红豆草

Theory basis were given to physiology study of seed development, production and utilization of sainfoin.

为红豆草种子发育生理研究及其生产利用提供理论依据。

The circumtances of the protolasts culture of leguminosae and the tisse and protoplasts culture of sainfoin has been reviewed in this paper.

本文还综述了豆科原生质体和红豆草原生质体的培养概况以及影响豆科原生质体培养的几个重要因素。

The growth periods of sainfoin and A. adsurgens prolonged, some inflorescences of sainfoin returning green in SP1 plants.

红豆草和沙打旺当代植株生长期延长，红豆草的处理组部分植株的花序出现花序返青现象。

Sainfoin ; Tissue culture ; Somatic embryo ; Cloning

红豆草；组织培养；体细胞胚；克隆

Seedlings survived at 1. 5% NaCl was obtained from SP2 seeds and used as explants for tissue culture. A stable NaCl-tolerant cell line of sainfoin was selected by multi-step selections and also showed resistance to PEG stress.

自搭载后的红豆草第二代种子开始，得到能在1.5％NaCl上存活的幼苗，以此幼苗为外植体，通过组织培养筛选，得到耐盐系，耐盐系还表现出对PEG的交叉抗性。

RAPD method was used to determine the molecular nature of the variants, and polymorphism, such as OPD05-1250 and OPH16-800 in NaCl-tolerant line of sainfoin and OPA08-1180 and OPH01-760 in ethionine-resistant cell line alfalfa, could be clearly detected among different lines, indicating these variations were caused by the changes in DNA.

用RAPD技术对红豆草和苜蓿几种变异系的DNA多态性进行比较分析，证实红豆草耐盐系中具有OPD05-1250、OPH16-800等多态性产物，苜蓿变异系中具有OPH01-760、OPA08-1180多态性产物。说明变异系中DNA水平发生一定改变。

Grass that is adapted to Qinghai-Tibet Plateau are: caragana, sand sagebrush, lucerne, sweet clover, sudan grass, ryegrass, lyme grass, astragulus adsurgens, puccinellia tenuiflora, bromus inermis, agropyron elongatum, harmel, siberian wildrye , sainfoin, tall fescue , red fescue, cynodon dactylon, zoysia, clover, bluegrass, ryegrass etc..

适合我们青海高原种植的草有：柠条、沙蒿、紫花苜蓿、草木犀、苏丹草、黑麦草、披碱草、沙打旺、星星草、无芒雀麦、冰草、骆驼蓬、老芒麦、红豆草、高羊茅、紫羊茅、狗芽根、结缕草、三叶草、早熟禾、黑麦草等；适合种植的树木品种有：雪松、青海云杉、侧柏、祁连圆柏、速生杨、落叶杉、新疆杨、青海榆树、红柳、垂榆、刺槐、丁香、云杉、沙棘、枸杞等。

With vertical-section analysis, in the later of experiment, naked terra and wheat land make content of soluble salt high in 0～20cm,and decreasing with deepening of soil-layer; the content value of planting two-year-old Onobrychis viciaefolia 、one-year-old Onobrychis viciaefolia 、interplanting and sequential cropping in the every soil-layer is equal; in harvest, only planting wheat and interplanting display content of soluble salt is high in middle-layer (20～60cm) and low in surface and down layers(0～20cm,60～80cm).

土壤垂直剖面分析，试验末期裸地的土壤盐分表现为0～20cm 土层高，随土层深入含量逐渐降低；二年生红豆草、一年生红豆草、套种和复种处理的土壤盐分均表现出各层含量近似相等的特征；小麦收获期，单作小麦和小麦套种地各土层的可溶性盐含量均表现为中层(20～60cm)高、上下层(0～20cm 和60～80cm)低的特征。

From Apr.1st to Oct. ninth, when two-year-old Onobrychis viciaefolia 、one-year-old Onobrychis viciaefolia 、Triticum 、Triticum aestivum/ Vicia villosa 、Triticum aestivum/ Brassica campertris 、Triticum aestivum-Vicia villosa and Triticum aestivum-Brassica campertris are planted, their content of soluble salt in 0～20cm soil layer are lower than that in uncovered soil, in the period of harvest , they decrease 0.361%、0.331%、0.141%、0.327%、0.312% and 0.317% respectively.

从时间变化分析，采用二年生红豆草、一年生红豆草、单作小麦、小麦/毛苕子、小麦/油菜、小麦-毛苕子和小麦-油菜7 种绿色覆盖模式时均使土壤表层盐分含量比同期裸地的低，生育期末分别降低了0.361%、0.331%、0.141%、0.327%、0.312%、0.321%和0.317%。

Paper electrophoresis showed that the Onobrychis viciaefolia and Medicago sativa cell line transformed with A〓 synthesized agropine and mannopine, and Onobrychis viciaefolia cell line transformed with LBA9402 Bin19 only synthesized agropine.

红豆草转化系获得再生植株。LBA9402 Bin19转化系可以抗100mg／l卡那霉素。苜蓿A4转化系未能得到再生植株。纸电泳分析表明红豆草A4转化系和苜蓿A〓转化系可以合成农杆碱和甘露碱，而红豆草LBA9402Bin19转化系只能合成农杆碱。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。