系出

Other three alien translocation lines referring to chromosome Lr.14 of L.racemosus were also induced via irradiated pollens.After irradiated by gamma raysof 1KR does,the pollens of T.aestivum-L.racemosus Lr.14 disomic addition lineswere used to pollinate wheat varieties"Yangmai No.5"and"Mianyang No.11".M1plants have pairing between wheat chromosomes and Lr.14 ofL.racemosus at PMCMI stage were self-pollinated,and the M2 seeds used to be Giemsa C-banded andfluorescence in situ hybridized.As the result,three alien translocation lines wereobtained and T12 was observed to have distal translocation chromosomes betweenLr.14S and a wheat chromosome segment(TW-Lr.14S·Lr.14L).T13 wasidentified to contain mostly-alien-translocation chromosome(TLr.14L·Lr.14S-W).T14 was proved to contain a translocation between Lr.14L and 6BS,with approximatebreakpoint at 6BS2.2 region, FL0.22 in the short arm of chromosome T6BL.6BS-Lr.14L.

用普通小麦-大赖草Lr.14二体异附加系经辐射处理过的花粉给扬麦5号和绵阳11号授粉，M1代植株进行PMC MI染色体配对分析和Giemsa C-分带，选出PMCMI小麦和大赖草染色体发生配对的植株进行自交，M2代经RTC M期染色体C-分带和原位杂交，鉴定出3个小麦-大赖草易位系，其中T12为由Lr.14大部分和一个小麦染色体片段组成的易位染色体(TLr.14L·Lr.14S-W)；T13含由Lr.14染色体绝大部分与小麦染色体的一个小片段组成的易位染色体(TLr.14L·Lr.14S-W)；T14易位系的染色体易位发生在小麦6BS2.2区和大赖草Lr.14L之间，易位断点在T6BL·6BS-Lr.14L易位染色体的FL0.22附近。

The signals look like noncontinuous beads on the DNA fiber,which consist of multi-copy and arrange tandemly.Fiber-FISH results with 45S rDNA probe showed that an average signal length is about 3～11μm in many tandem copy sequence(measure number,n=8),so we estimated the size of each copy to be approximately 11～30kb in cotton genome.There are dual signals on each end of the mitosis metaphase chromosomes and pachytene chromosomes hybridized with telomere DNA probe.The signals also look like non-continuous beads on the DNA fiber,the length is about 1～9μm,so we estimated the size is about 4～27kb.The signals almost covered the whole mitosis metaphase chromosomes and pachytene chromosomes of G.arboretum Shixiya 1 hybridized with gDNA probe,the euchromatin zone and heterochromatin zone were identified clearly on pachytene chromosomes,and the signals also look like non-continuous beads.Two BAC clones 150-D-24 and 182-F-9 in DNA BAC library of Pima90 were selected as probe to hybridize with mitosis metaphase chromosomes,pachytene chromosomes and DNA fiber of G.raimondii.

棉花FISH技术系统的应用研究。45S rDNA在亚洲棉石系亚1号中期和粗线期染色体上有两对大的信号，在DNA纤维上信号为非连续的念珠状，多个拷贝串联排列，每个拷贝长度大约在3～11μm，推测每个拷贝的实际长度为11～30kb；端粒序列在亚洲棉石系亚1号中期和粗线期染色体端部都有双点信号，在DNA纤维上信号也为非连续的念珠状，不同染色体上信号长度大约在1～9μm不等，推测实际长度为4～27kb；gDNA信号几乎布满整个亚洲棉石系亚1号中期和粗线期染色体，在粗线期染色体上能明显区分出常染色质区和异染色质区，DNA纤维上的信号均为非连续的念珠状。

Objective: Presymptomatic gene diagnosis of autosomal dominant polycystic kidney disease families by PCR Methods: Genomic DNA were extracted Highly polymorphic microsatellite markers(SM7, AG2 5, KG8, CW2) linked to the PKD1were amplified using PCR Ten ADPKD families (104 members,including 28 affected) were analysed Results: Nine children were diagnosed with presymptomatic gene diagnosis ,although all children presented a negative ultrasonic diagnosis Conclusion: The presymptomatic gene diagnosis ...

目的：应用PCR方法对常染色体显性遗传性多囊肾病家系进行症前基因诊断。方法：用PCR扩增与PKD1位点连锁的的高度多态性的微小卫星体DNA（SM7，AC2.5，KG8，CW2）为遗传标记，对10个ADPKD家系的104个成员（包括28个患者）找出染色体上与疾病连锁的单体型，进行连锁分析。结果：对9个无临床症状，且B超检查呈阴性结果的儿童做出了症前基因诊断。结论：能够应用PCR方法对ADP－KD家系成员快速、准确地做出基因诊断

Based on the pyrocondensation model and principles of elasticity, the layer structure design of thin films was proposed in view of the control of residual thermal stress and the corresponding evaluation function was applied to the layer structure design of films.

基于热收缩效应模型和弹性力学原理，从薄膜膜系着手提出了控制薄膜残余热应力的膜系设计原则，将该原则的评价函数用于薄膜膜系设计中，采用优化方法可以设计出残余热应力可接受的薄膜。

The results indicated that an improved 1BL/1RS translocation has Glu-B3 and Yr9 without secalin,and has higher SDS-sedmentation value than common 1BL/1RS translocation.

结果表明，在87份材料中鉴定出了一种新的改良1BL/1RS易位系，该类易位系的特征是：含有来自1RS的抗条锈病基因Yr9、不含黑麦碱、具有Glu-B3基因、比一般1BL/1RS易位系具有明显偏高的SDS-沉降值。

In different treatments there were significant differences in male sterility among INACs, of which I TA showed the highest rate of sterility and the best sterility stability under different conditions; meanwhile, altering nucleo cytoplasmic relationship by substitutional backcrosses could modify and improve male sterility of CMS lines.

结果表明，同核异质不育系不育度在年份间、播期间、实生稻与再生稻间差异极显著，不同细胞质不育系间差异显著，其中I特A在各种处理条件下均表现出最高的不育度和最好的不育稳定性。通过回交核置换技术培育新核质关系的同核异质不育系，可达到修饰改良育性的目的。

Lodicules of the CMS lines with lighter CGG had more, bigger and unequal tracheae in SVB, and had bigger volume, heavier weight and lower water potential compared with the CMS lines with CGG. Similar to CMS lines, the B-lines had similar changes.

与种子裂颖重的不育系相比，种子裂颖轻的不育系浆片小维管束导管面积大、数目多且分布均匀、体积小、重量轻、水势低；且相应保持系也表现出大致相同的规律。

Secondly, in the dissertation, the theories and methods of calculation on bridge deck system were discussed, and bridge deck system simplified as a beam was analyzed with the theory of elastically supported continuous beam and the method of deformation compatibility; through the theory of orthotropic plate being applied to analyzing the bridge deck system which was assumed an elastically supported continuous plate, certain theoretical derivations were made and some calculation formulas were given.

其次，本文研究论述了桥道系的计算理论和方法，将桥道系简化为梁，利用弹性支承连续梁理论和变形协调法进行分析；把桥道系视为弹性支承连续板，利用正交异性板理论进行分析，作了一定的理论推导，并给出了相关计算公式。

It is revealed that the asymmetric vortices over slender body are formed the breakaways of higher position vortex and generation of new vortex, which are alternately developed on the both sides along the body axis. The correlation between asymmetric vortices structure and distribution of sectional sideforce coefficient has been obtained. A physical model of asymmetric multi-vortices flow structure is developed according to zonal flow analysis.

在此基础上揭示了非对称涡系是通过细长体两侧背涡沿轴向交替脱落、新生而形成的，给出了非对称涡系沿轴向演化、发展的流动结构和截面侧向力分布特性之间的相关关系，在分区分析的基础上提出了非对称涡系结构的物理模型。

The results showed that the respiration rate of J1Jm (Japan♀×Japan♂) and C1Jm (China♀×Japan♂) increased with increase in water temperature, but significantly decreased at over 18℃. The respiration rate in "Chinese red" and its half-sib families (C1Rh, J7Rh, RjRh) sharply decreased at 21℃, indicating that the "Chinese red" and its half-sib families adopted higher temperature. The changes in NH4-N excretion rate showed a similar tendency to the respiration during the experiment.

结果表明：J1Jm（皱纹盘鲍日本群体♀×日本群体♂）和C1Jm（皱纹盘鲍中国群体♀×日本群体♂）的耗氧率随水温的升高而上升，但超过18℃在21℃下耗氧率明显下降，RwRh与其半同胞家系(C1Rh、J7Rh、RjRh)的耗氧率拐点出现在21℃，说明&中国红&及其半同胞家系更适宜较高温度；各家系排氨率随温度升高的变化趋势与耗氧率基本相同。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷