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The microheterogeneity and tryptic fragments of glycoprotein recombinant human erythropoietin were analyzed by high performance capillary electrophoresis.

用高效毛细管电泳测定了重组人促红细胞生成素的微多相性、肽图,同时采用毛细管电泳质谱联用技术鉴定了部分非糖基化胰酶消解片段和O-126糖基化肽的结构。

After administration of L-N〓-nitro-Arginine, a specific antagonist of NOS, it was found that the basal and stimulated NO release of blood vessels (aorta, internal carotid, renal and mensenteric artery) was decreased further. The increase of coronary blood flow stimulated by Ach was also reduced further, while the platelet aggregation degree and erythrocyte aggregation index increased significantly. Pathological examination indicated that aortic endothelium was destroyed, the blood vessels in cerebral, cardiac and renal tissues were hypertrophied further and some were almost occluded. The above fin dings resulted in SHR feeded with L-NNA having high incidence of stroke rate and high degree of renal cirrhosis when compared to control SHR. The infarct and hemorrhagic focuci in cerebral tissues, infarct focuci in cardiac tissues, atrophy and hyalinedegeneration of renal glomeruli and degeneration even necrosis of renal tubuli in renal tissues were al so found. The increase ratio of brain and heart to body weight and decrease ratio of kidney to body wei ght further demonstrated that the cerebral, cardiac and renal injuries were aggravated.

在上述结果的基础上进一步给予SHR NOS特异性拮抗剂左旋亚硝基精氨酸后,血管(胸主、颈内、肾及肠系膜动脉)NO基础释放及激动剂刺激释放进一步减少,Ach刺激冠脉流量的增加和胸主动脉内膜NOS活性也进一步减少,血小板聚集程度及红细胞聚集指数显著上升,病理切片显示胸主动脉内皮细胞破坏明显,脑心肾组织内的血管进一步增厚甚至几乎阻塞,导致饲L-NNA的SHR缺血性及出血性脑卒中发生率显著高于对照SHR,脑体重比显著升高;心肌内出现明显的缺血梗塞疤痕灶,心体重比显著升高;肾小球明显萎缩、玻璃样变性,肾小管也变性甚至坏死,使肾硬化程度显著增加呈颗粒性固缩肾,肾体重比也显著减少。

Orientalis in red blood cells from an infected calf was isolated. cDNA was synthesized by reverse transcriptase, amplified by PCR and ligated into λTriplEx2 vector. The recombined vectors were packaged and the unamplified cDNA library was constructed.

用东方巴贝虫感染牛,提纯红细胞内虫体的总RNA,反转录合成cDNA, PCR扩增后将其连接于噬菌体载体(λTriplEx2),通过体外包装,建成东方巴贝虫cDNA文库,并进行扩增。

Undergeneral anesthesia, hearts from New Zealand rabbits were rapidlyexcised and mounted on an erythrocyte-perfused and isolatedheart preparation.

在全身麻醉下,快速分离新西兰兔的心脏,建立红细胞灌注的离体心脏模型。

Objective To investigate the existence of the erythropoiesis inhibitors.Methods The present study included twelve undialyzed ESRD patients with anemia.

目的 本文采用甲基纤维素细胞培养的研究方法,分析尿毒症患者血清对小鼠骨髓红系祖细胞集落形成的影响,初步探讨红细胞生成抑制因子的存在。

Http://www.44dx.com Objective To investigate the existence of the erythropoiesis inhibitors.Methods The present study included twelve undialyzed ESRD patients with anemia.

http://www.44dx.com 目的本文采用甲基纤维素细胞培养的方法,分析尿毒症患者血清对小鼠骨髓红系祖细胞集落形成的影响,初步探讨红细胞生成抑制因子的存在。

In this study, We used longer DD primers in combination with a two-step PCR protocol to overcome these two problems in term of modified methods of Martin and Pardee.K562 erythroleukemia cell differentiation induced by HEMIN or PMA for 36 hours was used as erythriod or megakaryocytic differentiation model in vitro.Total RNAs were extracted from induced and uninduced K562 cells and were applied to mRNA differential display analysis.

为解决此问题,我们参照Martin和Pardee的改进方案[3],建立了一种使用长引物,进行二步PCR的改进DDRT-PCR方法,以氯高铁血红素和肉豆蔻佛波酯诱导K562细胞36小时作为体外红细胞和巨核细胞分化模型,取诱导前后的细胞总RNA进行mRNA差异显示分析。

Methods: Mononuclear cells in umbilical blood and peripheral blood in normal pregnant women and unpregnant women were labled by erythrocyte specific antibody CD71 and fetal specific antibody HbF. Furthermore, NRBC were sorted by flow cytometry.

选用红细胞特异性抗体CD71、胎儿特异性抗体HbF对正常孕妇外周血、未孕妇女和胎儿脐血单个核细胞进行标记,并结合流式细胞仪对孕妇外周血中胎儿有核红细胞进行分选。

Methods C3b sensitized yeast cells and C3b unsensitized yeast cells were used to detect C3b receptors and immune complex on red cells,method of xanthine oxidase was applied to detect SOD activity in red cells.

实验利用低碘饲料和碘化钾去离子水复制了不同碘营养状态的大鼠,采用C3b致敏和未致敏的酵母菌及黄嘌呤氧化酶法分别检测红细胞膜上的C3b受体、免疫复合物及红细胞内SOD的活性。

Results: Serum testosterone levels in uremic male patients with anemia were significantly lower than those in normal controls.

随后40例采用促红细胞生成素进行常规治疗,另40例患者则使用睾酮类药物联用进行治疗,并对各治疗组的结果进行评价。

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