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Study on Tissue Culture and Rapid Propagation of Oxalis triangularis

以三角紫叶酢浆草丛生芽和叶片为外植体,研究不同激素水平对三角紫叶酢浆草组织培养的影响,探讨三角紫叶酢浆草快速繁殖的最佳途径。

However, reproduction is often by parthenogenesis since polyploid animals are often sterile.

然而,它们常常是通过孤雌生殖繁殖的,因为多倍体动物往往不育。

In the adult, or medusa stage of a jellyfish, they can reproduce sexually by releasing sperm and eggs into the water, forming a planula.

在海蜇成体或水母体阶段,它们通过将精子和卵于释放到水中形成浮浪幼体而进行有性繁殖。

For expanding propagation coefficient and overcoming influence between genotype in the course of cultivation, Stem of Niujiao pepper asepsis seeding was cultivated in this paper, and the fitting stem bud and rootage substrate were screened out.

为了进一步扩大繁殖系数,克服其他外植体培养过程中基因型的影响,利用牛角椒的无菌种苗进行了辣椒茎段培养,筛选出了适宜的诱导茎段萌芽培养基和生根培养基。

The results showed that the frequency of germination were able to be increased by keeping seed vessel, culturing seeds with radicle up and pre-treating seeds in 5.0mg/L GA3 solution for 12h. The frequency of explant-browning was reduced by adding AC 0.5mg/L to induction media and the vigor of plantlets was promoted by the AC-adding. The propagating coefficient could be improved and the frequency of bud-vitrifration increased by adding 150mg/L coconut water.

结果表明发芽时保留种皮、接种时胚根向上对蚕豆种子的萌发有着至关重要的影响,用5.0mg/L GA3溶液浸种12h有利于种子萌发;培养基中添加0.5g/L AC能有效抑制外植体的褐化,并能使植株生长健壮;培养基中添加150mg/L椰汁能提高繁殖系数,有效抑制玻璃苗的发生。

Pollen is the male_gamete of seed plant, which plays an important role in sexual reproduction.

花粉是种子植物的雄配子体,在有性繁殖中发挥着重要作用。

Microorganism in bioreactor had well growth, and could form stable active zoogloea.

生物反应器中微生物繁殖良好,能够形成稳定的活性菌胶团絮体。

Some species of sturgeons are polypolids. The hybridization is common even between genera Acipenser and Huso. The parents of different species with the same chromosome numbers will produce reproductive offsprings, and thus, genetic introgression among species would easily happen in the nature conditions.

鲟具有多倍体特徵,其中鲟属及鳇属杂交的情形非常普遍,染色体倍数相同的亲代杂交产生的子代具有繁殖力,因此在自然情况下或人为移殖时很容易造成基因渗入现象。

For the bud directly differentiation , the suitable culture medium was MS+BA 2mg/L +NAA 0.2mg/L or MS+TDZ0.01mg/L +NAA0.2 mg/L, of which the induction rate was 88% and 100% respectively. An efficient tissue culture system has been developed with the bud of mature seed of Aesculus hippocastanum L. as explants . Buds were induced from 2cm high young plantlet cultured on MS medium supplemented with 0.6 mg/L 6-BA plus 0.1mg/L or 0.4~0.6mg/L ZT plus 0.1 mg/L NAA for 15 d, and the induction rate was 100%, the mean No. of buds was 35.7; The combination of MS+0.2mg/L 6-BA +0.1mg/L NAA + 10mg/L AD was the suitable culture medium for elongation of the buds.

以欧洲七叶树成熟种子的胚芽进行离体培养和快速繁殖,结果表明:高约2cm的无菌苗在MS+0.6mg/L 6-BA+0.1 mg/L NAA或MS+0.4~0.6mg/L ZT+0.1 mg/L NAA培养基上培养15 d左右可诱导出不定芽,分化频率为100%,平均每株产芽35.7个;MS+0.2mg/L 6-BA+0.1mg/L NAA+10mg/L AD培养基有利于芽伸长;生根培养基为1/2MS+0.4 mg/L NAA+0.2mg/L IBA时,生根率可达75%。

Taking leaf and hypocotyl of Limonium bicolor as the explant, the system of rapid propagation were established, the influences of hormone concentration to induce the tissue culture and adventitious buds were studied.

方法]以二色补血草的子叶与胚轴作为外植体,建立快速繁殖体系,研究不同激素浓度对诱导愈伤组织、不定芽以及生根率的影响。

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