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The amount of vimentin IF per cell was higher than those of tubulin and F-actin at 12-24h of culture, but it broken down and decreased steadily when the cells became differentiated into late erythroblast at 36-48h of culture, thus facilitated the eccentric nucleation which we regard it as the initial step of denucleation. Both of the fluorescence intensity of tubulin and actin exhibited a significant rise and aggregated between the extruding nucleus and incipient reticulocyte before and during denucleation, and finally play a role in the commitent to ennucleation, the second phase of denucleation.

在培养24小时前,每个细胞中波形纤维含量均高于同期微丝和微管蛋白含量,但在培养24-36小时的晚幼红细胞阶段,波形纤维蛋白降减和解聚,为核偏位创造了条件,这是排核的第一阶段,而随后微丝和微管蛋白的荧光染色强度则有所增加,并逐渐聚集在外排中的核与预期形成网织红细胞之间的缢痕区,在将细胞核排出的排核第二阶段起主要作用。

These changes occur in many tissues but are best known in the precursors of red cells in the bone marrow which is said to exhibit megaloblastic erythroblastosis. In addition to nuclear enlargement in megaloblasts there is increased amount of cytoplasm, cytoplasmic basophilia due to excessive RNA and premature haemoglobinisation as judged by the immature state of the nucleus.

这些变化出现于许多组织,但在巨成红细胞生成的骨髓红细胞前身中最好识别,巨成红细胞除核增大外,胞浆量增加,由于RNA过多致胞浆呈嗜碱性和早熟的血红蛋白形成,这是根据其核的不成熟状态而作出的判断。

The results indicated that in the FNAC smears of mediastinal tumor, neutropilic myelocyte, neutropilic metamyelocyte, neutropilic stab granulocyte and neutropilic segmented granulocyte were observed in granulocytic series; early normoblast, polychromatic normoblast and orthochromatic normoblast were observed in erythrocytic series; and granular megakaryocyte, thromocytogenic megakaryocyte, scattered platelet and matured lymphocyte also were observed. Cell morphology and distribution in different stages were are similar to that in bone marrow smear, and these cells belong to the extra-medullary hemopoietic tissue.

结果表明:纵隔肿块的细针抽吸活组织的细胞学涂片显示粒系细胞中可见中性中幼粒细胞、中性晚幼粒细胞、中性杆状粒细胞、中性分叶核粒细胞;红系细胞中可见早幼红细胞、中幼红细胞、晚幼红细胞;并可见颗粒巨核细胞、产血小板的巨核细胞、散在血小板及成熟淋巴细胞;细胞形态及各期细胞分布与骨髓片相似,为髓外造血组织。

Apart from the red cell surface, erythropoietin's receptor is also expressed in a large variety of normal tissues.

促红细胞生成素的受体除分布在红血球表面外,也在大批正常组织表达。

Results showed that DCF with different dosages could increase the frequency of micronucleated erythrocytes markedly,except the first injection of the dosage with 5 and 10 mg/kg,every group caused an increase in the frequency of nuclear abnormality erythrocytes,the effects in cell membrane and cytoplasm were less than the effects in karyon by DCF,and the most toxicity was exhibited after the second injection.

结果表明,注射DCF后所有剂量组均能引起中华蟾蜍红细胞微核细胞率显著升高;除5,10mg/kg剂量组在第1次注射后较对照组低外,其他注射剂量和次数均引起核异常细胞率升高,DCF对细胞膜与细胞质的影响小于其对细胞核的影响,在4次注射中以第2次注射后对红细胞的毒性效应最大。

On low-magnification light microscopy, the specimen had a patternless tissue culture-like appearance with increased mitotic actiity, randomly oriented spindle cells, and extraasated red blood cells (Figure 2B ).

低倍光学显微镜下,标本显示无图案的&组织培养样&表现,伴有有丝分裂活性增高、未定性的梭形细胞和红细胞外渗(图2b),组织病理学所见符合结节性筋膜炎。

Figure 2.B, On high-power magnification microscopy, the specimen showed a patternless tissue culture-like appearance with multiple mitoses and extraasated red blood cells.

图2B,高倍显微镜检查,标本显示无图案的&组织培养样&表现,伴有大量有丝分裂和红细胞外渗。(缩略图,点击图片链接看原图

Considerable progress has been made in our understanding of the molecular basis of Rh in the past 10 years. The bases of Rh inheritance, RH gene and its evolution, the structure and function of Rh complex, as well as nonerythroid Rh homolog, have been determined.

过去的10年间,Rh血型的研究取得了极大的进展,得到了大量分子生物学的研究资料,如Rh血型的遗传方式、RH基因及其进化过程、Rh复合体的结构与功能、红细胞外Rh相似蛋白的表达,Rh基因分型技术也进一步得到发展与改进。

Except RDW,MPV and LYM pa-rameters,ADM could induce almost every parameter,espe-cially the RBC,WBC and PLT,to decrease significantly,which was more clear with the increasing of ADM dosage.

ADM可引起外周血除红细胞分布活力、平均血小板体积和淋巴细胞百分比外几乎所有的指标降低,红细胞、白细胞、血小板3种血液成分降低显著(P.05),并随ADM剂量的增加而愈发明显。

Results:The results of these 2 methods were(66±17)×109/L and(98 ±2 0)×109/L,their difference was significant(P<0.001).Their influential f actors were large platelets,platelet clumps,cryoglobulins and schistocytes besid es microcytes.

结果:仪器法结果为(66±17)×109/L,而显微镜计数法为(98±20)×10 9/L,两法结果差异有显著性(P<0.001);URI报警时除小红细胞干扰外,还有其他诸多因素;大血小板、血小板凝集块、冷球蛋白、衰老破坏的红细胞碎片等,其中最常见的为大血小板。

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