素连接

The result showed that 5-fluorouracil could link to the peanut agglutinin by EDC/NHS crosslinking with high drug ratio.

结果表明碳二亚胺法可成功实现药物与花生凝集素的连接，该法制备得到的结合物其药物结合率高。

Calicheamicin can be conjugated to the non-specific antibody using a 4-(4'-acetylphenoxy) butanoic acid or (3-Acetylphenyl) acetic acid linker.

可使用4-(4′-乙酰基苯氧基)丁酸或(3-乙酰基苯基)乙酸连接子使卡奇霉素与所述非特异性抗体共轭结合。

Conclusions— Junctin is an essential regulator of sarcoplasmic reticulum Ca release and contractility in normal hearts.

结论-连接素是正常心脏肌浆网钙释放和收缩力的重要调节因素。

In order to study the expression and functional characteristics of dehydrin gene during different growth, and make the polycolonal antibody, using wheat plumelet under the water deficit condition as experiment material, and its total RNA was extracted. The target dehydrin gene through RT-PCR was got, connect to the cloning vector PUCM-T, recombination expression plasmid PET-32a-wzy1-1 was constructed according to recombination of cloned vector PUCM-T-WZY1-1 of wheat dehydrin gene in this experiment.

为研究小麦在不同时期脱水素基因的表达情况和生物学功能及抗体制备，以小麦幼芽为材料，经干旱胁迫处理后，提取总RNA，通过RT-PCR得到小麦类脱水素基因片段(WZY1-1)，再连接至克隆载体PUCM-T，并成功构建重组表达质粒PET-32a-wzy1-1，将阳性重组质粒转化于受体菌BL21(DE3)感受态细胞中，经IPTG诱导表达，进行表达产物的聚丙烯酰胺凝胶电泳检测。

Transferred the NP-1 gene into C. ellipsoidea by electroporation with high efficiency expression vector pBinUΩNP1. PCR, Southern blot and Northern dot blot analysis of the G418resistant alga strains indicated the stable integration and correct transcription of NP-1 in C. ellipsoidea genome. In vitro antagonistic assays showed the proper expression of defense gene in C. ellipsoidea. Transgenic C. ellipsoidea is antagonistic to gram negative bacteria—Bacillus subtonic, gram positive bacteria—Escherichiacoli and fungi—Fusarium oxysporum to some extent.

以椭圆小球藻完整细胞为受体，采用电激转化法将连接在高效表达载体pBinUΩNP1上的兔防御素NP-1基因导入小球藻，通过PCR、Southern杂交、Northern点杂交对转基因小球藻进行了分子检测，同时通过体外离体抑菌实验检测了兔防御素NP-1基因表达产物的活性，结果表明NP-1基因已稳定整合到小球藻基因组中，并进行了正确转录和表达。

The location and structures of sex-pheromone-producing gland in female H.insularis were studied by EAG,GC,SEM,and TEM.These studies showed that thegland situate in the intersegmental membrane between the eighth and ninthabdominal segments,and is an eversible abdominal fold;Many plump cones disturbon the surface of the gland.The glandular cells of 2-day old virgin female H.insularis are arranged in one layer,among which the central cells are columnarepithelial cells and flat on two sides.The nucleus is irregular elliptical.There isevident conjugation between cells and the involution is more in the basal membraneof cell.Microvilli are distributed on the cytoplasmic membrane and linked withendocuticle on which there are many layers of chitin,and the outer cuticule is staineddeeper.The cell contains bubbles,mitochondria,glycogen deposits,roughendoplasmic reticulum and smooth endoplasmic reticulum.

结合触角电位、毛细管气相色谱、扫描电镜、透视电镜等技术对小线角木蠹蛾雌蛾腹尖末端不同组织部位提取物的测定分析以及腺体位置和形态结构的观察发现：小线角木蠹蛾性信息素分泌腺位于腹部末端8～9节之间，是一个由节间膜特化而成的上皮结构，为一可外翻的腹褶，腺体表面分布着饱满的锥形体，羽化后2天未交尾的雌蛾腺体细胞呈单层排列，腹面中央由密集的柱形上皮细胞组成，细胞排列向两侧延伸至背部，其形状由柱形逐渐变为扁平形，细胞核为椭圆形，细胞与细胞间有明显的胞连接，细胞基底膜基褶较多，质膜上分布着微绒毛，并与内表皮连接，内表皮上有多层几丁质，外角质层染色较深，细胞质中含有空泡、线粒体、脂质粒、粗面内质网和光面内质网。

But in day 14th and day 28th groups, the ratio of macrophagus positive area in lung interstitium was bigger than that of control group（ P 〈0.05 ）, with collagen fibers depositing in the lung interstitium gradually.

结论博莱霉素鼠肺毛细血管内皮细胞间紧密连接持续开放，与巨噬细胞在组织间浸润密切相关，并可能是博莱霉素致肺纤维化发生的重要因素。

During our strategy, two linear amplification approaches are employed, ligase detection reaction and an isothermal amplification (cooperation by DNA polymerase and nicking enzyme).

连接产物由于在DNA聚合酶和限制性内切酶的共同作用下，发生等温扩增反应，扩增出大量的血红素的适配子，适配子与血红素结合，形成的复合物催化鲁米诺和双氧水的化学反应。

Total synthesis of bilobetin and isoginkgetin, natural polyhydroxy biflavones with a Ⅰ-3'/Ⅱ-8 dissymetrical connection, and synthesis of acetyl flavones were investigated in this dissertation.

本论文对天然多羟基不对称连接的双黄酮-白果素、异银杏素的合成以及乙酰基黄酮的合成进行了研究，共包括以下内容

With this set of criteria, the literatures published 3—O—L—rhamnosyl—(1→2/1→4)—L— rhamnoside, 3—O -D—xylosyl—(1→2/1→4)-L-rhamnoside and 3-O-D — glucosyl-(1 →2/1→4)—L— rhamnoside structures of anhydroicaritin and desmethylanhydroicaritin were re-evaluated.

本文以上述双糖1→2连接的光谱标准，对文献报道的脱水淫羊藿素和去甲脱水淫羊藿素3—O—L一鼠李糖—(1→2或1→4)—L—鼠李糖甙、3—O—D—木糖—(1→2或1→4)—L—鼠李糖甙及3—O—D—葡萄糖—(1→2或1→4)—L—鼠李糖甙类化合物的结构进行了重新评价。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。