素因子

Pheromone traps; sex pheromone; anemotaxis; lures; disparlure; integrated control; attractants; chemical senses; chemoreception; chemoreceptors; infochemicals; semiochemicals; chemical stimuli; chemical communication; chemical cues; chemical perception; pheromones; pheromone receptors; pheromone biosynthesis-activating neuropeptide; pheromone biosynthesis; pheromone-binding protein; PBAN

信息素诱骗；性信息素趋风性；诱惑；舞毒蛾性引诱剂；综合防治；引诱剂；化学知觉；化学感受；化学接受体；信息化学；化学信息素；化学刺激物；化学通讯；化学因子；化学感觉；信息素；信息素受体；信息素生物合成-激活神经肽；信息素生物合成；信息素-结合蛋白；PBAN

TGF-β s are multifunctional cytokines with a broad range of biological activities. TGF-β s belong to a family of related proteins, which demonstrate various degrees of amino acid sequence homology; this protein family is called the TGF-β superfamily. Other members of this family include the bone morphogenetic proteins, inhibin, activin and Mullerian substance.

TGF-β类生长因子是一类具有广泛生物学活性和重要临床应用价值的细胞因子，包括多种蛋白因子，它们在氨基酸结构上具有不同程度的同源性，共同构成了TGF-β超家族，包括BMPs、活化素、抑制素和缪勒氏物质等。

METHODS: Female BALB/c mice were treated with CCK-8 and keyhole limpet haemocyanin, splenocytes were acquired and incubated in vitro with KLH restimulation, the productions of Th1 cytokines, interferon-γ, interleukin-2 and Th2 cytokines, IL-4, IL-5 in cell culture supernatants were detected by enzyme-linked immunosorbnent assay. The mRNA expressions of IFN-γ, IL-2, IL-4 and IL-5 in splenocytes were detected by reverse transcription-polymerase chain reaction. The levels of Th1 antibody IgG2a and Th2 antibodies IgG1 in serum were also detected by ELISA.

给予BALB/c小鼠钥孔戚血蓝蛋白免疫同时体内给予不同剂量的CCK-8，酶联免疫吸附试验检测其脾细胞培养上清中Th1型细胞因子γ-干扰素、白细胞介素-2（IL-2）和Th2型细胞因子白细胞介素-4（IL-4）、白细胞介素-5（IL-5）水平，逆转录聚合酶链式反应法检测脾细胞中IFN-γ、IL-2、IL-4、IL-5 mRNA表达；ELISA法检测血清中Th1型抗KLH抗体IgG2a和Th2型抗KLH抗体IgG1水平。

The identification of protein were performed by searching the mass spectrometry data from the NCBInr database using the software of Sequest Bioworks3. 2. The results indicated that over 25 protein components including acid and basis phospholipase A2, nerve growth factor, presynaptic neurotoxin, hemorrhagic factor, thrombin-like enzyme, serine protease, glycogen synthase, metalloproteinase, maturase, L-amino acid oxidase, plasminogen activator, adhesion, cysteine-rich secretory protein, disintegrin, fibronectin-binding protein, fibrinogen clotting inhibitor A chain, vascular apoptosis-inducing protein 1, pyranose 2-oxidase, carbamoyl-phosphate synthase, as well as the homology proteins of snake venom ablomin, ablomin, catrin, mucrofirase, pallabin, salmosin and triflin homologues were identified from the GSSV.

从GSSV中鉴定出超过25种蛋白组分，主要包括：酸性磷脂酶A1、碱性磷脂酶A2、神经生长因子、突触前神经毒素、出血因子、类凝血酶、丝氨酸蛋白酶、糖原合酶、金属蛋白酶、成熟酶、L-氨基酸氧化酶、纤溶酶源激活物、外源凝集素、富含半胱氨酸分泌酶、去整合素、纤连蛋白结合蛋白、纤维蛋白原凝固抑制因子A、血管凋亡诱导蛋白1、氨甲酰基磷酸合成酶、吡喃糖－2-氧化酶以及ablomin， catrin， mucrofirase， pallabin， salmosin和triflin的同源蛋白。

These results indiacated that tranferring macrophages combinedwith cytokines could be a new adoptive immunotherapy protocol foradvanced cancer,and rTNF used in vivo locally could induce andactivate macrophages to kill tumor cells.Monocytes when activatedunderwent a series of phenotypic and functional changes includingthe expression of IL-2R which may provide an important immunoregu-latory pathway.The presence of lectin-like molecules on thesurface of monocytes and tumor cells may bring theeffector/target cells together,thus facilitating the inductionof apoptosis in target cells by triggering the production ofcytolytic factors and the modification of target of targer cell surface anti-gens.(such as HLA-DR).

综合以上结果可以得出以下结论：转输巨噬细胞并结合细胞因子的联合应用是肿瘤继承性免疫中的又一新的、有潜力的方法；抗肿瘤细胞因子rTNF可通过激活巨噬细胞在体内抑制对其不敏感的肿瘤细胞的生长；人单核细胞在一定条件下激活后，可表达IL-2R，进而一方面增强其自身对IL-2的敏感性，另一方面在体内也具有调节T细胞功能的作用；单核细胞和肿瘤细胞上存在的凝集素类受体可促使两种细胞之间在凝集素介导下相互接近，并诱发单核细胞产生细胞毒因子，以及通过调节靶细胞上表面抗原的表达，促进诱导靶细胞的细胞凋亡。

Though the primary stimulator of 1H is not clear, it is related to physical, cell and body fluid IH is a chronic process involving multiple faction injury and blood flowing abnormal are revamped as the main reason, the proliferation level depend on the length and width of the injure of vessel large amount research illustrate injury cause many growth factor infolded in IH, for example: template derived growth factor, fibroblast growth factor, transmission growth factor , endothelin (ET-1) et al, some early responsive gene such as c-fos、c-jun c-myc also take part in SMC proliferation after vessel injury.

虽然IH的确切起始刺激因子并未阐明，但已知与物理、细胞和体液因子有关。IH是一个多因素参与的慢性过程，损伤和血液动力学异常一真被认为是IH的主要因素。内膜增殖的程度有赖于血管损伤的长度和深度。近年来大量的实验研究结果表明，损伤等刺激引起的众多的生长因子样物质在IH中起关键作用。如血小板源性生长因子、碱性纤维母细胞生长因子、转化生长因子β、内皮素-1（ET-1）等诸如C-fos、C-jun、C-myc等早期应答基因也参与了血管损伤后SMC的增殖。

MethodsBone resorption test was carried out using neonatal mouse calvaria to investigate the bone resorbing activity of cytokines (IL 1, IL 6, TNF, GM CSF, and IFN γ).

利用新生鼠头顶骨进行体外骨吸收实验，分别验证细胞因子白细胞介素1（IL 1）、白细胞介素6（IL 6）、肿瘤坏死因子、IFN γ、粒巨噬细胞生长因子在体外对骨吸收的影响。

Methods Bone resorption teat was carried out using neonatal mouse calvaria to investigate the bone resorbing activity of cytokines (IL-1, IL-6, TNF, GM-CSF, sod IFN-γ).

利用新生鼠头顶骨进行体外骨吸收实验，分别验证细胞因子白细胞介素1、白细胞介素6(IL-6)、肿瘤坏死因子、IFN-γ、粒－巨噬细胞生长因子在体外对骨吸收的影响。

RESULTS: Wound healing rate, wound healing time, histopathology analysis, quantity assay of macrophage, determination of hydroxyproline, proliferation of cell, assay of DNA contents and circle of cells, level of transforming growth factor-alpha, levels of interleukin-1, interleukin-6 and tumor necrosis factor, assay of keratinocyte collagenase-1, level of fibroblast growth factor receptor-1, level of monocyte chemoattractant protein-1 and level of keratinocyte plasminogen activator inhabitor type 2 were selected as the evaluation criteria of wound healing.

结果 筛选了创面愈合率、创面愈合时间、组织病理学分析、巨噬细胞定量分析、羟脯氨酸含量测定、细胞增殖情况、细胞DNA含量和细胞周期分析、转化生长因子-α水平、白细胞介素-1、白细胞介素-6和肿瘤坏死因子水平、角质细胞胶原酶-1含量测定、成纤维细胞生长因子受体-1水平、单核细胞化学诱导蛋白-1水平和角质细胞纤溶酶原活化抑制剂-2水平等十三种创面愈合评价指标。

objective the aim of this study is to investigate the expression and the distribution of the nerve growth factor during the period of neural tube development of human embryo.method early development of neural tube was studied in human embryos about 35 gestational days by using immunocytochemical abc technique.result there were ngf immuno-positive substances in the cytoplasm and nuclei of neuroepithelial cells in the ventricular zones of neural tube.in the intermediate zone of neural tube,ngf immunoreactivity was detected in the nuclei of some neurons,or the processes of other neurons which contained no ngf-immunoreactive substances in their nuclei;the expression pattern of ngf in the marginal zone of neural tube was similar to that of the intermediate zone.the density of ngf-immunorecative particles was higher on the rostrum side of neural tube than on the caudal side.the ngf immuno-positive cells were also observed among the somites of embryo under the neural tube.conclusion these results suggest that ngf was an important signal molecule to induce neural tube differentiation,and that ngf may play a significant role in regulation of the biological function of neurons in developing neural tube.

目的 研究捷安肽素的抗真菌作用机理。方法采用形态学方法和同位素标记法。显微形态观察经捷安肽素处理后的供试真菌的形态学变化。进一步采用14c同位素标记的特异底物&尿苷二磷酸-（14c）-葡萄糖&示踪，研究捷安肽素对真菌(1，3)-β-d-葡聚糖合成酶活性反应的影响。结果研究神经生长因子在早期人胚神经管发育过程中的定位表达。方法采用免疫细胞化学 abc法染色，研究35天人胚的发育情况。结果在人胚神经管的室管带中，神经元的细胞质和细胞核ngf免疫反应阳性；在中间带，一部分神经元的细胞核ngf免疫反应阳性，另外一部分神经元的细胞核ngf免疫反应阴性，而其突起ngf免疫反应阳性；在边缘带ngf的表达与中间带相似。在神经管的头侧ngf阳性反应较强，神经管的尾侧ngf阳性反应较弱。结论 ngf在人胚神经管免疫反应阳性，表明ngf可能是诱导神经管分化发育的重要信号分子，提示ngf可能在人胚神经管的发育中具有十分重要的作用。神经生长因子；人胚；神经管；发育

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。