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The phyolgenetic trees of Araneoidea were reconstructed with NJ, MP and ML methods.

用NJ法、MP法、以及ML法重建了分子系统树

The results showed that the microbial community could degrade 39.6% of rice straw gross weight within five days. When the culture medium volume was 1/5 of the whole and pH at 6 on the 5 days culture, the CMC reached the highest of 14 IUml^(-1). During the rice straw degradation, more than ten kinds of products were detected using GCMS. DGGE detected the dynamic change of microbial community composition, and the microbial composition changed greatly in different periods. The phylogenetic tree derived from 16s rDNA sequence was found that the closest relatives belong to Clostridium sp., Brevibacillus sp., Bartonella sp.、Bacteroidetes sp.

5d后稻秆总减重量达到39.6%,在培养基占总体积1/5、pH=6、培养第5天时纤维素内切酶活性表现最高,达到14IUml^(-1);培养过程中发酵液中有10余种挥发性产物,且不同时期产物的种类和浓度变化很大,从DGGE图谱发现在培养不同时期菌种组成有很大差异,通过各条带近缘种16s rDNA扩增信息构建系统树可见,各条带近缘种分别归属Clostridium、Brevibacillus、Bartonella、Bacteroidetes4个属。

The circumscription of the Minaceae, phylogenetic relationships of genera and its reative were evaluated using morphology and molecular data of rbcL and trnL-F gene of the chloroplast genome.A total of 4 genera and 19 taxa were examined in this study.And some data of Bryaceae were downloaded from Genebank for this analysis. Phylogenetic trees obtained from neighbour jioning and maximum parsimony methods.

本研究用直接测序法测定了提灯藓科4属19种植物的rbcL和trnL-F基因序列,并从Genebank中下载了该科和真藓科的部分基因序列,采用邻接法和最大简约法分别构建了rbcL和trnL-F两个基因片段的系统树,并结合形态方面的分支分析,探讨了提灯藓科的范畴、科内各属的亲缘关系及与其临近科间的关系。

In order to direct the construction of plant germplasms by elucidating the relatives among plants at the level of gene, CYP86MF gene analogues from 11 species of 6 genera in Cuciferae were respectively obtained by PCR strategy using gene specific primers designed from conserved regions of CYP86MF gene reported. Sequence comparisonindicated that the similarities among the genes at nucleotide level were over 80%, and the similarities at amino acid level remained above 70%. The differences between the genes at nucleotide and amino acid level between species were 1.0%~ 5.7% and 2.6%~ 7.3% respectively, while those between genera 5.6%~ 22.5% and 7.3%~ 31.2%, respectively. Phylogenetic analysis showed that Brassica was closely related to Raphanus, followed by Rorippa Scop, Arabidopsis Heynh, Capsella Medic orderly, most distantly related to Orychophrogmus.

为了从分子水平阐明十字花科植物间的亲缘进化关系,给植物种质资源的创建提供理论依据,试验根据课题组已报道的CYP86MF基因编码的氨基酸保守区域设计特异引物,运用PCR技术分别从十字花科6个属11个物种中分离克隆到了CYP86MF基因的同源序列,经比较分析,结果表明:这些同源序列的相似性达80%以上,所推导的氨基酸序列相似性达70%以上,且两者种间差异分别为1.0%~ 5.7%和2.6%~ 7.3%,属间差异分别是5.6%~ 22.5%和7.3%~ 31.2%;由氨基酸序列构建的分子系统树可知,在亲缘进化关系上芸薹属与萝卜属较近,其他依次为蔊菜属、拟南芥属、荠菜属,而与诸葛菜属最远。

Canis lupus chanco ; mtDNA ; Molecular phylogenetic tree ; Subspecies

中国狼;线粒体DNA ;分子系统树;亚种

Based on Kimura-2-Parameter(K2P) distance,bootstrapped NJ tree(1000 replicates) was generated from these 9 cytb gene sequences of Clupeidae fishes.

结果表明:(1)用Ne ighbour-Join ing法构建的分子系统树显示盖纹沙丁鱼属的3种鱼类形成一个单系类群,鲱属2种鱼类形成一个单系类群,它们的bootstrap支持率均为100%。

The molecular phylogenetic trees showed that the Coliadinae is not monophyletic, but the genus Catopsilia and Colias of this subfamily are grouped together with strong support in both of the NJ and bayesian trees.

分子系统树显示:黄粉蝶亚科不是单系群,但其中迁粉蝶属和豆粉蝶属在不同的分析方法中均聚合在一起。

The molecular phylogeny of the concolor gibbons agree with the opinions on subspecies by morphologists, and 5 evolutionarily significant units might exist in this group, which should be paid attention and need further studies for conservation of gibbons in China.

黑冠长臂猿的分子系统树揭示该类群可能有5个进化显著单元,在遗传管理中应作为不同的保护单元。

The mitochondrial COⅠ gene sequences of 28 species of Cypraeidae including those of 11 species newly determined were used to reconstruct their phylogenetic trees using the neighbor-joining,maximum parsimony and Bayesian methods,aiming to clarify their phylogenetic relationships.

新测了分布于我国海南沿海的11种宝贝科软体动物的线粒体COⅠ基因序列,结合GenBank中17种该科动物的同源性序列片段,基于不同的数学模型,以邻接法、最大简约法和贝叶斯法构建了宝贝科分子系统树

After the multiple sequence alignments, the sequence variation analyses were conducted and the molecular phylogenetic trees of Cypraeidae were reconstructed with NJ, MP and bayesian methods, using Prosimnia semperi of Cypraeacea as an outgroup.

其次,用自测的宝贝科11种软体动物的mtDNA COI基因序列,结合得自GenBank上的18种宝贝科软体动物的同源序列,用NJ法、ML法以及贝叶斯法构建了分子系统树

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。