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Materials and Methods: Campare gasless hand-assisted laparoscopic surgery, carbon dioxide pneumoperitoneum laparoscopic surgery and operation on Cholecystectomy, colorectal resections, according to the study of operation time, expense in hospital, number of days, pathologic type, extension of radical cure, and analysis of the blood lymphocytes, immune function of erythrocyte, we could ascertain the application value of gasless hand-assisted laparoscopic surgery.

材料与方法:对比研究非气腹手助腹腔镜手术、CO_2气腹腹腔镜手术及开腹手术,从早期的胆囊切除手术的临床应用过渡到结直肠癌根治手术,分别从手术时间、住院费用、住院天数、病理分型、根治范围等进行对比研究,然后从人体外周血淋巴细胞、红细胞等免疫功能方面作进一步分析,以确定非气腹手助腹腔镜手术在临床中的应用价值。

Compared with acute-phase reaction and erythrocytic immune function, neutrophilic counts, interleukin-6, natural killer cells, C-reaction protein between gasless hand-assisted laparoscopic surgery and carbon dioxide pneumoperitoneum laparoscopic surgery, we found there were obvious difference on N/L rate, CRP, IL-6, Which provided evidence for evaluate the gasless hand-assisted laparoscopic technique superiority to the traditional surgery, and treatment for clinical immune modulation.

通过非气腹手助腹腔镜、CO2气腹腹腔镜和开腹手术创伤后出现的急性期反应和红细胞免疫功能的检测,中性粒细胞计数、IL-6,NK,CRP浓度等APR指标检测,发现非气腹手助腹腔镜结直肠癌手术术后中性粒细胞/淋巴细胞、CRP、IL-6等免疫指标的变化情况较腹腔镜组、开腹组有明显区别,为评价此手术技术是否较传统方法优越和临床免疫调节治疗提供依据。

And the effect of the percentage of hydrochloric acid to acetone,the volume ratio of hydrochloric acid acetone to hematolysis solution were researched.Results:The supersonic wave hemolysis was Established.

结果:确立超声波法进行红细胞溶血,36.5%浓盐酸与丙酮的体积为1%的血红素抽提液,与溶血液的体积比为5∶1抽提10分钟,最终可从新鲜猪血中得到红素6.0 g/L,纯度达99.8%。

They could be fitted by experience formulas as follows:For PVP: Dl =and for PBS.where0 is the initial value of DI, t0, t1 and t2 are characteristic time of non-isochoric deformation, isochoric deformation and orientation of erythrocyte respectively.

结果表明,红细胞在低粘介质中的动力学行为和它在高粘介质中的动力学响应有显著差异,除了等容变形和非等容变形以外,还有一个从定向到随机取向的变化过程。

Methods: Using chemiluminescence assay to determine phagocytic function of celiac macrophagocyte and the erythrocyte immunization of the mouse model by reserpine.

采用化学发光测定和分析的方法,对利血平造模的小鼠进行腹腔巨噬细胞吞噬功能和红细胞免疫功能的测定。

Methods The experimental mouse was injected with high, middle, and low dosages of marasmius androsaceus po1ysaccharide 5, 10, 20 mg kg^(-1d^(-1) in the abdomen, and the comparison group was given the same volume of saline water i. p. once a day. After treatment for 14 consecutive days, the blood cell count instrument was used to measure the mice hemogram, the phagocytic function of peritoneal macrophage and the ability of carbon granular clearance were observed., the ability of the spleen lymphocytes proliferation was tested by MTT, and the hemolysin production test was used to evaluate the influence of MA polysaccharide on specific humoral immunity in mice.

小鼠腹腔注射不同剂量的鬼毛针多糖5, 10, 20 mgkg^(-1d^(-1),对照组给予等量生理盐水,每天给药1次,连续14d后,用血细胞计数仪检测小鼠血象;用腹腔巨噬细胞吞噬鸡红细胞实验、碳粒廓清实验观察小鼠吞噬细胞的吞噬能力;采用MTT法测定小鼠脾T淋巴细胞增殖能力;采用血清溶血素形成实验观察鬼毛针多糖对小鼠特异性体液免疫功能的影响。

Lineage-tracing analyses indicate that the ectopic endothelial cells in embryos with decreased Notch activity originate from mesodermal cells that normally produce erythrocyte progenitors.

命运追踪结果显示,Notch降低的胚胎中,异常增加的内皮细胞是来源于本应该形成红细胞前体的中胚层细胞。

The micronuclear frequencies of fetal liver blood were 2.67, 3.33 and 4‰ in treatment group, given intraperitoneally combined NET-OEN at doses of 10, 50 and 100mg/kg, respectively. However, the micronuclear frequencies of bone marrow erythroblasts and fetal liver blood in control group were 1.8~3‰.

作者采用微核试验观察了复方庚炔诺酮对小鼠活体骨髓多染性红细胞及胎肝血微核率的影响,检测其有无潜在的致突变作用,从而为评价复方庚炔诺酮的安全性提供依据。

Conclusions】 It is suggested intramuscular injection is an effective immune route. Mice inoculated with coding different stage gene recombinants alone or mixedly could all induce increased humoral and cellular immune response, and NK cell activity.

提示肌肉注射为一有效的DNA疫苗免疫途径,采用编码恶性疟原虫有性期阶段或无性红细胞阶段的重组质粒单独或两者混合免疫小鼠,均能诱导明显的体液免疫反应、细胞免疫反应和NK细胞杀伤活性。

Depositing cells were incubated in high-sugar DMEM culture medium containing 15% fetal bovine serum, 100 U/mL penicillin, and 100 U/mL Streptomycin, then cells were seeded in a 25 T culture bottle after cell density was adjusted to 1×109 L-1, and incubated at 37 ℃, 0.05 volume fraction CO2 with saturated humidity. After 72 hours, the culture medium was replaced, and red blood cells and other nonadherent cells were removed. Cells began to passage at 70%-80% confluence.

实验方法:穿刺抽取15 mL脐带血,密度梯度法分离吸取分层界面的白色环形絮状物,其中富含单个核细胞,离心后沉积细胞用含15%胎牛血清、100 U/mL青霉素、100 U/mL链霉素的DMEM高糖培养基悬浮,调整细胞密度为1×109 L-1接种于25 T培养瓶内,放置在37 ℃、体积分数为0.05的CO2饱和湿度培养箱中培养,72 h后更换培养基,去除红细胞及其他未贴壁细胞,至70%~80%融合时传代。

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