况。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。