紫光

Http://www.44dx.com objectiveto investigate the therapeutic effects of subconjunctival verapamil on outcome in an experimental model of traumatic proliferative vitreoretinopathy.methodsan experimental model of traumatic proliferative vitreoretinopathy was induced in pigment rabbits,which then were selected randomly to receive either subconjunctival verapamil injection treatment or a placebo injectiondaily for 3 weeks.animals were examined by indirect ophthalmoscopy at weekly intervals for 5 weeks.eyes were enucleated for light microscopy 5 weeks later.resultsfifty-six percent(18 of 32)of the rabbits receiving subconjunctival verapamil injection had developed tractional retinal detachment,whereas eighty-one percent(26 of 32)of control animals had developed tractional retinal detachment(χ2=4.655,p=0.031).the results of clinical examination and light microscopy didn?

http：//www.44dx.com 目的观察维拉帕米(verapamil，ver)对眼后节穿通伤增生性玻璃体视网膜病变(proliferative vitreoretinopathy，pvr)的影响。方法64只青紫蓝兔，每只兔任选其中一只眼制备外伤性pvr模型后随机分为两组，治疗组在模型制备后立即每日结膜下注射2.5 mg/ml的ver0.5ml，对照组则每日结膜下注射生理盐水0.5ml，共21天。模型制备后7天内每天用裂隙灯显微镜及间接立体检眼镜观察伤口、角膜、前房及眼底情况，此后每周第3，7天各检查1次。伤后第35天时常规摘除模型制备眼作光镜观察。

Methods Anhydrous acetic acid as solvent, 0.1 mol/L perchloric acid as titrant, crystal violet solution as indicator to establish non-aqueous titrations and ODS column as stationary phase, methanol and a mixture of 0.025 mol/L sodium phosphate monobasic and 0.02 mol/L sodium dedecyl sulfate (80:20) adjusted with phosphoric acid to a pH (4.8±0.1J as mobile phase, 220 nm as detective wavelength to establish HPLC-UV method for determination of piperazinylethylestrone drug substance; FMOC-CL as a derivatization reagent, FD as a detector to establish an HPLC method with derivatization and column switching for determination of piperazinylethylestrone in dog plasma.

分别采用无水冰醋酸为溶剂，0.1mol/L高氯酸为滴定液，结晶紫指示液指示终点的非水滴定法和HPLC-UV法测定哌嗪雌酚酮原料药含量；以FMOC-CL为衍生化试剂，采用柱切换柱前衍生化萤光检测的HPLC法测定狗血浆中哌嗪雌酚酮含量。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

"The earliest Egyptian bead s (c. 4000 BC) were made of stone, feldspar, lapis lazuli, carnelian, turquoise, hematite, or amethyst and were variously shaped (sphere, cone, shell, animal head)."

最古的埃及珠饰年代约为公元前4000年，一般为石制(绿长石、杂青金石、光玉髓、绿松石、赤铁矿和紫晶)，形状有球形、圆柱形、贝壳形或动物头形。

"The earliest Egyptian beads (c. 4000 BC) were made of stone, feldspar, lapis lazuli, carnelian, turquoise, hematite, or amethyst and were variously shaped (sphere, cone, shell, animal head)."

最古的埃及珠饰年代约为公元前4000年，一般为石制(绿长石、杂青金石、光玉髓、绿松石、赤铁矿和紫晶)，形状有球形、圆柱形、贝壳形或动物头形。

The properties of light-adapted and dark-adapted BR D96N films and their transformation mechanism were studied by measuring and analyzing the kinetic absorption spectra of the sample under light illumination.

通过对菌紫质D96N基因突变形成的薄膜样品在光照下动态光谱的测定和分析，研究了此样品的光适应型、暗适应型特性及其之间的转化机制。

Luminous add-ons for these rings include amethysts, sapphires, diamonds, rubies, and pearls.

夜光附加这些戒指包括紫水晶，蓝宝石，钻石，红宝石和珍珠。

Fashioned in polished 925 sterling silver, it boasts a beautiful open petal design that's light and airy, with five purple amethysts accenting the center.

925纯银抛光过时，它拥有一个美丽开放的花瓣设计与重音中心5紫色的紫水晶的光线和通风。

Using KL-1 photocatalytic reactor with 20 w UV light as light source,particle size in average a parth for nano TiO_2 suspension system of 30 nm,Methyl Violet 5BN can be efficiently decoloured,and there is the maximum decolorization rate of Methyl Violet 5BN in strong acid solution.

以脱色率为主要考察指标，研究了纳米TiO2光催化氧化法处理三苯甲烷染料碱性紫5BN的最佳工艺条件。

Cattle eggs red: also known as "fimbristipula Hance's" leaf-shaped, 145,290, Hi negative plants growing in the backlight, overcast mountain crevice temperature in each year, picking the best time for the Dragon Boat Festival, its flavor with acid, which is rich in amino acids, can be used for tea kitchen, add sugar, tart moderate , is pure natural drinks, radicals can lower blood pressure, the rule bonesetter pain, bleeding, and so scattered, if chicken, ribs food, but also delicious and pleasant.

牛卵红：又名&紫背天葵&，叶片状，呈暗红色，喜阴性植物，生长于背光、阴温的高山石缝中，每年端午节为最佳采摘时期，其味带酸，含有丰富的氨基酸，可作茶泡饮，加糖，酸甜适中，是纯天然饮品，常饮可降血压，治跌打疼痛，散瘀血等，若炖鸡、排骨食用，更是味香怡人。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。