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PCR-SSCP was used to screen exons in 45 Wilson disease patients from 39 Chinese families and in 10 normal controls. Nucleotide sequence analysis by autoradiograph and restriction enzyme digest were used to detect the mutant points.

对中国人WD39个家系45例患者的3~20号外显子进行聚合酶链反应-单链构象多态性分析,对有异常者进行序列分析,并通过对该突变点的酶切再次筛选。

These include that the dealkylation of the aromatics with side chains, polynuclear aromatic hydrogenation under supercritical condition, cracking of naphthene and C-S C-N key.

渣油经过加氢裂化反应后,虽然其芳炭率提高了,但是它的芳香环系缩合度降低了,表明在加氢中有大量的烷基侧链断链反应发生。

Objectives To search for mutations in NOTCH3 gene in four Chinese families with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy.

方法对 4个经临床和病理检查证实的CADASIL家系中的先证者作NOTCH3基因编码区外显子 1~ 12的聚合酶链反应和DNA测序,对家系 2和 4中的部分亲属也作了同样的检查。

The mechanism in which trehalose is produced from dextrin or starch hydrolyzate by endocellular enzymes of bacterium D-97 can be elucidated by high performance liquid chromatography with differential refraction detection basically, including the effect of the different carbon sources on the endocellular trehalose-producing enzymes in bacterium D-97 and the possibility or ability of the endocellular enzymes to produce trehalose using maltooligosaccharides of different chain lengths.

通过高效液相色谱/示差折光检测系统分析可获得细菌D-97利用糊精或淀粉水解物合成海藻糖的基本生物学信息,包括微生物培养碳源对细菌D-97胞内海藻糖合成酶系的影响以及该酶系利用不同种类或不同分子链长度的麦芽寡糖合成海藻糖的能力及作用过程。

Methods Using polymerase chain reaction-single strand conformation polymorphism, all the 41 exons of TSC2 gene were analyzed in 4 TSC cases (include 1 suspect case) from one family and 1 sporadic TSC case, and compared with the kin familial controls and kinless normal controls.

采用聚合酶链反应-单链构象多态性技术,对TSC一家系4例TSC患者(其中1例疑似)、1例散发性TSC患者外周血TSC2的41个外显子进行检测,并与家系中健康对照组和无血缘关系健康对照组进行比较。

Several main non-ionic surfactants (AEO、TX、SpanTweem、EL) was used to emulsify liquid paraffin and di octyl sebacate and the anti-coalescence stability of the corresponding emulsion was studied, the impact of the presence of benzene and double bond in lyophobic moiety of surfactant and its length and matching with dispersed phase upon the interfacial viscosity and Gibbs elasticity was analyzed.

本文采用了几种主要的非离子活性剂(AEO、TX、Span-Tween、EL)来乳化白油和癸二酸二辛酯,通过对各乳液体系抗聚并稳定性的考察,分析了活性剂亲油链段的长度、苯环和双键的存在、及亲油链段与被乳化对象分子结构的匹配关系对界面粘度和Gibbs弹性的影响。

It is composed of a toxophoric A-chain and a galactose-binding B-chain.

箆蔴子毒蛋白质系由一具酵素活性的A-链及一能与半乳醣结合的B-链所组成。

First, N'-(4-aminophenyl)-N'-4-(5-phenyl-1,3,4-oxadiazol-2-ylphenylbenzene-1,4-diamine was prepared and polymerization was carried out by direct polycondensation with a series of dianhydrides to obtain polyimides containig triphenylamine units in the main chain and 1,3,4-oxadiazole moieties as pendent group.

首先合成新型双胺N'-(4-aminophenyl)-N'-4-(5-phenyl-1,3,4-oxadiazol-2-ylphenylbenzene-1,4-diamine后,与一系的芳香族二酐进聚缩合反应,可制得一系列三苯胺为主链且1,3,4-恶二唑为侧链的聚醯亚胺。

Methods A CMT2L proband with 423 mutation in HSP22 gene had been studied and reported by the present authors.

方法应用聚合酶链反应和DNA直接测序方法,对1个发现HSP22基因423突变的CMT2L家系外的114个CMT家系先证者进行了HSP22基因的突变分析。

A wheat- Th.intermedium addition line,which was yellow rust resistance,was found to be homoeologous to group 7 based on endopeptidase isozyme analyses.

对小麦—中间偃麦草部分双二倍体无芒中 4、异附加系 C0 76、宛 71 0 7和中国春进行了肽链内切酶(EP- 1 )等电聚焦电泳,结果表明,肽链内切酶在阳极处有一特异带。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?