红豆杉醇

The results showed that the addition of the rare earth compound or the organic solvents could strengthen obviously taxol release, specially the organic solvents.

结果表明在红豆杉细胞悬浮培养中，分别加入稀土化合物和有机溶剂都明显促进紫杉醇的释放，特别是有机溶剂更显著促进紫杉醇的释放。

The regulation of taxol biosynthesis is one of the key techniques to produce taxol by cell culture.

紫杉醇生物合成调控是实现红豆杉细胞培养生产紫杉醇的关键技术之一。

Based on the detailed analysis of a variety of factors affecting stability of taxol production in Taxus cells, it was discovered that initial cell density and the relevant concentrations of substrates, elicitors and precursors significantly affected stability of taxol production; farther study showed that addition of elicitors at appropriate cellular state was the key to steadily improve taxol yield, especially, the proper cellular state was at the end of lag phase and at the beginning of the stationary phase, which was also proved by the cellular ultrastructure.

对影响红豆杉细胞紫杉醇产量稳定性的各类因素进行详细分析，发现细胞的接种密度以及与之相适应的培养基的基质浓度、诱导子浓度和前体浓度是影响紫杉醇产量稳定的重要因素；进一步研究发现，选择细胞处于合适的状态时加入诱导子是稳定提高紫杉醇产量的关键，细胞合适状态特别是延迟期末和稳定期初最合适，细胞状态的超微结构观察结果也证明了这一点；同时研究发现采用合适的同步化处理获得的细胞初始周期时相加入诱导子与紫杉醇产量稳定密切相关，且以低温同步化处理得到的细胞周期的分裂中期相的细胞加入诱导子不仅大大提高了紫杉醇的产量，而且诱导子加入时间大大缩短，有利于大大提高紫杉醇生产率。

The results showed that precursor feeding (phenylanine, sodium acetate and benzamide) and mixed sugar as production medium resulted in significant increase of taxol production not only in the one-liquid-phase cultures of Taxus chinensis var. mairei but also in the two-liquid-phase cultures. And the integrated function of precursor feeding and mixed sugar in the two-liquid-phase cultures gave more significant increase of taxol production significant increase of taxol production, which was four times more than that of the control (the one-liquid-phase culture without precursors and mixed sugar).

结果表明前体饲喂（苯丙氨酸，醋酸钠和苯甲酰胺）和蔗糖与麦芽糖配伍作为生产培养基糖源不仅对红豆杉细胞悬浮培养，而且对其两液相培养的紫杉醇产量提高均有明显的促进作用，与对照组相比，在南方红豆杉细胞两液相培养中，采用前体饲喂和蔗糖与麦芽糖配伍作为生产培养基糖源，紫杉醇产量提高4倍多。

Cuspidate with 33％ identity and 50％ similarity to taxadien-5α-ol-O-acetyltransferase, 34％ identity and 52％ similarity to 10-deacetylbaceatin Ⅲ-10-O-acetyltransferase, and 33％ identity and 49％ similarity to taxane 2α-O-benzoyltransferase, respectively, moreover, it contained the conserved HXXXDG motif that is found in other transacylases, this sequence element has been suggested to function in acyl group transfer from acyl CoA to the substrate alcohol.

No.AF448807）在氨基酸水平与大部分植物来源的酰基转移酶具有较大的同源性，其中与东北红豆杉紫杉二烯-5α-醇-乙酰转移酶的一致性达33％、相似性达50％，与东北红豆杉紫杉10-去乙酰巴卡亭Ⅲ-10-0-乙酰转移酶的一致性达34％、相似性达52％，与东北红豆杉紫杉二烯2α-0-苯甲酰转移酶的一致性达33％、相似性达49％，并且在TS4的氨基酸序列中还发现酰基转移酶家族成员特有保守序列HXXXDG，这个序列单元被认为在将酰基CoA转移至醇类底物的过程中发挥作用。

Effects of ABT rooting powder No.1 (ABT1),α-Naphthaleneacetic acid and Indolebutyric acid on the rooting rates, biomass and taxol contents of Taxus chinensis var. mairei cuttings were studied in this experiment.

以清水处理为对照，使用不同激素(ABT1号生根粉、NAA、IBA)处理南方红豆杉插穗，从而研究不同激素处理对南方红豆杉扦插苗生根率、生物量及紫杉醇含量的影响。

The result showed that sodium orthovanadate had a increasing effect on the paclitaxel content of cell cultures.

结果表明，钒酸钠对红豆杉悬浮培养的紫杉醇合成有明显的促进作用，添加50 μmol/L的钒酸钠使细胞的紫杉醇含量增加了71.3％。

The invention relates to a new strain producing taxol and a taxol preparation method. The taxol is a diterpenoid alkaloid drug which is extracted from taxaceae plants and has a plurality of anti-tumor effects. Taxus, also known as yew, is a prehistoric plant with a history of 0.2 billion years and is a rare plant species with low growing speed; the biological characteristics of the taxus have high demand on the ecological environment; the natural renewal ability is poor, so the amount of the species is very limited, belonging to an endangered protection plant with limited resource, thus, scientists at home and abroad actively research and develop new taxol sources. The new strain for producing the taxol of the invention is the strain which is first separated from the bark of T.cuspidate Sieb.

产紫杉醇的新菌种及其选育和制备紫杉醇的方法，紫杉醇是从红豆杉属植物中提取出来的具有多种抗肿瘤疗效的二萜生物碱类药物，由于红豆杉，又名紫杉，是两亿年前的史前植物，是珍稀树种，生长速度缓慢，并且其生物学特性对生态环境要求较高，天然更新能力差，所以种群数量极为有限，属濒危保护植物，资源十分有限，因此，国内外学者都在积极地研究开发紫杉醇的新药源，本发明产紫杉醇的新菌种，所述的新菌种为从东北红豆杉T.cuspidate Sieb。

Mairei is a variation of Taxaceae Taxus in china, and it containsPaclitaxel, which is a kind of anticarcinogen, in its branch, bark, needle, root andpulp. In order to production oftaxol in callus cultures of Taxus chinensis var.

mairei，为红豆杉科红豆杉属在中国的1个变种，其枝、皮、叶、根和果肉中均含有抗癌药物紫杉醇(Paclitaxel，商品名Taxol)。

Company's main business areas are yew artificial plant and paclitaxel anti-cancer drugs developed series development and production of sales, is currently the only company to have both the production of paclitaxel drug approval [State××X19990268] and paclitaxel injection production approval [Sinopharm quasi - word X19990269] the enterprise, is the first by the national GAP standard building large-scale cultivation of yew-based enterprises.

公司主营业务是红豆杉的人工种植和紫杉醇抗癌系列药品的研制开发与生产销售，是国内目前唯一一家同时拥有紫杉醇原料药生产批文[国药准字X19990268]和紫杉醇针剂生产批文[国药准字X19990269]的企业，也是目前全国首家按GAP标准建设的红豆杉规模化栽培基地的企业。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。