糠秕

Methods Patients with acne were checked during clinical conventional examination,for the presence of tollicle flocculence and chaff spore bacteria,by examining with microscope the sebum or follicular keratotic plug from the skin lesion after10%KOH was added.

在门诊痤疮患者中常规检查毛囊虫和糠秕孢子菌，于皮损上挤出少量皮脂或毛囊角栓，置于玻片上，加10%KOH，盖上盖玻片，在显微镜下检查。

Object To found a different cDNA library with Malasesiza furfur in hypha phase, and procure its gene fragment on differential expression.

目的：建立糠秕马拉色菌菌丝相细胞的差异表达cDNA文库，获得其差异表达的基因片段。

Found a different cDNA library with Malasesiza furfur in hypha phase by Subtractive Hybridization; And sequencing some fragment of differential expression gene. Provid foundation for the further study on complete differential gene expression.

采用消减杂交技术建立了糠秕马拉色菌的差异cDNA文库；并对部分克隆片段进行测序，为今后获得完整的差异表达基因打下基础。

Many reports suggested that Malassezia could cause Pityriasis Versicolor,Seborrheic Dermatitis,Malassezia furfur Folliculitis and Psoriasis et al.These can be cured by anti-fungus medicine.

大量研究表明，马拉色菌可引起花斑癣、脂溢性皮炎、糠秕孢子菌毛囊炎和银屑病等，对于这些疾病的治疗可加用抗真菌药物。

Furfur was inoculated to the mycelial culture liquid medium and the rapeseed oil culture liquid medium, and then incubated in vibrator to induce the production of mycelial phase and yeast phase of M.

用全脂牛奶平板法、Folin-酚法、QuantiCleave蛋白酶试剂盒测定菌丝相及酵母相糠秕马拉色菌胞外蛋白酶的活力，并检测温度、pH值对蛋白酶活性的影响。

Results: The autofluorescence of M. furfur and M. japonica standard trainsrespectively in 4th and 6th day are stronger than that in other days, andautofluorescence of colonies become weaker in an hour. Theautofluorescence of Malassezia colonies put in 3% glutaraldehyde, pH4.0HCL, 1280μg/mL and 64μg/mL fluconazol solution, 0.3% methylene blueseparately get stronger, and that in 10% KOH get stronger at beginning thenbecome weaker 2 days later. C. albicans, S. schenchii, T. rubrum, T.tonsurans, A. terreus, A. fumigatus cultured on SDA all haveautofluorescence, and which are weaker than that of Malassezia.

结果：糠秕马拉色菌和日本马拉色菌2株标准株的自发荧光强度分别在培养第4和8天时最强，菌落的自发荧光在1小时内逐渐减弱；3％戊二醛溶液、pH4.0盐酸溶液、1280μg／mL和64μg／mL氟康唑溶液、3％美蓝溶液处理后马拉色菌自发荧光都增强，10％氢氧化钾溶液中的马拉色菌在开始时荧光增强，2天后减弱；白念珠菌、申克孢子丝菌、红色毛癣菌、断发毛癣菌、土曲霉、烟曲霉等在培养条件下有自发荧光，但都较马拉色菌弱。

First, to isolate the total RNA both in hypha and yeast phase of Malasesiza furfur, Then reverse them to cDNA and found a different cDNA library with Malasesiza furfur in hypha phase by Subtractive Hybridization; At last, clone and sequencing the gene fragment on differential expression of Malasesiza furfur in hypha phase.

首先提取糠秕马拉色菌两相细胞的总RNA；然后逆转录生成两相细胞cDNA，运用消减杂交技术获得菌丝相细胞差异表达的cDNA，并建立差异文库：最后对差异cDNA片段克隆测序。

To explore the possibility of early and rapid diagnosis of mycosisby detecting the fungal autofluorescence. Methods: To culture M. furfur and M. japonica standard strains on Dixonmedium at 32℃, and to study the autofluorescence of colonies in 2nd, 4th, 6th, 8th, 10th day under Laser Scanning Confocal Microscopy (LeicaTCS-SP2 LSCM). To study the variation of autofluorescence whenMalassezia colonies were put in 3% glutaraldehyde, pH4.0 HCL, 10% KOH, fluconazol solution, 0.3% methylene blue separately. To detect theautofluorescence of C. albicans, S. schenchii, T. rubrum, T. tonsurans, A.terreus, A. fumigatus cultured on Sabouraud dextrose agar.

选用糠秕马拉色菌和日本马拉色菌2株标准菌，接种在Dixon培养基32℃培养，分别在培养的第2、4、6、8、10天挑取菌落放在TCS-SP2型激光扫描共聚焦显微镜(Leica TCS-SP2 LSCM)下观察、测定其自发荧光；分别用3％戊二醛溶液、pH4.0盐酸溶液、10％氢氧化钾溶液、1280μg／mL和64μg／mL氟康唑溶液、0.3％美蓝溶液处理后观察马拉色菌自发荧光的变化；选择白念珠菌、申克孢子丝菌、红色毛癣菌、断发毛癣菌、土曲霉、烟曲霉等用沙堡葡萄糖琼脂培养后观察其自发荧光的特点。

"This oil-loving yeast causes pityriasis versicolor, scaling and discoloration of the skin and scalp."

嗜脂性的糠秕孢马拉色菌会引起花斑癣，其症状是皮肤表面覆盖细薄糠状鳞屑以及色素脱失的斑点。

Objective:①To study the extracellular proteinase activity of M.

目的：①检测糠秕马拉色菌胞外蛋白酶活性；②研究菌丝相及酵母相蛋白酶活性的差异；③蛋白酶活性的影响因素。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。