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红细胞

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Methods Using PCE micronuclear test, Chinese hamster lung cell chromosome aberration experiment and rat teratogenicity, to observe the toxicity of free MMC and the MMC absorbed onto ACNP on heredity and reproduction.

采用小鼠骨髓嗜多染红细胞微核试验、中国仓鼠肺细胞染色体畸变试验及致畸试验对受试物ACNP-MMC进行遗传毒性及致畸性检测。

Conclusions】 It is suggested intramuscular injection is an effective immune route. Mice inoculated with coding different stage gene recombinants alone or mixedly could all induce increased humoral and cellular immune response, and NK cell activity.

提示肌肉注射为一有效的DNA疫苗免疫途径,采用编码恶性疟原虫有性期阶段或无性红细胞阶段的重组质粒单独或两者混合免疫小鼠,均能诱导明显的体液免疫反应、细胞免疫反应和NK细胞杀伤活性。

High dose copper induced decrease ofⅠ,Ⅱ,Ⅳ-2 content and increase ofⅢof spectrin. High dose copper can also decrease SA, PE content and increase SM, PS content.These showed that high dose can induce injury of erythrocyte by changing elementary structure and moity of membrane protein and membrane phospholipid, which may be one of the mechanisms of goat erythrocyte damage induced by high dose copper. 2. High copper caused the decrease of SOD and GSH-PX activity and the increase of MDA content.

高铜能引起红细胞膜收缩蛋白Ⅰ、Ⅱ及区带Ⅳ-2蛋白含量均显著降低,而区带Ⅲ蛋白含量明显增高,红细胞膜唾液酸含量降低,磷脂酰乙醇胺含量降低,神经鞘磷脂和磷脂酰丝氨酸含量升高,表明高铜能够改变红细胞膜蛋白和磷脂等基本结构和组成成分,这可能是高铜致羊红细胞损伤的机制之一。

2B8a was weakly reactive to neutrophils (23.72%) and negative for T cells, NK, DC, RBC and Plt. The antibody reacted to all 3 marrow CD34+ cells with an average positive rate of 39.33% while it was negative for G-CSF-mobilized CD34+ peripheral blood stem/progenitor cells (PBSC, 1.25%). Cell line analysis showed that the antibody notably reacted to three out of 4 cell lines (Raji, SMS-SB, Nalm-6 and Nall-1) with the positive rates of 98.78%, 98.61%, 94.93% respectively and weakly to one of them with 5.68% in B lineage cell lines and monoblastic cell line (U937, 67.78%) while it was only weakly positive or negative for other myeloid leukemia cell lines including Meg01 (33.40%), HL-60 (29.70%),K562 (28.19%), KG1a (16.23 %) and HEL92.1.7 (8.02%). Among 4 T lineage leukemia,5 neuroblastoma and 1 colon cancer cell lines tested, only Molt-3 was found weakly positive (31.40%) for 2B8a, while the remaining 3 T cell lines (Molt4, JM and CCRF-CEM), 5 neuroblastoma cell lines (LA-N1, KCNR, BE, SK-N-SH, SK-N-AS) and the colon cancer cell line (HR8348) tested were negative.

结果表明: 2B8a抗原在外周血B细胞上表达(3/3例,平均阳性细胞数为26.29 %),而在T淋巴细胞和NK细胞上不表达(0/3例);在粒细胞和单核细胞上阳性表达均为2/3例,平均阳性细胞数分别是23.72 %和59.84 %;在DC细胞、红细胞和血小板上均不表达(0/3例)。2B8a抗原在骨髓CD34+细胞上的阳性表达是3/3例,平均阳性细胞数39.33 %,而在G-CSF动员的外周血CD34+细胞上的阳性表达仅1/3例,平均阳性细胞数为1.25 %。2B8a抗原在B系细胞系Raji、SMS-SB、Nalm-6和Nall-1上的平均阳性细胞数分别为98.78 %、98.61 %、94.93 %和5.68 %;在T系细胞系Molt-3上的平均阳性细胞数为31.40 %,而在Molt-4、JM和CCRF-CEM 细胞上不表达;在髓系细胞系U937、Meg-01、HL-60、K562、KG1a和HEL92.1.7上的平均阳性细胞数分别为67.78 %、33.40 %、29.70 %、28.19 %、16.23 %和8.02 %;在神经母细胞瘤细胞系SK-N-SH、KCNR、BE、LAN-1和SK-N-AS细胞以及结肠癌细胞系HR8348细胞上均不表达,而在羊膜细胞系FL细胞上呈一定的阳性表达,平均阳性细胞数为45.03%。

Cell line analysis showed that the antibody notably reacted to three out of 4 cell lines (Raji, SMS-SB, Nalm-6 and Nall-1) with the positive rates of 98.78%, 98.61%, 94.93% respectively and weakly to one of them with 5.68% in B lineage cell lines and monoblastic cell line (U937, 67.78%) while it was only weakly positive or negative for other myeloid leukemia cell lines including Meg01 (33.40%), HL-60 (29.70%),K562 (28.19%), KG1a (16.23 %) and HEL92.1.7 (8.02%). Among 4 T lineage leukemia,5 neuroblastoma and 1 colon cancer cell lines tested, only Molt-3 was found weakly positive (31.40%) for 2B8a, while the remaining 3 T cell lines (Molt4, JM and CCRF-CEM), 5 neuroblastoma cell lines (LA-N1, KCNR, BE, SK-N-SH, SK-N-AS) and the colon cancer cell line (HR8348) tested were negative.

结果表明: 2B8a抗原在外周血B细胞上表达(3/3例,平均阳性细胞数为26.29 %),而在T淋巴细胞和NK细胞上不表达(0/3例);在粒细胞和单核细胞上阳性表达均为2/3例,平均阳性细胞数分别是23.72 %和59.84 %;在DC细胞、红细胞和血小板上均不表达(0/3例)。2B8a抗原在骨髓CD34+细胞上的阳性表达是3/3例,平均阳性细胞数39.33 %,而在G-CSF动员的外周血CD34 细胞上的阳性表达仅1/3例,平均阳性细胞数为1.25 %。2B8a抗原在B系细胞系Raji、SMS-SB、Nalm-6和Nall-1上的平均阳性细胞数分别为98.78 %、98.61 %、94.93 %和5.68 %;在T系细胞系Molt-3上的平均阳性细胞数为31.40 %,而在Molt-4、JM和CCRF-CEM 细胞上不表达;在髓系细胞系U937、Meg-01、HL-60、K562、KG1a和HEL92.1.7上的平均阳性细胞数分别为67.78 %、33.40 %、29.70 %、28.19 %、16.23 %和8.02 %;在神经母细胞瘤细胞系SK-N-SH、KCNR、BE、LAN-1和SK-N-AS细胞以及结肠癌细胞系HR8348细胞上均不表达,而在羊膜细胞系FL细胞上呈一定的阳性表达,平均阳性细胞数为45.03%。

Methods The Kunming mouses were inoculated with blood from the Mycoplasm suis infected pigs, the rate of infection and histopathological changes were determined in different stages after challenge.

用感染了附红细胞体的猪血液感染昆明小鼠,定期进行感染率和病理组织学检测。

Extracellular particles, small mononuclear cells, red blood cells, and myoepithelial cells were markedly decreased in number.

胞外成分,小单核细胞,红细胞,肌上皮细胞数量上显著减少。

Methods: Forty mice were divided into four groups randomly:normal group,model group,ligation group and treatment group.The model was made by the method of partly ligating the recta outside the body.The level of endotoxin in blood plasma was measured by dynamic nephelometric method and the content of TNF-a in blood serum was detected by the method of radioimmunity.(2)After the mice were killed,their left lungs were taken out for bronchi-alveoli fluid, then the content of MDA in BALF was tested by TBA method.(3)The vitality of PAM in BALF was countied on the counting set of erythrocyte,and the blue-dyed cellswere counted, then the percentage of PMN was worked out.(4)The pathological features of the mice\'s lung tissue were observed with routine and ultramicro pathological mechanism.

①将40只大鼠随机等分为正常组、模型组、解扎组、治疗组,以体外直肠不全结扎法造模,以动态浊度法检测各组大鼠血浆内毒素水平,放射免疫分析法检测各组血清TNF-α含量;②大鼠处死后,取左肺进行支气管肺泡灌洗,以TBA法测定支气管肺泡灌洗液中丙二醛含量、白蛋白含量及肺表面活性物质水平;③在红细胞计数盘上作BALF中肺泡巨噬细胞活力计数,计数兰染的细胞数,求出死亡百分率;在光镜下计数BALF沉渣200个细胞,计算中性粒细胞所占百分比。

Objective: To evaluate the effect of Lcarnitine plus rhEPO on nephritic anemia.

目的:观察左旋卡尼汀联合重组人促红细胞生成素治疗肾性贫血的临床疗效。

Methods: Urinary specimens from nephros 37 patients were analyzed by UF-100 and reports from microscopic examination of urinary sediments were compared with the quantity of urinary RBC and WBC.

选取肾内科门诊病例15例和住院病例22例,分别用尿液沉渣标准化检查所推荐的方法与UF-100流式尿沉渣全自动分析仪分析尿液中红细胞、白细胞的含量。

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