繁殖体

The results showed that : 1 During nonbreeding season, the giant panda′s ovary was still rich with large and small follicular oocytes, and the large follicular oocytes could be matured in vitro; 2 The mean diameter of the oocytes was (141±6.7)μm and lots of cortical granules contained in the cytoplasm of oo...

结果表明∶（1）在非繁殖季节，大熊猫卵巢中小腔及大腔卵泡卵母细胞仍较丰富，其大腔卵泡卵母细胞可在体外培养成熟；（2）大熊猫的卵母细胞平均直径为（141±6.7）μm ，其胞质富含卵黄颗粒；（3）自配 PandaI I号获能液（含丙酮酸钠、肾上腺素等）可使大熊猫精子有效获能；（4）大熊猫卵母细胞体外受精后，经培养可观察到第2 极体，有的受精卵开始发生卵裂。

20 Centuries 60 time, american Gristina put forward, all biology material are embedded inside body hind, its surface all can form a kind of biomembrane, bacterial criterion is many in this kind of biomembrane progenitive, the many mucilage outside producing a cell is qualitative (Extracellular Slime Substance, ESS), it is the easy hair sex that causes false body infection and the main factor that study a gender hard.

概要： 20世纪60年代，美国Gristina等提出，所有的生物材料植入体内后，其表面均能形成一种生物膜，细菌则在这种生物膜中大量繁殖，并产生细胞外多粘质物质(Extracellular Slime Substance，ESS)，是导致假体感染的易发性和难治性的重要因素。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

中文题名斜带石斑鱼生长激素分泌及其基因表达调控的研究副题名外文题名 Regulation of growth hormone and its gene expression in orange-spotted grouper，Epinephelus coioides 论文作者冉雪琴导师林浩然学科专业水生生物学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2003 论文页码总数140页关键词生长激素基因表达石斑鱼馆藏号BSLW /2003 /Q959 /39 应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

Psittaci can survive and multiply in the free-living amebae, suggesting the possibility that Acanthamebae may be an additional host for C. psittaci.

结论鹦鹉热衣原体能在棘阿米巴内生存和繁殖，环境中自由生活阿米巴可能成为鹦鹉热衣原体的贮存宿主。

Quadratic regressive factorial experiment was used to optimize concentration of 6-BA and NAA in MS medium for shoot propagation from the top buds and stem segments of Russelia equisetiformis.

以爆仗竹顶芽与带腋芽的茎段为外植体，采用二次回归正交设计方法研究了爆仗竹组织培养快速繁殖中不定芽增殖系数与继代培养基中6BA和NAA组合及其浓度配比之间的关系。

Results The morphology and asexual reproduction of Cloudina are broadly similar to modern serpulid annelids and its flourishing is somewhat coeval and corresponding to a positive excursion of δ13C.

结果 Cloudina可能是多毛类龙介虫，二者在管体形态特征、无性繁殖方式等方面存在可比性；Cloudina的出现都对应着碳同位素的明显正漂移，具有基本等时的特点。

The reproductive area of certain algal thalli, e.g.

2孢子囊：某些海藻藻体的繁殖区域。

Not long time later, due to the continuous migration and not-stop reproduction, the microbial thalli were distributed widely in the confine between the initial places and the oil-water interface.

可视化实验表明，由于不断的运移和不停的繁殖，在微生物初始位置与油水界面之间的范围内，广泛地分布着微生物菌体。

The mating system and breeding ecology of Cabot's tragopan were studied through radiotelemetry and field observation.

1990年11月至1993年6月，在浙江省乌岩岭自然保护区通过对11只成体的无线电遥测以及野外观察，对黄腹角雉的繁殖生态学进行了研究，所得结果如下

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。